Macrobrachium olfersii
Contents
Description
- The freshwater prawn (Macrobrachium olfersii) has wide distribution in the Americas and is emerging as a potential model for development, developmental toxicity and environmental toxicology studies.Macrobrachium olfersii exhibits physiological characteristics typical of a recent freshwater invader, but is still dependent on brackish water for larval development and metamorphosis
- The embryonic development of Macrobrachium olfersii has been well-characterized both morphologically and chronologically. However, the molecular mechanisms of this species, including its development, particularly in the embryonic stages, remained unknown. [1] [2]
Different Developmental Stages
Reference Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primer | Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| RpL8[1] | ribosomal protein L8 (RpL8) |
|
KY027065 |
|
125 | 60 | GoTaq |
| RpS6[1] | Ribosomal protein S6 (RpS6) |
|
KY027064 |
|
78 | 60 | GoTaq |
Moleculer Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
- Ref-Finder method && Related Reference
- ΔCt approach method && Related Reference
Contact
- Name: Evelise M. Nazari
- Email: evelise.nazari@ufsc.br
- Institution: Universidade Federal de Santa Catarina, Departamento de Biologia Celular, Embriologia e Genética, 88040-900 Florianópolis, Santa Catarina, Brazil
Citation Statistics
Cited by 0 (Based on Google Scholar [2017-06-01])
References
- ↑ 1.0 1.1 1.2 Jaramillo ML, Ammar D, Quispe RL, Guzman F, Margis R, Nazari EM, Müller YM. Identification and evaluation of reference genes for expression studies by RT-qPCR during embryonic development of the emerging model organism, Macrobrachium olfersii. Gene. 2017 Jan 20;598:97-106. doi: 10.1016/j.gene.2016.11.001. Epub 2016 Nov 5. PubMed PMID: 27825774.
- ↑ Mendonça NN, Masui DC, McNamara JC, Leone FA, Furriel RP. Long-term exposure of the freshwater shrimp Macrobrachium olfersii to elevated salinity: effects on gill (Na+,K+)-ATPase alpha-subunit expression and K+-phosphatase activity. Comp Biochem Physiol A Mol Integr Physiol. 2007 Apr;146(4):534-43. Epub 2006 Mar 10. PubMed PMID: 16529963.
