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Welcome to ICG A knowledgebase of Internal Control Genes for RT-qPCR normalization

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ICG is a wiki-based knowledgebase of internal control genes (or reference genes) for RT-qPCR normalization in a variety of species. Based on community curation, ICG harnesses collective intelligence to integrate a comprehensive collection of internal control genes curated from a large volume of literatures and provides appropriate internal control genes corresponding to specific experimental conditions for both model and non-model organisms. Nothing great is ever accomplished in isolation. – Yo-Yo Ma

Recent Updates Domestic dog Canis lupus familiaris Oriental fruit fly Bactrocera dorsalis Common marmoset Callithrix jacchus Striped dolphin Stenella coeruleoalba Wormwood Artemisia annua Neurospora crassa Neurospora crassa Ginseng Panax ginseng Lingzhi Ganoderma lucidum Alfredii Sedum alfredii Peanut Arachis hypogaea   >> More...

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News 7 miRNA-related projects describing internal control genes for RT-qPCR normalization are included in ICG ICG-version 1.0 is online A total of 209 species are integrated in ICG, including 73 Animals, 115 Plants, 12 Fungi and 9 Bacteria More than 70 kinds of experimental conditions are included, such as Salinity, Heat and Insecticide Treatment 757 internal control genes with experimental validation are curated, such as EF1α, Ubiqutin and ACT More than 200 literatures on Selection of Internal Control Genes are curated The second round of investigation research on ICG project is completed The first round of investigation research on ICG project is completed

To be curated Species Literature Prunus persica Marini N, Bevilacqua C B, Büttow M V, et al. Identification of reference genes for RT-qPCR analysis in peach genotypes with contrasting chilling requirements. Genetics and molecular research: GMR, 2017, 16(2). Burkholderia pyrrocinia Wu B Y, Ye J R, Huang L, et al. Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007. Journal of microbiological methods, 2017, 132: 95-98. Shiraia bambusicola Zhang C, Li T, Hou C L, et al. Selection of reference genes from Shiraia bambusicola for RT-qPCR analysis under different culturing conditions. AMB Express, 2017, 7(1): 14. Poa pratensis Niu K, Shi Y, Ma H. Selection of candidate reference genes for gene expression analysis in Kentucky Bluegrass (Poa pratensis L.) under abiotic stress. Frontiers in plant science, 2017, 8.

Joining ICG ICG allows any user to view and search but only registered users can add and edit content. To become a registered user, please email the ICG Team at haolili@big.ac.cn to tell us your preferred login name, real name, research interests, etc., and we will set up an account for you. Please also spread this news to any one who might be of interest.

How to Cite Biologists are always welcome to use the ICG data or knowedge in their own work. The ICG project has been published in Nucleic Acids Research. To cite us, please refer to the following publication: ICG: a wiki-driven knowledgebase of internal control genes for RT-qPCR normalization. Nucleic Acids Res. 2018. 46(D1), D121-D126. PubMed PMID:29036693.

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