Difference between revisions of "Mus musculus"
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ICG Expert1 (talk | contribs) (Created page with "=='''Mouse Dextran Sodium Sulfate Experimental Colitis'''== ===Reference Genes=== {|class="wikitable sortable" style="font-size:10pt; width:100%" |- ! Gene symbol ! Gene na...") |
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− | =='''Mouse | + | =='''Description'''== |
+ | [[File:Mus musculus.png|right|200px|link=Mus musculus]] | ||
+ | * '''''Mus musculus''''' is a small mammal of the order Rodentia, characteristically having a pointed snout, small rounded ears, and a long naked or almost hairless tail. It is one of the most numerous species of the genus Mus. Although a wild animal, the house mouse mainly lives in association with humans [https://en.wikipedia.org/wiki/House_mouse (Modified from Wikipedia)]. | ||
+ | * <font color=blue>'''Common Name:'''</font> '''Mouse''' | ||
+ | * [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10090 <font color=blue>'''NCBI Taxonomy'''</font>] | ||
− | === | + | =='''''Dextran Sodium Sulfate Experimental Colitis'''''== |
+ | |||
+ | ===Internal Control Genes=== | ||
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 16: | Line 22: | ||
|align="center"| TATA-box-binding protein | |align="center"| TATA-box-binding protein | ||
| | | | ||
− | *Mouse | + | *Mouse dextran sodium sulfate experimental colitis |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/172073170 '''NM_013684 '''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/172073170 '''NM_013684 '''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 28: | Line 34: | ||
|align="center"| Eukaryotic Translation Elongation Factor 2 | |align="center"| Eukaryotic Translation Elongation Factor 2 | ||
| | | | ||
− | *Mouse | + | *Mouse dextran sodium sulfate experimental colitis |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_007907.2 '''NM_007907.2'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_007907.2 '''NM_007907.2'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 38: | Line 44: | ||
|} | |} | ||
− | === | + | ===Molecular Types=== |
* mRNA | * mRNA | ||
Line 48: | Line 54: | ||
*'''Name''': Jean-Eric Ghia | *'''Name''': Jean-Eric Ghia | ||
*'''Email''': Jean-Eric.Ghia@umanitoba.ca | *'''Email''': Jean-Eric.Ghia@umanitoba.ca | ||
− | *''' | + | *'''Institution''': Immunology, University of Manitoba, Winnipeg, MB, Canada |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=15094739600394943508&as_sdt=2005&sciodt=0,5&hl=en '''3''' ](Based on Google Scholar [2017-09-01]) | ||
− | ==''' | + | =='''''Mammary Glands'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt;width:100%;" | {|class="wikitable sortable" style="font-size:10pt;width:100%;" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 64: | Line 72: | ||
|- | |- | ||
|align="center"| HPRT<ref name="ref2"/> | |align="center"| HPRT<ref name="ref2"/> | ||
− | |align="center"| | + | |align="center"| Hypoxanthine guanine phosphoribosyl transferase |
| | | | ||
− | * | + | * Mouse mammary glands |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013556 '''NM_013556'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013556 '''NM_013556'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 76: | Line 84: | ||
|- | |- | ||
|align="center"| RPL13A<ref name="ref2"/> | |align="center"| RPL13A<ref name="ref2"/> | ||
− | |align="center"| | + | |align="center"| Ribosomal protein L13A |
| | | | ||
− | * | + | * Mouse mammary glands |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_009438 '''NM_009438'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_009438 '''NM_009438'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 88: | Line 96: | ||
|- | |- | ||
|align="center"| GAPDH<ref name="ref2"/> | |align="center"| GAPDH<ref name="ref2"/> | ||
− | |align="center"| | + | |align="center"| Glyceraldehyde-3-phosphate dehydrogenase |
| | | | ||
− | * | + | * Mouse mammary glands |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008084 '''NM_008084'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008084 '''NM_008084'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 100: | Line 108: | ||
|} | |} | ||
− | === | + | ===Molecular Types=== |
* mRNA | * mRNA | ||
Line 109: | Line 117: | ||
*'''Name''': Y.L. Wang | *'''Name''': Y.L. Wang | ||
*'''Email''': ylwang2001@yahoo.cn | *'''Email''': ylwang2001@yahoo.cn | ||
− | *''' | + | *'''Institution''': College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=3139211935715979100&as_sdt=2005&sciodt=0,5&hl=en '''11'''] (Based on Google Scholar [2017-09-01]) | ||
− | ==''' | + | =='''''Uterus in the Peri-Implantation Period'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 127: | Line 137: | ||
|align="center"| Ribosomal protein, large, P0 | |align="center"| Ribosomal protein, large, P0 | ||
| | | | ||
− | * | + | * Early pregnancy, pseudopregnancy, delayed implantation |
− | * | + | * Activation, artificial decidualization |
− | * | + | * Hormonal treatment model |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_007475 '''NM_007475'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_007475 '''NM_007475'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 141: | Line 151: | ||
|align="center"| Glyceraldehydes-3-phosphate dehydrogenase | |align="center"| Glyceraldehydes-3-phosphate dehydrogenase | ||
| | | | ||
− | * | + | * Early pregnancy, pseudopregnancy, delayed implantation |
− | * | + | * Activation, artificial decidualization |
− | * | + | * Hormonal treatment model |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008084 '''NM_008084'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008084 '''NM_008084'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 153: | Line 163: | ||
|} | |} | ||
− | === | + | ===Molecular Types=== |
* mRNA | * mRNA | ||
Line 164: | Line 174: | ||
*'''Name''': AiHua Wang | *'''Name''': AiHua Wang | ||
*'''Email''': pflin2001n@163.com | *'''Email''': pflin2001n@163.com | ||
− | *''' | + | *'''Institution''': College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=1151953663364672682&as_sdt=2005&sciodt=0,5&hl=en '''28'''] (Based on Google Scholar [2017-09-01]) | ||
− | ==''' | + | =='''''Different Tissues & Ageing'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! style="width=20% font-size:9pt "| Gene | + | ! style="width=20% font-size:9pt "| Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 180: | Line 192: | ||
|- | |- | ||
|align="center"| Rpl13a<ref name="ref4"/> | |align="center"| Rpl13a<ref name="ref4"/> | ||
− | |align="center"| | + | |align="center"| Ribosomal protein L13a |
| | | | ||
− | * | + | * Cerebral cortex during ageing |
− | * | + | *Cortex during ageing |
− | * | + | *Hippocampus during ageing |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_009438.5 '''NM_009438.5'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_009438.5 '''NM_009438.5'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
− | * F: | + | * F:TACGCTGTGAAGGCATCAAC |
− | * R: | + | * R:GGGAGGGGTTGGTATTCATC |
|align="center"| 96 | |align="center"| 96 | ||
|align="center"| 60 | |align="center"| 60 | ||
Line 194: | Line 206: | ||
|- | |- | ||
|align="center"| GAPDH<ref name="ref4"/> | |align="center"| GAPDH<ref name="ref4"/> | ||
− | |align="center"| | + | |align="center"| Glyceraldehyde-3-phosphate dehydrogenase |
| | | | ||
− | * | + | *Cerebral cortex during ageing |
− | * | + | *Cortex during ageing |
− | * | + | *Cerebellum during ageing |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008084.3 '''NM_008084.3'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008084.3 '''NM_008084.3'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
− | * F: | + | * F:TGCGACTTCAACAGCAACTC |
− | * R: | + | * R:ATGTAGGCAATGAGGTCCAC |
|align="center"| 143 | |align="center"| 143 | ||
|align="center"| 60 | |align="center"| 60 | ||
Line 208: | Line 220: | ||
|- | |- | ||
|align="center"| Ppib<ref name="ref4"/> | |align="center"| Ppib<ref name="ref4"/> | ||
− | |align="center"| | + | |align="center"| Peptidylprolyl isomerase (Cyclophilin) B |
| | | | ||
− | * | + | *Striatum during ageing |
− | * | + | *Cerebellum during ageing |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_011149.2 '''NM_011149.2'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_011149.2 '''NM_011149.2'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
− | * F: | + | * F:CAGCAAGTTCCATCGTGTCA |
− | * R: | + | * R:GATGCTCTTTCCTCCTGTGC |
|align="center"| 85 | |align="center"| 85 | ||
|align="center"| 60 | |align="center"| 60 | ||
Line 221: | Line 233: | ||
|- | |- | ||
|align="center"| Hprt<ref name="ref4"/> | |align="center"| Hprt<ref name="ref4"/> | ||
− | |align="center"| | + | |align="center"| Hypoxanthine guanine phosphoribosyl transferase |
| | | | ||
− | * | + | *Hippocampus during ageing |
− | * | + | *Cerebellum during ageing |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013556.2 '''NM_013556.2'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013556.2 '''NM_013556.2'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
− | * F: | + | * F:CTTTGCTGACCTGCTGGATT |
− | * R: | + | * R:TATGTCCCCCGTTGACTGAT |
|align="center"| 121 | |align="center"| 121 | ||
|align="center"| 60 | |align="center"| 60 | ||
Line 234: | Line 246: | ||
|} | |} | ||
− | === | + | ===Molecular Types=== |
* mRNA | * mRNA | ||
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*'''Name''': B.D.Roussel | *'''Name''': B.D.Roussel | ||
*'''Email''': broussel@cyceron.fr | *'''Email''': broussel@cyceron.fr | ||
− | *''' | + | *'''Institution''': INSERM, INSERM U919, Serine Proteases and Pathophysiology of the Neurovascular Unit, GIP CYCERON, University Caen Basse Normandie, boulevard Becquerel, 14074 Caen, France |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=1382777012639468795&as_sdt=2005&sciodt=0,5&hl=en '''6'''] (Based on Google Scholar [2017-09-01]) | ||
− | ==''' | + | =='''''Lung Development'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 260: | Line 274: | ||
|- | |- | ||
|align="center"| Tuba1a<ref name="ref5"/> | |align="center"| Tuba1a<ref name="ref5"/> | ||
− | |align="center"| | + | |align="center"| Alpha 1a tubulin |
| | | | ||
− | * | + | *Different stages of lung development |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_011653 '''NM_011653 '''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_011653 '''NM_011653 '''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 272: | Line 286: | ||
|} | |} | ||
− | === | + | ===Molecular Types=== |
* mRNA | * mRNA | ||
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*'''Name''': Guillermo Barreto | *'''Name''': Guillermo Barreto | ||
*'''Email''': guillermo.barreto@mpi-bn.mpg.de | *'''Email''': guillermo.barreto@mpi-bn.mpg.de | ||
− | *''' | + | *'''Institution''': LOEWE Research Group Lung Cancer Epigenetic, Max-Planck-Institute for Heart and Lung Research, Parkstraße 1, 61231 Bad Nauheim, Germany |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=17284368679653769355&as_sdt=2005&sciodt=0,5&hl=en '''7'''] (Based on Google Scholar [2017-09-01]) | ||
− | =='''Epithelial | + | =='''''Epithelial & Nonepithelial Cells'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 299: | Line 315: | ||
|align="center"| Beta-glucuronidase | |align="center"| Beta-glucuronidase | ||
| | | | ||
− | * mouse | + | * Eepithelial cells isolated from the mouse intestine |
+ | * Eepithelial cells isolated the IEC6 cell line | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_010368 '''NM_010368'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_010368 '''NM_010368'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 309: | Line 326: | ||
|- | |- | ||
|align="center"| TBP<ref name="ref6"/> | |align="center"| TBP<ref name="ref6"/> | ||
− | |align="center"| | + | |align="center"| TATA-box binding protein |
| | | | ||
− | * mouse | + | * Eepithelial cells isolated from the mouse intestine |
+ | * Eepithelial cells isolated the IEC6 cell line | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008907 '''NM_008907'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008907 '''NM_008907'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 320: | Line 338: | ||
|align="center"| SYBR | |align="center"| SYBR | ||
|} | |} | ||
− | === | + | |
+ | ===Molecular Types=== | ||
* mRNA | * mRNA | ||
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*'''Name''': Yongping Su | *'''Name''': Yongping Su | ||
*'''Email''': suyp2003@yahoo.com.cn | *'''Email''': suyp2003@yahoo.com.cn | ||
− | *''' | + | *'''Institution''': Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Road, Shapingba District, Chongqing 400038, China |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=15259891202787233691&as_sdt=2005&sciodt=0,5&hl=en '''55'''] (Based on Google Scholar [2017-09-01]) | ||
− | =='''Preimplantation Embryos'''== | + | =='''''Preimplantation Embryos'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! style="width=15% font-size:9pt "|Gene | + | ! style="width=15% font-size:9pt "|Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 349: | Line 370: | ||
| | | | ||
* B6D2F-1 and C57BL/6 strains | * B6D2F-1 and C57BL/6 strains | ||
− | * | + | * Blastocyst embryonic stages cultured in KSOM medium |
*KOSOM and CZB cultures of B6D2F-1 and C57BL/6 strain-derived embryos | *KOSOM and CZB cultures of B6D2F-1 and C57BL/6 strain-derived embryos | ||
− | * | + | * Zygote stage embryos |
*2-cell, 4-cell, 8-cell stage embryos cultured in M16 medium | *2-cell, 4-cell, 8-cell stage embryos cultured in M16 medium | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_016750 '''NM_016750 '''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_016750 '''NM_016750 '''] | ||
Line 362: | Line 383: | ||
|- | |- | ||
|align="center"| sptbn<ref name="ref7"/> | |align="center"| sptbn<ref name="ref7"/> | ||
− | |align="center"| | + | |align="center"| Pectrin beta, non-erythrocytic 1 |
| | | | ||
* ICR strain-derived embryos | * ICR strain-derived embryos | ||
Line 379: | Line 400: | ||
* C57BL/6 strain-derived embryos | * C57BL/6 strain-derived embryos | ||
* 2-cell, 4-cell stage embryos cultured in CZB medium | * 2-cell, 4-cell stage embryos cultured in CZB medium | ||
− | * | + | * Blastocyst stage embryos cultured in M16 medium |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_030215 '''NM_030215 '''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_030215 '''NM_030215 '''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 389: | Line 410: | ||
|- | |- | ||
|align="center"| ywhaz<ref name="ref7"/> | |align="center"| ywhaz<ref name="ref7"/> | ||
− | |align="center"| | + | |align="center"| 14-3-3 protein zeta/delta (14-3-3ζ) |
| | | | ||
− | * | + | * Zygote & molular embryonic stages cultured in KSOM medium |
− | * | + | *Molular stage embryos cultured in CZB medium |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_001253807 '''NM_001253807'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_001253807 '''NM_001253807'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 402: | Line 423: | ||
|- | |- | ||
|align="center"| tgfb1<ref name="ref7"/> | |align="center"| tgfb1<ref name="ref7"/> | ||
− | |align="center"| | + | |align="center"| Transforming growth factor, beta 1 |
| | | | ||
* 2-cell embryonic stages cultured in KSOM medium | * 2-cell embryonic stages cultured in KSOM medium | ||
Line 417: | Line 438: | ||
| | | | ||
* 4-cell, 8-cell embryonic stages cultured in KSOM medium | * 4-cell, 8-cell embryonic stages cultured in KSOM medium | ||
− | * | + | * Zygote embryos cultured in M16 medium |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/X00686 '''X00686 '''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/X00686 '''X00686 '''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 427: | Line 448: | ||
|- | |- | ||
|align="center"| actb<ref name="ref7"/> | |align="center"| actb<ref name="ref7"/> | ||
− | |align="center"| actin | + | |align="center"| β-actin |
| | | | ||
* 8-cell stage embryos cultured in M16 medium | * 8-cell stage embryos cultured in M16 medium | ||
Line 439: | Line 460: | ||
|} | |} | ||
− | === | + | ===Molecular Types=== |
* mRNA | * mRNA | ||
Line 448: | Line 469: | ||
*'''Name''': Jin-Hoi Kim | *'''Name''': Jin-Hoi Kim | ||
*'''Email''': jhkim541@konkuk.ac.kr | *'''Email''': jhkim541@konkuk.ac.kr | ||
− | *''' | + | *'''Institution''': Department of Animal Biotechnology, KonKuk University, Seoul 143-701, Republic of Korea |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=3344230699377018626&as_sdt=2005&sciodt=0,5&hl=en '''4'''] (Based on Google Scholar [2017-09-01]) | ||
− | ==''' | + | =='''''Embryonic Stem Cells'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 464: | Line 487: | ||
|- | |- | ||
|align="center"| Pgk1<ref name="ref8"/> | |align="center"| Pgk1<ref name="ref8"/> | ||
− | |align="center"| | + | |align="center"| Phosphoglycerate kinase 1 |
| | | | ||
− | * | + | * ES, TS and XEN stem cells |
− | * | + | * In vitro differentiation of embryonic and trophectoderm stem cells |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008828 '''NM_008828'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008828 '''NM_008828'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 479: | Line 502: | ||
|align="center"| Succinate dehydrogenase complex, subunit A | |align="center"| Succinate dehydrogenase complex, subunit A | ||
| | | | ||
− | * | + | * ES, TS and XEN stem cells |
− | * | + | * In vitro differentiation of embryonic and trophectoderm stem cells |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/BC011301 '''BC011301'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/BC011301 '''BC011301'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 492: | Line 515: | ||
|align="center"| TATA box binding protein | |align="center"| TATA box binding protein | ||
| | | | ||
− | * | + | * ES, TS and XEN stem cells |
− | * | + | * In vitro differentiation of embryonic and trophectoderm stem cells |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013684 '''NM_013684'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013684 '''NM_013684'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 505: | Line 528: | ||
|align="center"| Tyrosine 3-monooxygenase /tryptophan 5-monooxygenase activation protein, zeta polypeptide | |align="center"| Tyrosine 3-monooxygenase /tryptophan 5-monooxygenase activation protein, zeta polypeptide | ||
| | | | ||
− | * | + | * In vitro differentiation of embryonic and trophectoderm stem cells |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013684 '''NM_013684'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013684 '''NM_013684'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 515: | Line 538: | ||
|- | |- | ||
|align="center"| Hk2<ref name="ref8"/> | |align="center"| Hk2<ref name="ref8"/> | ||
− | |align="center"| | + | |align="center"| Hexokinase 2 |
| | | | ||
− | * | + | * In vitro differentiation of embryonic and trophectoderm stem cells |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_011740 '''NM_011740'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_011740 '''NM_011740'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 526: | Line 549: | ||
|align="center"| SYBR | |align="center"| SYBR | ||
|} | |} | ||
− | === | + | |
+ | ===Molecular Types=== | ||
* mRNA | * mRNA | ||
Line 537: | Line 561: | ||
*'''Name''': Michael C. Golding | *'''Name''': Michael C. Golding | ||
*'''Email''': mgolding@cvm.tamu.edu | *'''Email''': mgolding@cvm.tamu.edu | ||
− | *''' | + | *'''Institution''': College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, United States of America |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=10119274701109814464&as_sdt=2005&sciodt=0,5&hl=en '''42'''] (Based on Google Scholar [2017-09-01]) | ||
− | ==''' | + | =='''''Obesity Model'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 555: | Line 581: | ||
|align="center"| Beta 2 Microglobulin | |align="center"| Beta 2 Microglobulin | ||
| | | | ||
− | * | + | * Liver of monosodium glutamate (MSG)-injected mice with developed central obesity |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_009735 '''NM_009735'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_009735 '''NM_009735'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 567: | Line 593: | ||
|align="center"| 18S Ribosomal RNA | |align="center"| 18S Ribosomal RNA | ||
| | | | ||
− | * | + | * Liver of monosodium glutamate (MSG)-injected mice with developed central obesity |
− | * | + | * Small intestine of monosodium glutamate (MSG)-injected mice with developed central obesity |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NR_003278 '''NR_003278'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NR_003278 '''NR_003278'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 580: | Line 606: | ||
|align="center"| Hypoxanthine phosphoribosyl transferase | |align="center"| Hypoxanthine phosphoribosyl transferase | ||
| | | | ||
− | * | + | *Liver of monosodium glutamate (MSG)-injected mice with developed central obesity |
− | * | + | * Small intestine of monosodium glutamate (MSG)-injected mice with developed central obesity |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013556 '''NM_013556'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013556 '''NM_013556'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 593: | Line 619: | ||
|align="center"| Ribosomal Protein large P0 | |align="center"| Ribosomal Protein large P0 | ||
| | | | ||
− | * | + | * Small intestine of monosodium glutamate (MSG)-injected mice with developed central obesity |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/254939638/ '''NM_007475'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/254939638/ '''NM_007475'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 603: | Line 629: | ||
|} | |} | ||
− | === | + | ===Molecular Types=== |
* mRNA | * mRNA | ||
Line 614: | Line 640: | ||
*'''Name''': Petra Matoušková | *'''Name''': Petra Matoušková | ||
*'''Email''': matousp7@faf.cuni.cz | *'''Email''': matousp7@faf.cuni.cz | ||
− | *''' | + | *'''Institution''': Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=2461273527433039566&as_sdt=2005&sciodt=0,5&hl=en '''26'''] (Based on Google Scholar [2017-09-01]) | ||
− | =='''Testis Development'''== | + | =='''''Testis Development'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 630: | Line 658: | ||
|- | |- | ||
|align="center"| Ppia<ref name="ref10"/> | |align="center"| Ppia<ref name="ref10"/> | ||
− | |align="center"| | + | |align="center"| Peptidylprolyl isomerase (Cyclophilin) A |
| | | | ||
− | * | + | * Testis development |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008907 '''NM_008907'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008907 '''NM_008907'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 642: | Line 670: | ||
|- | |- | ||
|align="center"| Gapdh<ref name="ref10"/> | |align="center"| Gapdh<ref name="ref10"/> | ||
− | |align="center"| | + | |align="center"| Glyceraldehyde-3-phosphate dehydrogenase |
| | | | ||
− | * | + | * Testis development |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008084 '''NM_008084'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008084 '''NM_008084'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 654: | Line 682: | ||
|- | |- | ||
|align="center"| Actb<ref name="ref10"/> | |align="center"| Actb<ref name="ref10"/> | ||
− | |align="center"| | + | |align="center"| β-Actin |
| | | | ||
− | * | + | * Testis development |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_007393 '''NM_007393'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_007393 '''NM_007393'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 666: | Line 694: | ||
|} | |} | ||
− | === | + | ===Molecular Types=== |
* mRNA | * mRNA | ||
Line 679: | Line 707: | ||
*'''Name''': Wen Zhen Lin | *'''Name''': Wen Zhen Lin | ||
*'''Email''': linwenzhen@msn.com | *'''Email''': linwenzhen@msn.com | ||
− | *''' | + | *'''Institution''': Department of Biochemistry and Molecular Biology, School of Preclinical Sciences, Guangxi Medical University, Nanning 530021, Guangxi, China |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=16514510807151339812&as_sdt=2005&sciodt=0,5&hl=en '''10'''] (Based on Google Scholar [2017-09-01]) | ||
− | =='''Intestinal Epithelial Cells'''== | + | =='''''Intestinal Epithelial Cells'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 695: | Line 725: | ||
|- | |- | ||
|align="center"| Ppib<ref name="ref11"/> | |align="center"| Ppib<ref name="ref11"/> | ||
− | |align="center"| Peptidylprolyl isomerase B | + | |align="center"| Peptidylprolyl isomerase (Cyclophilin) B |
| | | | ||
* Univeral reference gene in the differentiated fraction | * Univeral reference gene in the differentiated fraction | ||
Line 707: | Line 737: | ||
|- | |- | ||
|align="center"| Ppia<ref name="ref11"/> | |align="center"| Ppia<ref name="ref11"/> | ||
− | |align="center"| Peptidyl-prolyl cis–trans isomerase A | + | |align="center"| Peptidyl-prolyl cis–trans isomerase (Cyclophilin) A |
| | | | ||
− | * | + | * Proliferating intestinal compartments and IEC6 cells |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008907.1 '''NM_008907.1'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_008907.1 '''NM_008907.1'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 730: | Line 760: | ||
|align="center"| SYBR | |align="center"| SYBR | ||
|} | |} | ||
− | === | + | |
+ | ===Molecular Types=== | ||
* mRNA | * mRNA | ||
Line 740: | Line 771: | ||
*'''Name''': Maria Sirakov | *'''Name''': Maria Sirakov | ||
*'''Email''': maria.sirakov@ulb.ac.be | *'''Email''': maria.sirakov@ulb.ac.be | ||
− | *''' | + | *'''Institution''': Laboratoire de Génétique du Développement, Université Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires (IBMM), rue des Profs. Jeener et Brachet 12, 6041 Gosselies, Belgium |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=6555526528695445385&as_sdt=2005&sciodt=0,5&hl=en '''5'''] (Based on Google Scholar [2017-09-01]) | ||
− | ==''' | + | =='''''Skeletal Muscle & Different Genotypes'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 756: | Line 789: | ||
|- | |- | ||
|align="center"| Rpl27<ref name="ref12"/> | |align="center"| Rpl27<ref name="ref12"/> | ||
− | |align="center"| | + | |align="center"| Ribosomal protein L27 |
| | | | ||
*R129 strain | *R129 strain | ||
Line 770: | Line 803: | ||
|align="center"| Retention in endoplasmic reticulum 1 protein | |align="center"| Retention in endoplasmic reticulum 1 protein | ||
| | | | ||
− | *R129 strain | + | *R129 strain |
+ | *C57BL/6j strain | ||
+ | *C57BL/10 strain | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_026395 '''NM_026395'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_026395 '''NM_026395'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 780: | Line 815: | ||
|- | |- | ||
|align="center"| Rpl41<ref name="ref12"/> | |align="center"| Rpl41<ref name="ref12"/> | ||
− | |align="center"| | + | |align="center"| Ribosomal protein L41 |
| | | | ||
*R129 strain and Rpl7l1 | *R129 strain and Rpl7l1 | ||
− | *C57BL/6j | + | *C57BL/6j strain |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_018860 '''NM_018860'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_018860 '''NM_018860'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 793: | Line 828: | ||
|- | |- | ||
|align="center"| Rpl7l1<ref name="ref12"/> | |align="center"| Rpl7l1<ref name="ref12"/> | ||
− | |align="center"| | + | |align="center"| Ribosomal protein L7-like 1 |
| | | | ||
− | *C57BL/6j strain | + | *C57BL/6j strain |
+ | *C57BL/10 strain | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_025433 '''NM_025433'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_025433 '''NM_025433'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 805: | Line 841: | ||
|- | |- | ||
|align="center"| Aldoa<ref name="ref12"/> | |align="center"| Aldoa<ref name="ref12"/> | ||
− | |align="center"| | + | |align="center"| Aldolase A, fructose-bisphosphate |
| | | | ||
*C57BL/10 strain | *C57BL/10 strain | ||
Line 816: | Line 852: | ||
|align="center"| SYBR | |align="center"| SYBR | ||
|} | |} | ||
− | === | + | |
+ | ===Molecular Types=== | ||
* mRNA | * mRNA | ||
Line 825: | Line 862: | ||
*'''Name''': Kathryn N. North | *'''Name''': Kathryn N. North | ||
*'''Email''': Kathryn.north@mcri.edu.au | *'''Email''': Kathryn.north@mcri.edu.au | ||
− | *''' | + | *'''Institution''': Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=1062620161022216578&as_sdt=2005&sciodt=0,5&hl=en '''12'''] (Based on Google Scholar [2017-09-01]) | ||
=='''References'''== | =='''References'''== | ||
Line 876: | Line 915: | ||
Thomas K C, Zheng X F, Suarez F G, et al. Evidence based selection of commonly used RT-qPCR reference genes for the analysis of mouse skeletal muscle[J]. PloS one, 2014, 9(2): e88653. | Thomas K C, Zheng X F, Suarez F G, et al. Evidence based selection of commonly used RT-qPCR reference genes for the analysis of mouse skeletal muscle[J]. PloS one, 2014, 9(2): e88653. | ||
</ref> | </ref> | ||
+ | </references> | ||
+ | =='''Categories'''== | ||
+ | [[Category:Animals]] | ||
+ | [[Category:mRNA]] | ||
+ | [[Category:SYBR]] | ||
+ | [[Category:18S rRNA]] [[Category:18S rRNA]] [[Category:ACT]] [[Category:ACT]] [[Category:Aldoa]] [[Category:B2M]] [[Category:EF1α]] [[Category:GAPDH]] [[Category:GAPDH]] [[Category:GAPDH]] [[Category:GAPDH]] [[Category:GAPDH]] [[Category:GUSB]] [[Category:Histone]] [[Category:HK]] [[Category:HPRT]] [[Category:HPRT]] [[Category:HPRT]] [[Category:PGK]] [[Category:Cyclophilin]] [[Category:Rer1]] [[Category:RPL]] [[Category:SDHA]] [[Category:SPTBN]] [[Category:TBP]] [[Category:TBP]] [[Category:TBP]] [[Category:TGFB]] [[Category:Tubulin]] [[Category:WRNIP]] [[Category:YWHAZ]] [[Category:YWHAZ]][[Category:Different Tissues]] [[Category:Animal Model]] [[Category:Ageing]] [[Category:Lung Development]] [[Category:Specific Tissue]] [[Category:Specific Cells]] [[Category:Different Genotypes]] [[Category:Pathological conditions]] | ||
+ | {{Template:BackToTop}} | ||
− | + | [[Category:geNorm]] | |
+ | [[Category:NormFinder]] | ||
+ | [[Category:BestKeeper]] | ||
+ | [[Category:Delta Ct]] | ||
+ | [[Category:RefFinder]] |
Latest revision as of 07:41, 7 September 2017
Contents
- 1 Description
- 2 Dextran Sodium Sulfate Experimental Colitis
- 3 Mammary Glands
- 4 Uterus in the Peri-Implantation Period
- 5 Different Tissues & Ageing
- 6 Lung Development
- 7 Epithelial & Nonepithelial Cells
- 8 Preimplantation Embryos
- 9 Embryonic Stem Cells
- 10 Obesity Model
- 11 Testis Development
- 12 Intestinal Epithelial Cells
- 13 Skeletal Muscle & Different Genotypes
- 14 References
- 15 Categories
Description
- Mus musculus is a small mammal of the order Rodentia, characteristically having a pointed snout, small rounded ears, and a long naked or almost hairless tail. It is one of the most numerous species of the genus Mus. Although a wild animal, the house mouse mainly lives in association with humans (Modified from Wikipedia).
- Common Name: Mouse
- NCBI Taxonomy
Dextran Sodium Sulfate Experimental Colitis
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
Tbp[1] | TATA-box-binding protein |
|
NM_013684 |
|
86 | 60.8~61.3 | SYBR |
Eef2[1] | Eukaryotic Translation Elongation Factor 2 |
|
NM_007907.2 |
|
123 | 62.1~62.2 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Jean-Eric Ghia
- Email: Jean-Eric.Ghia@umanitoba.ca
- Institution: Immunology, University of Manitoba, Winnipeg, MB, Canada
Citation Statistics
Cited by 3 (Based on Google Scholar [2017-09-01])
Mammary Glands
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
HPRT[2] | Hypoxanthine guanine phosphoribosyl transferase |
|
NM_013556 |
|
86 | 60 | SYBR |
RPL13A[2] | Ribosomal protein L13A |
|
NM_009438 |
|
168 | 60 | SYBR |
GAPDH[2] | Glyceraldehyde-3-phosphate dehydrogenase |
|
NM_008084 |
|
150 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Y.L. Wang
- Email: ylwang2001@yahoo.cn
- Institution: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China
Citation Statistics
Cited by 11 (Based on Google Scholar [2017-09-01])
Uterus in the Peri-Implantation Period
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
RPLP0[3] | Ribosomal protein, large, P0 |
|
NM_007475 |
|
85 | 60 | SYBR |
GAPDH[3] | Glyceraldehydes-3-phosphate dehydrogenase |
|
NM_008084 |
|
186 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
Contact
- Name: AiHua Wang
- Email: pflin2001n@163.com
- Institution: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China
Citation Statistics
Cited by 28 (Based on Google Scholar [2017-09-01])
Different Tissues & Ageing
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
Rpl13a[4] | Ribosomal protein L13a |
|
NM_009438.5 |
|
96 | 60 | SYBR |
GAPDH[4] | Glyceraldehyde-3-phosphate dehydrogenase |
|
NM_008084.3 |
|
143 | 60 | SYBR |
Ppib[4] | Peptidylprolyl isomerase (Cyclophilin) B |
|
NM_011149.2 |
|
85 | 60 | SYBR |
Hprt[4] | Hypoxanthine guanine phosphoribosyl transferase |
|
NM_013556.2 |
|
121 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: B.D.Roussel
- Email: broussel@cyceron.fr
- Institution: INSERM, INSERM U919, Serine Proteases and Pathophysiology of the Neurovascular Unit, GIP CYCERON, University Caen Basse Normandie, boulevard Becquerel, 14074 Caen, France
Citation Statistics
Cited by 6 (Based on Google Scholar [2017-09-01])
Lung Development
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
Tuba1a[5] | Alpha 1a tubulin |
|
NM_011653 |
|
227 | 60.39~61.43 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Guillermo Barreto
- Email: guillermo.barreto@mpi-bn.mpg.de
- Institution: LOEWE Research Group Lung Cancer Epigenetic, Max-Planck-Institute for Heart and Lung Research, Parkstraße 1, 61231 Bad Nauheim, Germany
Citation Statistics
Cited by 7 (Based on Google Scholar [2017-09-01])
Epithelial & Nonepithelial Cells
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
GUSB[6] | Beta-glucuronidase |
|
NM_010368 |
|
197 | 55–60 | SYBR |
TBP[6] | TATA-box binding protein |
|
NM_008907 |
|
131 | 55–60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Yongping Su
- Email: suyp2003@yahoo.com.cn
- Institution: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Road, Shapingba District, Chongqing 400038, China
Citation Statistics
Cited by 55 (Based on Google Scholar [2017-09-01])
Preimplantation Embryos
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
h2afz[7] | H2A histone family, member Z |
|
NM_016750 |
|
89 | 60 | SYBR |
sptbn[7] | Pectrin beta, non-erythrocytic 1 |
|
NM_175836 |
|
101 | 55 | SYBR |
wrnip[7] | Werner helicase interacting protein 1 |
|
NM_030215 |
|
130 | 56 | SYBR |
ywhaz[7] | 14-3-3 protein zeta/delta (14-3-3ζ) |
|
NM_001253807 |
|
92 | 60 | SYBR |
tgfb1[7] | Transforming growth factor, beta 1 |
|
NM_011577 |
|
83 | 55 | SYBR |
18s[7] | 18S rRNA |
|
X00686 |
|
102 | 60 | SYBR |
actb[7] | β-actin |
|
NM_007393 |
|
98 | 56 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Jin-Hoi Kim
- Email: jhkim541@konkuk.ac.kr
- Institution: Department of Animal Biotechnology, KonKuk University, Seoul 143-701, Republic of Korea
Citation Statistics
Cited by 4 (Based on Google Scholar [2017-09-01])
Embryonic Stem Cells
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
Pgk1[8] | Phosphoglycerate kinase 1 |
|
NM_008828 |
|
110 | 60 | SYBR |
Sdha[8] | Succinate dehydrogenase complex, subunit A |
|
BC011301 |
|
134 | 60 | SYBR |
Tbp[8] | TATA box binding protein |
|
NM_013684 |
|
127 | 60 | SYBR |
Ywhaz[8] | Tyrosine 3-monooxygenase /tryptophan 5-monooxygenase activation protein, zeta polypeptide |
|
NM_013684 |
|
88 | 60 | SYBR |
Hk2[8] | Hexokinase 2 |
|
NM_011740 |
|
251 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
Contact
- Name: Michael C. Golding
- Email: mgolding@cvm.tamu.edu
- Institution: College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, United States of America
Citation Statistics
Cited by 42 (Based on Google Scholar [2017-09-01])
Obesity Model
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
B2M[9] | Beta 2 Microglobulin |
|
NM_009735 |
|
103 | 83.5 | SYBR |
18S[9] | 18S Ribosomal RNA |
|
NR_003278 |
|
133 | 85 | SYBR |
HPRT1[9] | Hypoxanthine phosphoribosyl transferase |
|
NM_013556 |
|
167 | 81.5 | SYBR |
RPlP0[9] | Ribosomal Protein large P0 |
|
NM_007475 |
|
136 | 87.5 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
Contact
- Name: Petra Matoušková
- Email: matousp7@faf.cuni.cz
- Institution: Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic
Citation Statistics
Cited by 26 (Based on Google Scholar [2017-09-01])
Testis Development
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
Ppia[10] | Peptidylprolyl isomerase (Cyclophilin) A |
|
NM_008907 |
|
144 | 60 | SYBR |
Gapdh[10] | Glyceraldehyde-3-phosphate dehydrogenase |
|
NM_008084 |
|
150 | 60 | SYBR |
Actb[10] | β-Actin |
|
NM_007393 |
|
171 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- Comparative ΔCt method && Related Reference
- BestKeeper method && Related Reference
- Ref-Finder method && Related Reference
Contact
- Name: Wen Zhen Lin
- Email: linwenzhen@msn.com
- Institution: Department of Biochemistry and Molecular Biology, School of Preclinical Sciences, Guangxi Medical University, Nanning 530021, Guangxi, China
Citation Statistics
Cited by 10 (Based on Google Scholar [2017-09-01])
Intestinal Epithelial Cells
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
Ppib[11] | Peptidylprolyl isomerase (Cyclophilin) B |
|
NM_011149.2 |
|
156 | 60 | SYBR |
Ppia[11] | Peptidyl-prolyl cis–trans isomerase (Cyclophilin) A |
|
NM_008907.1 |
|
109 | 60 | SYBR |
Gapdh[11] | Glyceraldehyde-3-phosphate dehydrogenase |
|
XM_003945995.1 |
|
152 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Maria Sirakov
- Email: maria.sirakov@ulb.ac.be
- Institution: Laboratoire de Génétique du Développement, Université Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires (IBMM), rue des Profs. Jeener et Brachet 12, 6041 Gosselies, Belgium
Citation Statistics
Cited by 5 (Based on Google Scholar [2017-09-01])
Skeletal Muscle & Different Genotypes
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
Rpl27[12] | Ribosomal protein L27 |
|
NM_011289 |
|
143 | 65 | SYBR |
Rer1[12] | Retention in endoplasmic reticulum 1 protein |
|
NM_026395 |
|
137 | 62 | SYBR |
Rpl41[12] | Ribosomal protein L41 |
|
NM_018860 |
|
113 | 65 | SYBR |
Rpl7l1[12] | Ribosomal protein L7-like 1 |
|
NM_025433 |
|
110 | 65 | SYBR |
Aldoa[12] | Aldolase A, fructose-bisphosphate |
|
NM_001177307 |
|
136 | 62 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- Ref-Finder method && Related Reference
Contact
- Name: Kathryn N. North
- Email: Kathryn.north@mcri.edu.au
- Institution: Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic
Citation Statistics
Cited by 12 (Based on Google Scholar [2017-09-01])
References
- ↑ 1.0 1.1 Eissa N, Hussein H, Wang H, et al. Stability of Reference Genes for Messenger RNA Quantification by Real-Time PCR in Mouse Dextran Sodium Sulfate Experimental Colitis[J]. PloS one, 2016, 11(5): e0156289.
- ↑ 2.0 2.1 2.2 Han L Q, Yang G Y, Zhu H S, et al. Selection and use of reference genes in mouse mammary glands[J]. Genet Mol Res, 2010, 9(1): 449-56.
- ↑ 3.0 3.1 Lin P F, Lan X L, Chen F L, et al. Reference gene selection for real-time quantitative PCR analysis of the mouse uterus in the peri-implantation period[J]. PloS one, 2013, 8(4): e62462.
- ↑ 4.0 4.1 4.2 4.3 Bruckert G, Vivien D, Docagne F, et al. Normalization of Reverse Transcription Quantitative PCR Data During Ageing in Distinct Cerebral Structures[J]. Molecular neurobiology, 2016, 53(3): 1540-1550.
- ↑ Mehta A, Dobersch S, Dammann R H, et al. Validation of Tuba1a as appropriate internal control for normalization of gene expression analysis during mouse lung development[J]. International journal of molecular sciences, 2015, 16(3): 4492-4511.
- ↑ 6.0 6.1 Wang F, Wang J, Liu D, et al. Normalizing genes for real-time polymerase chain reaction in epithelial and nonepithelial cells of mouse small intestine[J]. Analytical biochemistry, 2010, 399(2): 211-217.
- ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 Jeong J K, Kang M H, Gurunathan S, et al. Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real-time quantitative RT-PCR (RT-qPCR)[J]. BMC research notes, 2014, 7(1): 675.
- ↑ 8.0 8.1 8.2 8.3 8.4 Veazey K J, Golding M C. Selection of stable reference genes for quantitative rt-PCR comparisons of mouse embryonic and extra-embryonic stem cells[J]. PloS one, 2011, 6(11): e27592.
- ↑ 9.0 9.1 9.2 9.3 Matoušková P, Bártíková H, Boušová I, et al. Reference genes for real-time PCR quantification of messenger RNAs and microRNAs in mouse model of obesity[J]. PLoS One, 2014, 9(1): e86033.
- ↑ 10.0 10.1 10.2 Gong Z K, Wang S J, Huang Y Q, et al. Identification and validation of suitable reference genes for RT-qPCR analysis in mouse testis development[J]. Molecular genetics and genomics, 2014, 289(6): 1157-1169.
- ↑ 11.0 11.1 11.2 Sirakov M, Borra M, Cambuli F M, et al. Defining suitable reference genes for RT-qPCR analysis on intestinal epithelial cells[J]. Molecular biotechnology, 2013, 54(3): 930-938.
- ↑ 12.0 12.1 12.2 12.3 12.4 Thomas K C, Zheng X F, Suarez F G, et al. Evidence based selection of commonly used RT-qPCR reference genes for the analysis of mouse skeletal muscle[J]. PloS one, 2014, 9(2): e88653.
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