Mus musculus

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Contents

Mouse Dextran Sodium Sulfate Experimental Colitis

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
Tbp[1] TATA-box-binding protein
  • Mouse Dextran Sodium Sulfate Experimental Colitis
 NM_013684
  • F:ACCGTGAATCTTGGCTGTAAAC
  • R:GCAGCAAATCGCTTGGGATTA
 86 60.8~61.3 SYBR
Eef2[1] Eukaryotic Translation Elongation Factor 2
  • Mouse Dextran Sodium Sulfate Experimental Colitis
 NM_007907.2
  • F:TGTCAGTCATCGCCCATGTG
  • R:CATCCTTGCGAGTGTCAGTGA
 123 62.1~62.2 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Jean-Eric Ghia
  • Email: Jean-Eric.Ghia@umanitoba.ca
  • Institute: Immunology, University of Manitoba, Winnipeg, MB, Canada

Citation Statistics

Cited by 3 (Based on Google Scholar [2017-06-16])

Mouse Mammary Glands

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
HPRT[2] hypoxanthine guanine phosphoribosyl transferase
  • Mammary glands
 NM_013556
  • F:CCTAAGATGAGCGCAAGTTGAA
  • R:CCACAGGACTAGAACACCTGCTAA
 86 60 SYBR
RPL13A[2] ribosomal protein L13A
  • Mammary glands
 NM_009438
  • F:CCTAAGATGAGCGCAAGTTGAA
  • R:CCACAGGACTAGAACACCTGCTAA
 168 60 SYBR
GAPDH[2] glyceraldehyde-3-phosphate dehydrogenase
  • Mammary glands
 NM_008084
  • F:TGTGTCCGTCGTGGATCTGA
  • R:TTGCTGTTGAAGTCGCAGGAG
 150 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Y.L. Wang
  • Email: ylwang2001@yahoo.cn
  • Institute: College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China

Citation Statistics

Cited by 11 (Based on Google Scholar [2017-06-16])

Mouse Uterus in the Peri-Implantation Period

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
RPLP0[3] Ribosomal protein, large, P0
  • Early pregnancy, pseudopregnancy, delayed implantation
  • Activation, artificial decidualization
  • Hormonal treatment model
 NM_007475
  • F:GGACCCGAGAAGACCTCCTT
  • R:GCACATCACTCAGAATTTCAATGG
 85 60 SYBR
GAPDH[3] Glyceraldehydes-3-phosphate dehydrogenase
  • Early pregnancy, pseudopregnancy, delayed implantation
  • Activation, artificial decidualization
  • Hormonal treatment model
 NM_008084
  • F:TCACTGCCACCCAGAAGA
  • R:GACGGACACATTGGGGGTAG
 186 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: AiHua Wang
  • Email: pflin2001n@163.com
  • Institute: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China

Citation Statistics

Cited by 28 (Based on Google Scholar [2017-06-16])

During Ageing in Distinct Cerebral Structures

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
Rpl13a[4] ribosomal protein L13a
  • Cerebral cortex during ageing
  • Cortex during ageing
  • Hippocampus during ageing
 NM_009438.5
  • F:tacgctgtgaaggcatcaac
  • R:gggaggggttggtattcatc
 96 60 SYBR
GAPDH[4] glyceraldehyde-3-phosphate dehydrogenase
  • Cerebral cortex during ageing
  • Cortex during ageing
  • Cerebellum during ageing
 NM_008084.3
  • F:tgcgacttcaacagcaactc
  • R:atgtaggcaatgaggtccac
 143 60 SYBR
Ppib[4] peptidylprolyl isomerase B
  • Striatum during ageing
  • Cerebellum during ageing
 NM_011149.2
  • F:cagcaagttccatcgtgtca
  • R:gatgctctttcctcctgtgc
 85 60 SYBR
Hprt[4] hypoxanthine guanine phosphoribosyl transferase
  • Hippocampus during ageing
  • Cerebellum during ageing
 NM_013556.2
  • F:ctttgctgacctgctggatt
  • R:tatgtcccccgttgactgat
 121 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: B.D.Roussel
  • Email: broussel@cyceron.fr
  • Institute: INSERM, INSERM U919, Serine Proteases and Pathophysiology of the Neurovascular Unit, GIP CYCERON, University Caen Basse Normandie, boulevard Becquerel, 14074 Caen, France

Citation Statistics

Cited by 4 (Based on Google Scholar [2017-06-16])

During Mouse Lung Development

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
Tuba1a[5] alpha 1a tubulin
  • During different stages of lung development
 NM_011653
  • F:CCGCGAAGCAGCAACCAT
  • R:CCAGGTCTACGAACACTGCC
 227 60.39~61.43 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Guillermo Barreto
  • Email: guillermo.barreto@mpi-bn.mpg.de
  • Institute: LOEWE Research Group Lung Cancer Epigenetic, Max-Planck-Institute for Heart and Lung Research, Parkstraße 1, 61231 Bad Nauheim, Germany

Citation Statistics

Cited by 7 (Based on Google Scholar [2017-06-16])

Epithelial and Nonepithelial Cells of Small Intestine

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
GUSB[6] Beta-glucuronidase
  • Mouse small intestine
 NM_010368
  • F:CCGATTATCCAGAGCGAGTATG
  • R:CTCAGCGGTGACTGGTTCG
 197 55–60 SYBR
TBP[6] TATAA-box binding protein
  • Mouse small intestine
 NM_008907
  • F:CAAACCCAGAATTGTTCTCCTT
  • R:ATGTGGTCTTCCTGAATCCCT
 131 55–60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Yongping Su
  • Email: suyp2003@yahoo.com.cn
  • Institute: Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Third Military Medical University, 30 Gaotanyan Road, Shapingba District, Chongqing 400038, China

Citation Statistics

Cited by 52 (Based on Google Scholar [2017-06-16])

Preimplantation Embryos

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
h2afz[7] H2A histone family, member Z
  • B6D2F-1 and C57BL/6 strains
  • Blastocyst embryonic stages cultured in KSOM medium
  • KOSOM and CZB cultures of B6D2F-1 and C57BL/6 strain-derived embryos
  • Zygote stage embryos
  • 2-cell, 4-cell, 8-cell stage embryos cultured in M16 medium
 NM_016750
  • F:GTGGACTGTATCTCTGTGAA
  • R:GGTTGGTTGGAAGGCTAA
 89 60 SYBR
sptbn[7] pectrin beta, non-erythrocytic 1
  • ICR strain-derived embryos
 NM_175836
  • F:TCTAATGGTTACTTGCTTGT
  • R:CAATAGTTACAGTGACAGAGA
 101 55 SYBR
wrnip[7] Werner helicase interacting protein 1
  • M16 culture of B6D2F-1
  • C57BL/6 strain-derived embryos
  • 2-cell, 4-cell stage embryos cultured in CZB medium
  • Blastocyst stage embryos cultured in M16 medium
 NM_030215
  • F:ATGAGTAGGATGCTTGTA
  • R:TAACCACCTCCATCTATG
 130 56 SYBR
ywhaz[7] ywhaz
  • Zygote & molular embryonic stages cultured in KSOM medium
  • Molular stage embryos cultured in CZB medium
 NM_001253807
  • F:CAGTAGATGGAGAAAGATTTGC
  • R:GGGACAATTAGGGAAGTAAGT
 92 60 SYBR
tgfb1[7] transforming growth factor, beta 1
  • 2-cell embryonic stages cultured in KSOM medium
 NM_011577
  • F:TATACTGAGACACCTTGG
  • R:GTGATAGTCCTGAATAATTTG
 83 55 SYBR
18s[7] 18S rRNA
  • 4-cell, 8-cell embryonic stages cultured in KSOM medium
  • Zygote embryos cultured in M16 medium
 X00686
  • F:CGCCGCTAGAGGTGAAATTCT
  • R:CGAACCTCCGACTTTCGTTCT
 102 60 SYBR
actb[7] actin, beta
  • 8-cell stage embryos cultured in M16 medium
 NM_007393
  • F:ATCTTCCGCCTTAATACT
  • R:GCCTTCATACATCAAGTT
 98 56 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Jin-Hoi Kim
  • Email: jhkim541@konkuk.ac.kr
  • Institute: Department of Animal Biotechnology, KonKuk University, Seoul 143-701, Republic of Korea

Citation Statistics

Cited by 4 (Based on Google Scholar [2017-06-16])

Embryonic and Extra Embryonic Stem Cells

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
Pgk1[8] phosphoglycerate kinase 1
  • Between ES, TS and XEN stem cells
  • The in vitro differentiation of embryonic and trophectoderm stem cells
 NM_008828
  • F:CTGACTTTGGACAAGCTGGACG
  • R:GCAGCCTTGATCCTTTGGTTG
 110 60 SYBR
Sdha[8] Succinate dehydrogenase complex, subunit A
  • Between ES, TS and XEN stem cells
  • The in vitro differentiation of embryonic and trophectoderm stem cells
 BC011301
  • F:GCTCCTGCCTCTGTGGTTGA
  • R:AGCAACACCGATGAGCCTG
 134 60 SYBR
Tbp[8] TATA box binding protein
  • Between ES, TS and XEN stem cells
  • The in vitro differentiation of embryonic and trophectoderm stem cells
 NM_013684
  • F:GAAGAACAATCCAGACTAGCAGCA
  • R:CCTTATAGGGAACTTCACATCACAG
 127 60 SYBR
Ywhaz[8] Tyrosine 3-monooxygenase /tryptophan 5-monooxygenase activation protein, zeta polypeptide
  • The in vitro differentiation of embryonic and trophectoderm stem cells
 NM_013684
  • F:TTGATCCCCAATGCTTCGC
  • R:CAGCAACCTCGGCCAAGTAA
 88 60 SYBR
Hk2[8] hexokinase 2
  • The in vitro differentiation of embryonic and trophectoderm stem cells
 NM_011740
  • F:CCCTGTGAAGATGTTGCCCAC
  • R:TGCCCATGTACTCAAGGAAGT
 251 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Michael C. Golding
  • Email: mgolding@cvm.tamu.edu
  • Institute: College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, United States of America

Citation Statistics

Cited by 41 (Based on Google Scholar [2017-06-16])

Mouse Model of Obesity

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
B2M[9] Beta 2 Microglobulin
  • Liver of monosodium glutamate (MSG)-injected mice with developed central obesity
 NM_009735
  • F:GGTCTTTCTGGTGCTTGTCTCA
  • R:GTTCGGCTTCCCATTCTCC
 103 83.5 SYBR
18S[9] 18S Ribosomal RNA
  • Liver of monosodium glutamate (MSG)-injected mice with developed central obesity
  • Small intestine of monosodium glutamate (MSG)-injected mice with developed central obesity
 NR_003278
  • F:GGCCGTTCTTAGTTGGTGGAGCG
  • R:CTGAACGCCACTTGTCCCTC
 133 85 SYBR
HPRT1[9] Hypoxanthine phosphoribosyl transferase
  • Liver of monosodium glutamate (MSG)-injected mice with developed central obesity
  • Small intestine of monosodium glutamate (MSG)-injected mice with developed central obesity
 NM_013556
  • F:CAGTCCCAGCGTCGTGATTA
  • R:GGCCTCCCATCTCCTTCATG
 167 81.5 SYBR
RPlP0[9] Ribosomal Protein large P0
  • Small intestine of monosodium glutamate (MSG)-injected mice with developed central obesity
 NM_007475
  • F:GATGGGCAACTGTACCTGACTG
  • R:CTGGGCTCCTCTTGGAATG
 136 87.5 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Petra Matoušková
  • Email: matousp7@faf.cuni.cz
  • Institute: Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic

Citation Statistics

Cited by 23 (Based on Google Scholar [2017-06-16])

Testis Development

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
Ppia[10] peptidylprolyl isomerase A
  • Testis development
 NM_008907
  • F:TGTGCCAGGGTGGTGACTT
  • R:CGTTTGTGTTTGGTCCAGCAT
 144 60 SYBR
Gapdh[10] glyceraldehyde-3-phosphate dehydrogenase
  • Testis development
 NM_008084
  • F:TGTGTCCGTCGTGGATCTGA
  • R:TTGCTGTTGAAGTCGCAGGAG
 150 60 SYBR
Actb[10] beta actin
  • Testis development
 NM_007393
  • F:CATCCGTAAAGACCTCTATGCCAAC
  • R:ATGGAGCCACCGATCCACA
 171 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Wen Zhen Lin
  • Email: linwenzhen@msn.com
  • Institute: Department of Biochemistry and Molecular Biology, School of Preclinical Sciences, Guangxi Medical University, Nanning 530021, Guangxi, China

Citation Statistics

Cited by 8 (Based on Google Scholar [2017-06-16])

Intestinal Epithelial Cells

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
Ppib[11] Peptidylprolyl isomerase B
  • Univeral reference gene in the differentiated fraction
 NM_011149.2
  • F:CACCAATGGCTCACAGTTCTT
  • R:ATGACATCCTTCAGTGGCTTG
 156 60 SYBR
Ppia[11] Peptidyl-prolyl cis–trans isomerase A
  • proliferating intestinal compartments and IEC6 cells
 NM_008907.1
  • F:CCACTGTCGCTTTTCGCCGC
  • R:TGCAAACAGCTCGAAGGAGACGC
 109 60 SYBR
Gapdh[11] Glyceraldehyde-3-phosphate dehydrogenase
  • Univeral reference gene in the differentiated fraction
 XM_003945995.1
  • F:AGCAAGGACACTGAGCAAGAG
  • R:AGCAAGGACACTGAGCAAGAG
 152 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Maria Sirakov
  • Email: maria.sirakov@ulb.ac.be
  • Institute: Laboratoire de Génétique du Développement, Université Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires (IBMM), rue des Profs. Jeener et Brachet 12, 6041 Gosselies, Belgium

Citation Statistics

Cited by 4 (Based on Google Scholar [2017-06-16])

Mouse Skeletal Muscle

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
Rpl27[12] ribosomal protein L27
  • R129 strain
 NM_011289
  • F:AAGCCGTCATCGTGAAGAACA
  • R:CTTGATCTTGGATCGCTTGGC
 143 65 SYBR
Rer1[12] Retention in endoplasmic reticulum 1 protein
  • R129 strain & C57BL/6j strain & C57BL/10 strain
 NM_026395
  • F:GCCTTGGGAATTTACCACCT
  • R:CTTCGAATGAAGGGACGAAA
 137 62 SYBR
Rpl41[12] ribosomal protein L41
  • R129 strain and Rpl7l1
  • C57BL/6j
 NM_018860
  • F:GCCATGAGAGCGAAGTGG
  • R:CTCCTGCAGGCGTCGTAG
 113 65 SYBR
Rpl7l1[12] ribosomal protein L7-like 1
  • C57BL/6j strain & C57BL/10 strain
 NM_025433
  • F:ACGGTGGAGCCTTATGTGAC
  • R:TCCGTCAGAGGGACTGTCTT
 110 65 SYBR
Aldoa[12] aldolase A, fructose-bisphosphate
  • C57BL/10 strain
 NM_001177307
  • F:ACATTGCTGAAGCCCAACAT
  • R:ACAGGAAAGTGACCCCAGTG
 136 62 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Kathryn N. North
  • Email: Kathryn.north@mcri.edu.au
  • Institute: Department of Biochemical Sciences, Charles University in Prague, Faculty of Pharmacy, Hradec Králové, Czech Republic

Citation Statistics

Cited by 11 (Based on Google Scholar [2017-06-16])

References

  1. ↑ 1.0 1.1 Eissa N, Hussein H, Wang H, et al. Stability of Reference Genes for Messenger RNA Quantification by Real-Time PCR in Mouse Dextran Sodium Sulfate Experimental Colitis[J]. PloS one, 2016, 11(5): e0156289.
  2. ↑ 2.0 2.1 2.2 Han L Q, Yang G Y, Zhu H S, et al. Selection and use of reference genes in mouse mammary glands[J]. Genet Mol Res, 2010, 9(1): 449-56.
  3. ↑ 3.0 3.1 Lin P F, Lan X L, Chen F L, et al. Reference gene selection for real-time quantitative PCR analysis of the mouse uterus in the peri-implantation period[J]. PloS one, 2013, 8(4): e62462.
  4. ↑ 4.0 4.1 4.2 4.3 Bruckert G, Vivien D, Docagne F, et al. Normalization of Reverse Transcription Quantitative PCR Data During Ageing in Distinct Cerebral Structures[J]. Molecular neurobiology, 2016, 53(3): 1540-1550.
  5. ↑ Mehta A, Dobersch S, Dammann R H, et al. Validation of Tuba1a as appropriate internal control for normalization of gene expression analysis during mouse lung development[J]. International journal of molecular sciences, 2015, 16(3): 4492-4511.
  6. ↑ 6.0 6.1 Wang F, Wang J, Liu D, et al. Normalizing genes for real-time polymerase chain reaction in epithelial and nonepithelial cells of mouse small intestine[J]. Analytical biochemistry, 2010, 399(2): 211-217.
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 Jeong J K, Kang M H, Gurunathan S, et al. Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real-time quantitative RT-PCR (RT-qPCR)[J]. BMC research notes, 2014, 7(1): 675.
  8. ↑ 8.0 8.1 8.2 8.3 8.4 Veazey K J, Golding M C. Selection of stable reference genes for quantitative rt-PCR comparisons of mouse embryonic and extra-embryonic stem cells[J]. PloS one, 2011, 6(11): e27592.
  9. ↑ 9.0 9.1 9.2 9.3 Matoušková P, Bártíková H, Boušová I, et al. Reference genes for real-time PCR quantification of messenger RNAs and microRNAs in mouse model of obesity[J]. PLoS One, 2014, 9(1): e86033.
  10. ↑ 10.0 10.1 10.2 Gong Z K, Wang S J, Huang Y Q, et al. Identification and validation of suitable reference genes for RT-qPCR analysis in mouse testis development[J]. Molecular genetics and genomics, 2014, 289(6): 1157-1169.
  11. ↑ 11.0 11.1 11.2 Sirakov M, Borra M, Cambuli F M, et al. Defining suitable reference genes for RT-qPCR analysis on intestinal epithelial cells[J]. Molecular biotechnology, 2013, 54(3): 930-938.
  12. ↑ 12.0 12.1 12.2 12.3 12.4 Thomas K C, Zheng X F, Suarez F G, et al. Evidence based selection of commonly used RT-qPCR reference genes for the analysis of mouse skeletal muscle[J]. PloS one, 2014, 9(2): e88653.
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