Neurospora crassa
Contents
Description
- Neurospora crassa is a filamentous fungus classified under the phylum Ascomycota and is widely distributed in nature. It is typically found growing on vegetation killed by fire because it is able to synthesize and secrete high levels of enzyme types involved in lignocellulosic biomass degradation. It is also able to convert pentose sugars, cellulose polymers, and agro-industrial residues to ethanol. It has served as a model organism for studying circadian pathways and has gained attention from the biofuel industry due to its enhanced capacity for cellulase production[1][2].
- Common Name: NA
- NCBI Taxonomy
Different Environmental Conditions
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
btl[1] | Beta-tubulin |
|
NCU04054 |
|
103 | 83.21 | SYBR |
asl[1] | ATP synthase lyase gamma subunit |
|
NCU09119.7 |
|
114 | 81.35 | SYBR |
vma1[1] | Vacuolar ATPase subunit 1 |
|
J03955.1 |
|
111 | 80.45 | SYBR |
tbp[1] | TATA binding box protein |
|
NCU044770 |
|
136 | 79.38 | SYBR |
vma2[1] | Vacuolar ATPase subunit 2 |
|
J03956.1 |
|
127 | 78.90 | SYBR |
ACT[1] | Beta-actin |
|
NCU04173 |
|
110 | 79.10 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Kathleen D. Cusick
- Email: Kathleen_cusick.ctr@code6100.nrl.navy.mil
- Institution: National Research Council Associateship, U.S. Naval Research Laboratory, Washington, District of Columbia, United States of America
Citation Statistics
Cited by 7 (Based on Google Scholar [2017-09-01])
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Cusick KD, Fitzgerald LA, Pirlo RK, et al. (2014) Selection and evaluation of reference genes for expression studies with quantitative PCR in the model fungus Neurospora crassa under different environmental conditions in continuous culture. PLoS One 9, e112706
- ↑ Baker CL, Loros JJ, Dunlap JC (2012) The circadian clock of Neurospora crassa. Fems Microbiology Reviews 36, 95-110.