Difference between revisions of "Octopus vulgaris"

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==Description==
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=='''Description'''==
[[File:REFqPCR200902.jpg|right|127px|]]
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[[File:Octopus vulgaris.png|right|200px|link=Octopus vulgaris]]
* Octopus vulgaris is the most important octopus species in worldwide fisheries and represents a major protein resource in most fish-eating countries. It is of great commercial importance in Mediterranean, South American and Asian countries as well as in the NW Atlantic coasts of Spain and Portugal.<ref name="ref1"/> <ref name="ref2"/>.
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* '''''Octopus vulgaris''''' is the most important octopus species in worldwide fisheries and represents a major protein resource in most fish-eating countries. It is of great commercial importance in Mediterranean, South American and Asian countries as well as in the NW Atlantic coasts of Spain and Portugal<ref name="ref1"/><ref name="ref2"/>.
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* <font color=blue>'''Common Name:'''</font> '''Common octopus'''
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* [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=6645 <font color=blue>'''NCBI Taxonomy'''</font>]
  
 
=='''''Brain'''''==
 
=='''''Brain'''''==
===Reference Genes===
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===Internal Control Genes===
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
|-
 
|-
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! style="width=25% font-size:9pt "|Application Scope  
 
! style="width=25% font-size:9pt "|Application Scope  
 
! Accession Number  
 
! Accession Number  
! Primer
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! Primers (5'-3')<br>[Forward/Reverse]
 
! Size [bp]  
 
! Size [bp]  
 
! Tm [℃]
 
! Tm [℃]
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|-
 
|-
 
|align="center"| ubi<ref name="ref1"/>
 
|align="center"| ubi<ref name="ref1"/>
|align="center"| ubiquitin
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|align="center"| Ubiquitin
 
|
 
|
* Universal reference gene
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* Brain
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/224037718 '''FJ617440''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/224037718 '''FJ617440''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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|align="center"| α-tubulin
 
|align="center"| α-tubulin
 
|
 
|
* Universal reference gene
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* Brain
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/X15845 '''X15845''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/X15845 '''X15845''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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|}
 
|}
  
===Moleculer Types===
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===Molecular Types===
 
*mRNA
 
*mRNA
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===Evaluation Methods===  
 
===Evaluation Methods===  
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
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*'''Institution''': Laboratorio di Neurobiologia, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Napoli, Italy
 
*'''Institution''': Laboratorio di Neurobiologia, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Napoli, Italy
  
==Citation Statistics==
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===Citation Statistics===
Cited by '''19''' (Based on Google Scholar [2017-06-01])
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Cited by [https://scholar.google.com/scholar?cites=7404353325846467532&as_sdt=2005&sciodt=0,5&hl=en '''20'''] (Based on Google Scholar [2017-09-01])
  
 
=='''References'''==
 
=='''References'''==
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</ref>
 
</ref>
 
</references>
 
</references>
 
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=='''Categories'''==
 
[[Category:Animals]]
 
[[Category:Animals]]
 
[[Category:mRNA]]
 
[[Category:mRNA]]
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[[Category:SYBR]]
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[[Category:Tubulin]] [[Category:Ubiquitin]][[Category:Specific Tissue]]  [[Category:Brain Tissue]]
  
[[Category:SYBR]]
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[[Category:geNorm]]
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[[Category:NormFinder]]
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[[Category:BestKeeper]]

Latest revision as of 04:22, 1 September 2017

Description

Octopus vulgaris.png
  • Octopus vulgaris is the most important octopus species in worldwide fisheries and represents a major protein resource in most fish-eating countries. It is of great commercial importance in Mediterranean, South American and Asian countries as well as in the NW Atlantic coasts of Spain and Portugal[1][2].
  • Common Name: Common octopus
  • NCBI Taxonomy

Brain

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
ubi[1] Ubiquitin
  • Brain
FJ617440
  • F:TCAAAACCGCCAACTTAACC
  • R:CCTTCATTTGGTCCTTCGTC
113 60 SYBR
tubA[1] α-tubulin
  • Brain
X15845
  • F:ACTGGTGTCCAACTGGCTTC
  • R:TGCTTAACATGCACACAGCA
105 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Graziano Fiorito
  • Email: gfiorito@szn.it
  • Institution: Laboratorio di Neurobiologia, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Napoli, Italy

Citation Statistics

Cited by 20 (Based on Google Scholar [2017-09-01])

References

  1. 1.0 1.1 1.2 Sirakov M, Zarrella I, Borra M, Rizzo F, Biffali E, Arnone MI, Fiorito G. Selection and validation of a set of reliable reference genes for quantitative RT-PCR studies in the brain of the Cephalopod Mollusc Octopus vulgaris. BMC Mol Biol. 2009 Jul 14;10:70. doi: 10.1186/1471-2199-10-70. PubMed PMID: 19602224; PubMed Central PMCID: PMC2722649.
  2. Castellanos-Martínez S, Arteta D, Catarino S, Gestal C. De novo transcriptome sequencing of the Octopus vulgaris hemocytes using Illumina RNA-Seq technology: response to the infection by the gastrointestinal parasite Aggregata octopiana. PLoS One. 2014 Oct 16;9(10):e107873. doi: 10.1371/journal.pone.0107873. eCollection 2014. PubMed PMID: 25329466; PubMed Central PMCID: PMC4199593.

Categories