View source for Oreochromis niloticus

=='''Description'''==

=='''''Different Tissues & Infection'''==
===Reference Genes===
{|class="wikitable sortable" style="font-size:10pt; width:100%"
|-
! Gene Symbol  
! Gene Name 
! style="width=25%　font-size:9pt　"|Application Scope 
! Accession Number 
! Primer 
! Size [bp] 
! Tm [℃]
! Detection
|-
|align="center"| UBCE<ref name="ref1"/>
|align="center"| Ubiquitin-conjugating enzyme 
|
*in the intestine, liver, brain, kidney, and spleen after S. iniae infection, and in the liver, kidney, and spleen after S. agalactiae infection
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/XM_003460024 '''XM_003460024'''] 
|nowrap style="font-size:9pt"|
* F:CTCTCAAATCAATGCCACTTCC
* R:CCCTGGTGGAGGTTCCTTGT
|align="center"| 130
|align="center"| 59
|align="center"| SYBR
|-
|align="center"| EF1A<ref name="ref1"/>
|align="center"| EF-1a mRNA for elongation factor 1a 
|
*heart and muscle after S. iniae or S. agalactiae infection
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AB075952 '''AB075952'''] 
|nowrap style="font-size:9pt"|
* F:GCACGCTCTGCTGGCCTTT
* R:GCGCTCAATCTTCCATCCC
|align="center"| 250
|align="center"| 59
|align="center"| SYBR
|-
|align="center"| GADPH<ref name="ref1"/>
|align="center"| Glyceraldehyde-3-phosphate dehydrogenase
|
*in intestine and brain after S. agalactiae infection
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/JN381952 '''JN381952'''] 
|nowrap style="font-size:9pt"|
* F:GATAATGGCAAACTTGTCGTCG
* R:ACATTGGAGCATCGGGTGAG
|align="center"| 205
|align="center"| 59
|align="center"| SYBR
|-
|align="center"|  18S rRNA<ref name="ref1"/>
|align="center"| 18S ribosomal RNA 
|
*across the various tissues
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/JF698683 '''JF698683'''] 
|nowrap style="font-size:9pt"|
* F:GGACACGGAAAGGATTGACAG
* R:GTTCGTTATCGGAATTAACCAGAC
|align="center"| 111
|align="center"| 59
|align="center"| SYBR
|}
===Moleculer Type===
* mRNA
===Evaluation Methods===
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
*  '''Ref-Finder method''' && [https://www.ncbi.nlm.nih.gov/pubmed/22290409 '''Related Reference''']
===Contact===
*'''Name''': Hua Wen
*'''Email''': wenhua.hb@163.com
*'''Institution''': Key Laboratory of Freshwater Biodiversity Conservation and Utilization of Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China
=='''References'''==
<references>
<ref name="ref1">
Yang C G, Wang X L, Tian J, et al. Evaluation of reference genes for quantitative real-time RT-PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus)[J]. Gene, 2013, 527(1): 183-192.
</ref>
</references>