Difference between revisions of "Oreochromis niloticus"
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Yang C G, Wang X L, Tian J, et al. Evaluation of reference genes for quantitative real-time RT-PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus)[J]. Gene, 2013, 527(1): 183-192. | Yang C G, Wang X L, Tian J, et al. Evaluation of reference genes for quantitative real-time RT-PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus)[J]. Gene, 2013, 527(1): 183-192. | ||
| + | </ref> | ||
| + | <ref name="ref2"> | ||
| + | Ijiri S, Kaneko H, Kobayashi T, Wang DS, Sakai F, Paul-Prasanth B, Nakamura M, | ||
| + | Nagahama Y. Sexual dimorphic expression of genes in gonads during early | ||
| + | differentiation of a teleost fish, the Nile tilapia Oreochromis niloticus. Biol | ||
| + | Reprod. 2008 Feb;78(2):333-41. Epub 2007 Oct 17. PubMed PMID: 17942796. | ||
</ref> | </ref> | ||
</references> | </references> | ||
Revision as of 03:24, 17 June 2017
Contents
Description
- The Nile tilapia (Oreochromis niloticus) is a species of tilapia, a cichlid fish native to Africa from Egypt south to east and central Africa, and as far west as Gambia.
- It is also native to Israel, and numerous introduced populations exist outside its natural range (e.g., Brazil).[2] It is also commercially known as mango fish, nilotica, or boulti.
- The Nile tilapia, a gonochoristic teleost fish with an XX/XY sex-determining system, provides an excellent model for studying gonadal sex differentiation because genetic all-females and all-males are available[1][2].
Different Tissues & Infection
Reference Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primer | Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| UBCE[1] | Ubiquitin-conjugating enzyme |
|
XM_003460024 |
|
130 | 59 | SYBR |
| EF1A[1] | EF-1a mRNA for elongation factor 1a |
|
AB075952 |
|
250 | 59 | SYBR |
| GADPH[1] | Glyceraldehyde-3-phosphate dehydrogenase |
|
JN381952 |
|
205 | 59 | SYBR |
| 18S rRNA[1] | 18S ribosomal RNA |
|
JF698683 |
|
111 | 59 | SYBR |
Moleculer Type
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- Ref-Finder method && Related Reference
Contact
- Name: Hua Wen
- Email: wenhua.hb@163.com
- Institution: Key Laboratory of Freshwater Biodiversity Conservation and Utilization of Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China
References
- ↑ 1.0 1.1 1.2 1.3 1.4 Yang C G, Wang X L, Tian J, et al. Evaluation of reference genes for quantitative real-time RT-PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus)[J]. Gene, 2013, 527(1): 183-192.
- ↑ Ijiri S, Kaneko H, Kobayashi T, Wang DS, Sakai F, Paul-Prasanth B, Nakamura M, Nagahama Y. Sexual dimorphic expression of genes in gonads during early differentiation of a teleost fish, the Nile tilapia Oreochromis niloticus. Biol Reprod. 2008 Feb;78(2):333-41. Epub 2007 Oct 17. PubMed PMID: 17942796.
