Oreochromis niloticus
Contents
Description
- Oreochromis niloticus is a species of tilapia, a cichlid fish native to Africa from Egypt south to east and central Africa, and as far west as Gambia. It is also native to Israel, and numerous introduced populations exist outside its natural range. As a gonochoristic teleost fish with an XX/XY sex-determining system, it provides an excellent model for studying gonadal sex differentiation because genetic all-females and all-males are available[1][2].
- Common Name: Nile tilapia
- NCBI Taxonomy
Different Tissues & Bacterial Infection
Internal Control Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| UBCE[1] | Ubiquitin-conjugating enzyme |
|
XM_003460024 |
|
130 | 59 | SYBR |
| EF1A[1] | EF-1a mRNA for elongation factor 1a |
|
AB075952 |
|
250 | 59 | SYBR |
| GADPH[1] | Glyceraldehyde-3-phosphate dehydrogenase |
|
JN381952 |
|
205 | 59 | SYBR |
| 18S rRNA[1] | 18S ribosomal RNA |
|
JF698683 |
|
111 | 59 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- Ref-Finder method && Related Reference
Contact
- Name: Hua Wen
- Email: wenhua.hb@163.com
- Institution: Key Laboratory of Freshwater Biodiversity Conservation and Utilization of Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China
Citation Statistics
Cited by 32 (Based on Google Scholar [2017-06-16])
Vaccination & Infection
Internal Control Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| EF1A[3] | Elongation factor 1 α |
|
AB075952 |
|
250 | 59 | SYBR |
| ACTB[3] | β-actin |
|
XM_003443127 |
|
140 | 59 | SYBR |
Moleculer Type
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- Ref-Finder method && Related Reference
Contact
- Name: Kaiyu Wang
- Email: kywangsicau@126.com
- Institution: Department of Basic Veterinary, Sichuan Agricultural University, Wenjiang District Huimin Road No. 211, Chengdu 611130, China
Citation Statistics
Cited by 32 (Based on Google Scholar [2017-08-10])
References
- ↑ 1.0 1.1 1.2 1.3 1.4 Yang C G, Wang X L, Tian J, et al. Evaluation of reference genes for quantitative real-time RT-PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus)[J]. Gene, 2013, 527(1): 183-192.
- ↑ Ijiri S, Kaneko H, Kobayashi T, Wang DS, Sakai F, Paul-Prasanth B, Nakamura M, Nagahama Y. Sexual dimorphic expression of genes in gonads during early differentiation of a teleost fish, the Nile tilapia Oreochromis niloticus. Biol Reprod. 2008 Feb;78(2):333-41. Epub 2007 Oct 17. PubMed PMID: 17942796.
- ↑ 3.0 3.1 Wang, Erlong, et al. "Evaluation and selection of appropriate reference genes for real-time quantitative PCR analysis of gene expression in Nile tilapia (Oreochromis niloticus) during vaccination and infection." International journal of molecular sciences 16.5 (2015): 9998-10015.
