Difference between revisions of "Paralichthys olivaceus"
Jump to navigation
Jump to search
ICG Expert4 (talk | contribs) |
ICG Expert4 (talk | contribs) |
||
| Line 20: | Line 20: | ||
|align="center"| a -tubulin | |align="center"| a -tubulin | ||
| | | | ||
| − | * | + | *Spleen |
| − | * | + | *Heart |
| − | * | + | *Muscle |
| − | * | + | *Gill |
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/AU050297 '''AU050297'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nucest/AU050297 '''AU050297'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Revision as of 08:14, 19 June 2017
Contents
Description
- Japanese flounder (Paralichthys olivaceus) is an important economic fish species cultured worldwide.Japanese flounder (Paralichthys olivaceus), a species of flatfish, is one of the most important fish species cultured in Asian countries including Japan, Korea, and China.
- Japanese flounder (Paralichthys olivaceus) is an economically important marine fish in Japan, China and Korea. [1] [2]
Before & After Bacterial Infection
Reference Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primer | Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| TUBA [1] | a -tubulin |
|
AU050297 |
|
107 | 60 | SYBR |
Moleculer types
- mRNA
Evaluation Methods
Contact
- Name: Li Sun
- Email: lsun@qdio.ac.cn
- Institute: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China
Viral Infection
Reference Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primer | Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| ACTB[2] | β-actin |
|
EU090804 |
|
135 | 59 | SYBR |
| eIF5A[2] | β-actin |
|
FJ390056 |
|
193 | 59 | SYBR |
| GAPDH[2] | Glyceraldehyde-3-phosphate dehydrogenase |
|
AB029337 |
|
170 | 59 | SYBR |
| UBCE[2] | Ubiquitin-conjugating enzyme |
|
AJ298327 |
|
170 | 59 | SYBR |
| TUBA[2] | α-Tubulin |
|
JN635277 |
|
107 | 59 | SYBR |
| EF1A[2] | Elongation factor-1-α |
|
AB240552 |
|
200 | 59 | SYBR |
Moleculer Types
- mRNA
Evaluation Methods
Contact
- Name: Li Sun
- Email: lsun@qdio.ac.cn
- Institution: Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China
Citation Statistics
Cited by 15 (Based on Google Scholar [2017-06-16])
References
- ↑ 1.0 1.1 Zheng W, Sun L. Evaluation of housekeeping genes as references for quantitative real time RT-PCR analysis of gene expression in Japanese flounder (Paralichthys olivaceus)[J]. Fish & shellfish immunology, 2011, 30(2): 638-645.
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 Zhang J, Hu Y, Sun B, et al. Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection[J]. Veterinary immunology and immunopathology, 2013, 152(3): 303-316.
