Difference between revisions of "Paralichthys olivaceus"

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*Spleen
 
*Spleen

Revision as of 15:05, 22 June 2017

Description

Paralichthys olivaceus-1.jpg
  • Japanese flounder (Paralichthys olivaceus) is an important economic fish species cultured worldwide.Japanese flounder (Paralichthys olivaceus), a species of flatfish, is one of the most important fish species cultured in Asian countries including Japan, Korea, and China.
  • Japanese flounder (Paralichthys olivaceus) is an economically important marine fish in Japan, China and Korea. [1] [2]

Before & After Bacterial Infection

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse] 		Size [bp] Tm [℃] Detection
TUBA [1] α -tubulin
  • Spleen
  • Heart
  • Muscle
  • Gill
 AU050297
  • F:TGACATCACAAACGCCTGCTTC
  • R:GCACCACATCTCCACGGTACAG
 107 60 SYBR

Molecular types

  • mRNA

Evaluation Methods

Contact

  • Name: Li Sun
  • Email: lsun@qdio.ac.cn
  • Institution: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China

Citation Statistics

Cited by 105 (Based on Google Scholar [2017-06-16])

Viral Infection

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse] 		Size [bp] Tm [℃] Detection
ACTB[2] β-actin
  • 24h post-viral infection in brain, gill, live, Heart
  • 72h post-viral infection in brain, gill, liver, Heart
 EU090804
  • F:CGCTGCCTCCTCCTCATC
  • R:ATACCACAAGACTCCATTCCAAG
 135 59 SYBR
eIF5A[2] β-actin
  • 24h post-viral infection in brain, liver
  • 72h post-viral infection in brain, spleen
 FJ390056
  • F:GAACGAAGAAGCCGCCATC
  • R:GTAGGAAGGTTGGAGAACAGAG
 193 59 SYBR
GAPDH[2] Glyceraldehyde-3-phosphate dehydrogenase
  • 24h post-viral infection in gill, muscle
  • 72h post-viral infection in gill, muscle
 AB029337
  • F:CGGCGACACTCACTCCTC
  • R:TCTGATTGGTTGGTTGGTTGG
 170 59 SYBR
UBCE[2] Ubiquitin-conjugating enzyme
  • 24h post-viral infection in heart, muscle, intestine, kidney
  • 72h post-viral infection in in heart, intestine,kidney
 AJ298327
  • F:CACCATCACAGGACCGAATG
  • R:GATGTCAAGGCAGATGCTACC
 170 59 SYBR
TUBA[2] α-Tubulin
  • 24h post-viral infection in heart, muscle, intestine, kidney
  • 72h post-viral infection in heart, intestine, kidney
 JN635277
  • F:TGACATCACAAACGCCTGCTTC
  • R:GCACCACATCTCCACGGTACAG
 107 59 SYBR
EF1A[2] Elongation factor-1-α
  • 24h post-viral infection in kidney, spleen
  • 72h post-viral infection in intestine, spleen, kidney
 AB240552
  • F:CTACAAGTGCGGAGGAATCG
  • R:GTCCAGGAGCGTCAATGATG
 200 59 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Li Sun
  • Email: lsun@qdio.ac.cn
  • Institution: Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China

Citation Statistics

Cited by 15 (Based on Google Scholar [2017-06-16])

References

  1. ↑ 1.0 1.1 Zheng W, Sun L. Evaluation of housekeeping genes as references for quantitative real time RT-PCR analysis of gene expression in Japanese flounder (Paralichthys olivaceus)[J]. Fish & shellfish immunology, 2011, 30(2): 638-645.
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 Zhang J, Hu Y, Sun B, et al. Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection[J]. Veterinary immunology and immunopathology, 2013, 152(3): 303-316.
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