Difference between revisions of "Paralichthys olivaceus"

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=='''Description'''==
 
=='''Description'''==
 +
[[File:Paralichthys olivaceus.png|right|200px|link=Paralichthys olivaceus]]
 +
* '''''Paralichthys olivaceus''''', a species of flatfish, is one of the most economic important fish species cultured in Asian countries including Japan, Korea, and China. It is a temperate marine species and grows fastest at 15 to 25°C. It is proved to be a species with unstable sex-differentiation that is influenced by environmental factors<ref name="ref1"/><ref name="ref2"/>.
 +
* <font color=blue>'''Common Name:'''</font> '''Olive flounder''','''Japanese flounder'''
 +
* [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=8255 <font color=blue>'''NCBI Taxonomy'''</font>]
  
[[File:Paralichthys olivaceus-1.jpg|right|327px|]]
 
* Japanese flounder (Paralichthys olivaceus) is an important economic fish species cultured worldwide.Japanese flounder (Paralichthys olivaceus), a species of flatfish, is one of the most important fish species cultured in Asian countries including Japan, Korea, and China.
 
* Japanese flounder (Paralichthys olivaceus) is an economically important marine fish in Japan, China and Korea.
 
 
=='''''Before & After Bacterial Infection'''''==
 
=='''''Before & After Bacterial Infection'''''==
===Reference Genes===
+
===Internal Control Genes===
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
|-
 
|-
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! style="width=25% font-size:9pt "|Application Scope  
 
! style="width=25% font-size:9pt "|Application Scope  
 
! Accession Number  
 
! Accession Number  
! Primer
+
! Primers (5'-3')<br>[Forward/Reverse]
 
! Size [bp]  
 
! Size [bp]  
 
! Tm [℃]
 
! Tm [℃]
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|-
 
|-
 
|align="center"| TUBA <ref name="ref1"/>
 
|align="center"| TUBA <ref name="ref1"/>
|align="center"| a -tubulin
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|align="center"| α -tubulin
 
|
 
|
*spleen
+
*Spleen
*heart
+
*Heart
*muscle
+
*Muscle
*gill
+
*Gill
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/AU050297 '''AU050297''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/AU050297 '''AU050297''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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|}
 
|}
  
===Moleculer types===
+
===Molecular Types===
 
* mRNA
 
* mRNA
 +
 
===Evaluation Methods===
 
===Evaluation Methods===
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
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*'''Name''':  Li Sun
 
*'''Name''':  Li Sun
 
*'''Email''': lsun@qdio.ac.cn
 
*'''Email''': lsun@qdio.ac.cn
*'''Institute''':  Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China
+
*'''Institution''':  Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China
 +
 
 +
===Citation Statistics===
 +
Cited by [https://scholar.google.com/scholar?cites=16240780444190981113&as_sdt=2005&sciodt=0,5&hl=en '''106'''] (Based on Google Scholar [2017-09-01])
 +
 
 +
=='''''Viral Infection'''''==
 +
 
 +
 
 +
===Internal Control Genes===
 +
{|class="wikitable sortable" style="font-size:10pt; width:100%"
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|-
 +
! Gene Symbol 
 +
! Gene Name
 +
! style="width=25% font-size:9pt "|Application Scope
 +
! Accession Number
 +
! Primers (5'-3')<br>[Forward/Reverse]
 +
! Size [bp]
 +
! Tm [℃]
 +
! Detection
 +
|-
 +
|align="center"| ACTB<ref name="ref2"/>
 +
|align="center"| β-actin
 +
|
 +
* 24h post-viral infection in brain, gill, live, Heart
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* 72h post-viral infection in brain, gill, liver, Heart
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/EU090804 '''EU090804''']
 +
|nowrap style="font-size:9pt"|
 +
* F:CGCTGCCTCCTCCTCATC
 +
* R:ATACCACAAGACTCCATTCCAAG
 +
|align="center"|  135
 +
|align="center"| 59
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| eIF5A<ref name="ref2"/>
 +
|align="center"| β-actin
 +
|
 +
* 24h post-viral infection in brain, liver
 +
* 72h post-viral infection in brain, spleen
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/FJ390056 '''FJ390056''']
 +
|nowrap style="font-size:9pt"|
 +
* F:GAACGAAGAAGCCGCCATC
 +
* R:GTAGGAAGGTTGGAGAACAGAG
 +
|align="center"|  193
 +
|align="center"| 59
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| GAPDH<ref name="ref2"/>
 +
|align="center"| Glyceraldehyde-3-phosphate dehydrogenase
 +
|
 +
* 24h post-viral infection in gill, muscle
 +
* 72h post-viral infection in gill, muscle
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AB029337 '''AB029337''']
 +
|nowrap style="font-size:9pt"|
 +
* F:CGGCGACACTCACTCCTC
 +
* R:TCTGATTGGTTGGTTGGTTGG
 +
|align="center"|  170
 +
|align="center"| 59
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| UBCE<ref name="ref2"/>
 +
|align="center"| Ubiquitin-conjugating enzyme
 +
|
 +
* 24h post-viral infection in heart, muscle, intestine, kidney
 +
* 72h post-viral infection in in heart, intestine,kidney
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AJ298327 '''AJ298327''']
 +
|nowrap style="font-size:9pt"|
 +
* F:CACCATCACAGGACCGAATG
 +
* R:GATGTCAAGGCAGATGCTACC
 +
|align="center"|  170
 +
|align="center"| 59
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| TUBA<ref name="ref2"/>
 +
|align="center"| α-tubulin
 +
|
 +
* 24h post-viral infection in heart, muscle, intestine, kidney
 +
* 72h post-viral infection in heart, intestine, kidney
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/JN635277 '''JN635277''']
 +
|nowrap style="font-size:9pt"|
 +
* F:TGACATCACAAACGCCTGCTTC
 +
* R:GCACCACATCTCCACGGTACAG
 +
|align="center"|  107
 +
|align="center"| 59
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| EF1A<ref name="ref2"/>
 +
|align="center"| Elongation factor-1-α
 +
|
 +
* 24h post-viral infection in kidney, spleen
 +
* 72h post-viral infection in intestine, spleen, kidney
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AB240552 '''AB240552''']
 +
|nowrap style="font-size:9pt"|
 +
* F:CTACAAGTGCGGAGGAATCG
 +
* R:GTCCAGGAGCGTCAATGATG
 +
|align="center"|  200
 +
|align="center"| 59
 +
|align="center"| SYBR
 +
|}
 +
 
 +
===Molecular Types===
 +
*mRNA
 +
 
 +
===Evaluation Methods===
 +
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 +
* [https://moma.dk/normfinder-software '''NormFinder method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/15289330 '''Related Reference''']
 +
 
 +
===Contact===
 +
*'''Name''': Li Sun
 +
*'''Email''': lsun@qdio.ac.cn
 +
*'''Institution''': Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China
 +
===Citation Statistics===
 +
Cited by [https://scholar.google.com/scholar?cites=487178205599622280&as_sdt=2005&sciodt=0,5&hl=en '''17'''] (Based on Google Scholar [2017-09-01])
 +
 
 
=='''References'''==
 
=='''References'''==
 
<references>
 
<references>
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</ref>
 
</ref>
 
<ref name="ref2">
 
<ref name="ref2">
Hu Y, Yoshikawa T, Chung S, Hirono I, Kondo H.Identification of 2 novel type I IFN genes in Japanese flounder, Paralichthys olivaceus.Fish Shellfish Immunol. 2017 May 22;67:7-10. doi: 10.1016/j.fsi.2017.05.054. [Epub ahead of print]PMID: 28546019
+
Zhang J, Hu Y, Sun B, et al. Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection[J]. Veterinary immunology and immunopathology, 2013, 152(3): 303-316.
 
</ref>
 
</ref>
 
</references>
 
</references>
 +
=='''Categories'''==
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[[Category:Animals]]
 +
[[Category:mRNA]]
 +
 +
[[Category:SYBR]]
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[[Category:Tubulin]]
 +
[[Category:Biotic Stress]]  [[Category:Pathological conditions]]  [[Category:Bacterial Infection]]
 +
 +
[[Category:geNorm]]
 +
[[Category:NormFinder]]

Latest revision as of 04:34, 1 September 2017

Description

Paralichthys olivaceus.png
  • Paralichthys olivaceus, a species of flatfish, is one of the most economic important fish species cultured in Asian countries including Japan, Korea, and China. It is a temperate marine species and grows fastest at 15 to 25°C. It is proved to be a species with unstable sex-differentiation that is influenced by environmental factors[1][2].
  • Common Name: Olive flounder,Japanese flounder
  • NCBI Taxonomy

Before & After Bacterial Infection

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
TUBA [1] α -tubulin
  • Spleen
  • Heart
  • Muscle
  • Gill
AU050297
  • F:TGACATCACAAACGCCTGCTTC
  • R:GCACCACATCTCCACGGTACAG
107 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Li Sun
  • Email: lsun@qdio.ac.cn
  • Institution: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China

Citation Statistics

Cited by 106 (Based on Google Scholar [2017-09-01])

Viral Infection

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
ACTB[2] β-actin
  • 24h post-viral infection in brain, gill, live, Heart
  • 72h post-viral infection in brain, gill, liver, Heart
EU090804
  • F:CGCTGCCTCCTCCTCATC
  • R:ATACCACAAGACTCCATTCCAAG
135 59 SYBR
eIF5A[2] β-actin
  • 24h post-viral infection in brain, liver
  • 72h post-viral infection in brain, spleen
FJ390056
  • F:GAACGAAGAAGCCGCCATC
  • R:GTAGGAAGGTTGGAGAACAGAG
193 59 SYBR
GAPDH[2] Glyceraldehyde-3-phosphate dehydrogenase
  • 24h post-viral infection in gill, muscle
  • 72h post-viral infection in gill, muscle
AB029337
  • F:CGGCGACACTCACTCCTC
  • R:TCTGATTGGTTGGTTGGTTGG
170 59 SYBR
UBCE[2] Ubiquitin-conjugating enzyme
  • 24h post-viral infection in heart, muscle, intestine, kidney
  • 72h post-viral infection in in heart, intestine,kidney
AJ298327
  • F:CACCATCACAGGACCGAATG
  • R:GATGTCAAGGCAGATGCTACC
170 59 SYBR
TUBA[2] α-tubulin
  • 24h post-viral infection in heart, muscle, intestine, kidney
  • 72h post-viral infection in heart, intestine, kidney
JN635277
  • F:TGACATCACAAACGCCTGCTTC
  • R:GCACCACATCTCCACGGTACAG
107 59 SYBR
EF1A[2] Elongation factor-1-α
  • 24h post-viral infection in kidney, spleen
  • 72h post-viral infection in intestine, spleen, kidney
AB240552
  • F:CTACAAGTGCGGAGGAATCG
  • R:GTCCAGGAGCGTCAATGATG
200 59 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Li Sun
  • Email: lsun@qdio.ac.cn
  • Institution: Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China

Citation Statistics

Cited by 17 (Based on Google Scholar [2017-09-01])

References

  1. 1.0 1.1 Zheng W, Sun L. Evaluation of housekeeping genes as references for quantitative real time RT-PCR analysis of gene expression in Japanese flounder (Paralichthys olivaceus)[J]. Fish & shellfish immunology, 2011, 30(2): 638-645.
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 Zhang J, Hu Y, Sun B, et al. Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection[J]. Veterinary immunology and immunopathology, 2013, 152(3): 303-316.

Categories