Difference between revisions of "Paralichthys olivaceus"
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*'''Institution''': Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China | *'''Institution''': Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China | ||
===Citation Statistics=== | ===Citation Statistics=== | ||
| − | Cited by '''17''' (Based on Google Scholar [2017- | + | Cited by [https://scholar.google.com/scholar?cites=487178205599622280&as_sdt=2005&sciodt=0,5&hl=en '''17'''] (Based on Google Scholar [2017-09-01]) |
=='''References'''== | =='''References'''== | ||
Latest revision as of 04:34, 1 September 2017
Contents
Description
- Paralichthys olivaceus, a species of flatfish, is one of the most economic important fish species cultured in Asian countries including Japan, Korea, and China. It is a temperate marine species and grows fastest at 15 to 25°C. It is proved to be a species with unstable sex-differentiation that is influenced by environmental factors[1][2].
- Common Name: Olive flounder,Japanese flounder
- NCBI Taxonomy
Before & After Bacterial Infection
Internal Control Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| TUBA [1] | α -tubulin |
|
AU050297 |
|
107 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Li Sun
- Email: lsun@qdio.ac.cn
- Institution: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China
Citation Statistics
Cited by 106 (Based on Google Scholar [2017-09-01])
Viral Infection
Internal Control Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| ACTB[2] | β-actin |
|
EU090804 |
|
135 | 59 | SYBR |
| eIF5A[2] | β-actin |
|
FJ390056 |
|
193 | 59 | SYBR |
| GAPDH[2] | Glyceraldehyde-3-phosphate dehydrogenase |
|
AB029337 |
|
170 | 59 | SYBR |
| UBCE[2] | Ubiquitin-conjugating enzyme |
|
AJ298327 |
|
170 | 59 | SYBR |
| TUBA[2] | α-tubulin |
|
JN635277 |
|
107 | 59 | SYBR |
| EF1A[2] | Elongation factor-1-α |
|
AB240552 |
|
200 | 59 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Li Sun
- Email: lsun@qdio.ac.cn
- Institution: Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China
Citation Statistics
Cited by 17 (Based on Google Scholar [2017-09-01])
References
- ↑ 1.0 1.1 Zheng W, Sun L. Evaluation of housekeeping genes as references for quantitative real time RT-PCR analysis of gene expression in Japanese flounder (Paralichthys olivaceus)[J]. Fish & shellfish immunology, 2011, 30(2): 638-645.
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 Zhang J, Hu Y, Sun B, et al. Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection[J]. Veterinary immunology and immunopathology, 2013, 152(3): 303-316.
