Difference between revisions of "Paralichthys olivaceus"

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*'''Email''': lsun@qdio.ac.cn
 
*'''Email''': lsun@qdio.ac.cn
 
*'''Institute''':  Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China
 
*'''Institute''':  Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China
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 +
=='''''Viral Infection'''''==
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 +
 +
===Reference Genes===
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{|class="wikitable sortable" style="font-size:10pt; width:100%"
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|-
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! Gene Symbol 
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! Gene Name
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! style="width=25%　font-size:9pt　"|Application Scope
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! Accession Number
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! Primer
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! Size [bp]
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! Tm [℃]
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! Detection
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|-
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|align="center"| ACTB<ref name="ref1"/>
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|align="center"| β-actin
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|
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* 24h post-viral infection in brain, gill, live, Heart
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* 72h post-viral infection in brain, gill, liver, Heart
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|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/EU090804 '''EU090804''']
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|nowrap style="font-size:9pt"|
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* F:CGCTGCCTCCTCCTCATC
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* R:ATACCACAAGACTCCATTCCAAG
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|align="center"|  135
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|align="center"| 59
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|align="center"| SYBR
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|-
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|align="center"| eIF5A<ref name="ref1"/>
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|align="center"| β-actin
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|
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* 24h post-viral infection in brain, liver
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* 72h post-viral infection in brain, Spleen
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|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/FJ390056 '''FJ390056''']
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|nowrap style="font-size:9pt"|
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* F:GAACGAAGAAGCCGCCATC
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* R:GTAGGAAGGTTGGAGAACAGAG
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|align="center"|  193
 +
|align="center"| 59
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|align="center"| SYBR
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|-
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|align="center"| GAPDH<ref name="ref1"/>
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|align="center"| Glyceraldehyde-3-phosphate dehydrogenase
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|
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* 24h post-viral infection in Gill, Muscle
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* 72h post-viral infection in Gill, Muscle
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|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AB029337 '''AB029337''']
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|nowrap style="font-size:9pt"|
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* F:CGGCGACACTCACTCCTC
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* R:TCTGATTGGTTGGTTGGTTGG
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|align="center"|  170
 +
|align="center"| 59
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|align="center"| SYBR
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|-
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|align="center"| UBCE<ref name="ref1"/>
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|align="center"| Ubiquitin-conjugating enzyme
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|
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* 24h post-viral infection in Heart, Muscle, Intestine, Kidney
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* 72h post-viral infection in in Heart, Intestine, Kidney
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|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AJ298327 '''AJ298327''']
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|nowrap style="font-size:9pt"|
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* F:CACCATCACAGGACCGAATG
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* R:GATGTCAAGGCAGATGCTACC
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|align="center"|  170
 +
|align="center"| 59
 +
|align="center"| SYBR
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|-
 +
|align="center"| TUBA<ref name="ref1"/>
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|align="center"| α-Tubulin
 +
|
 +
* 24h post-viral infection in Heart, Muscle, Intestine, Kidney
 +
* 72h post-viral infection in Heart, Intestine, Kidney
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/JN635277 '''JN635277''']
 +
|nowrap style="font-size:9pt"|
 +
* F:TGACATCACAAACGCCTGCTTC
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* R:GCACCACATCTCCACGGTACAG
 +
|align="center"|  107
 +
|align="center"| 59
 +
|align="center"| SYBR
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|-
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|align="center"| EF1A<ref name="ref1"/>
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|align="center"| Elongation factor-1-α
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|
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* 24h post-viral infection in Kidney, Spleen
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* 72h post-viral infection in Intestine, Spleen, Kidney
 +
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/AB240552 '''AB240552''']
 +
|nowrap style="font-size:9pt"|
 +
* F:CTACAAGTGCGGAGGAATCG
 +
* R:GTCCAGGAGCGTCAATGATG
 +
|align="center"|  200
 +
|align="center"| 59
 +
|align="center"| SYBR
 +
|}
 +
 
=='''References'''==
 
=='''References'''==
 
<references>
 
<references>

Revision as of 04:49, 17 June 2017

Description

Paralichthys olivaceus-1.jpg
  • Japanese flounder (Paralichthys olivaceus) is an important economic fish species cultured worldwide.Japanese flounder (Paralichthys olivaceus), a species of flatfish, is one of the most important fish species cultured in Asian countries including Japan, Korea, and China.
  • Japanese flounder (Paralichthys olivaceus) is an economically important marine fish in Japan, China and Korea.

Before & After Bacterial Infection

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
TUBA [1] a -tubulin
  • spleen
  • heart
  • muscle
  • gill
 AU050297
  • F:TGACATCACAAACGCCTGCTTC
  • R:GCACCACATCTCCACGGTACAG
 107 60 SYBR

Moleculer types

  • mRNA

Evaluation Methods

Contact

  • Name: Li Sun
  • Email: lsun@qdio.ac.cn
  • Institute: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China

Viral Infection

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
ACTB[1] β-actin
  • 24h post-viral infection in brain, gill, live, Heart
  • 72h post-viral infection in brain, gill, liver, Heart
 EU090804
  • F:CGCTGCCTCCTCCTCATC
  • R:ATACCACAAGACTCCATTCCAAG
 135 59 SYBR
eIF5A[1] β-actin
  • 24h post-viral infection in brain, liver
  • 72h post-viral infection in brain, Spleen
 FJ390056
  • F:GAACGAAGAAGCCGCCATC
  • R:GTAGGAAGGTTGGAGAACAGAG
 193 59 SYBR
GAPDH[1] Glyceraldehyde-3-phosphate dehydrogenase
  • 24h post-viral infection in Gill, Muscle
  • 72h post-viral infection in Gill, Muscle
 AB029337
  • F:CGGCGACACTCACTCCTC
  • R:TCTGATTGGTTGGTTGGTTGG
 170 59 SYBR
UBCE[1] Ubiquitin-conjugating enzyme
  • 24h post-viral infection in Heart, Muscle, Intestine, Kidney
  • 72h post-viral infection in in Heart, Intestine, Kidney
 AJ298327
  • F:CACCATCACAGGACCGAATG
  • R:GATGTCAAGGCAGATGCTACC
 170 59 SYBR
TUBA[1] α-Tubulin
  • 24h post-viral infection in Heart, Muscle, Intestine, Kidney
  • 72h post-viral infection in Heart, Intestine, Kidney
 JN635277
  • F:TGACATCACAAACGCCTGCTTC
  • R:GCACCACATCTCCACGGTACAG
 107 59 SYBR
EF1A[1] Elongation factor-1-α
  • 24h post-viral infection in Kidney, Spleen
  • 72h post-viral infection in Intestine, Spleen, Kidney
 AB240552
  • F:CTACAAGTGCGGAGGAATCG
  • R:GTCCAGGAGCGTCAATGATG
 200 59 SYBR

References

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Zheng W, Sun L. Evaluation of housekeeping genes as references for quantitative real time RT-PCR analysis of gene expression in Japanese flounder (Paralichthys olivaceus)[J]. Fish & shellfish immunology, 2011, 30(2): 638-645.

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