Description
- Japanese flounder (Paralichthys olivaceus) is an important economic fish species cultured worldwide.Japanese flounder (Paralichthys olivaceus), a species of flatfish, is one of the most important fish species cultured in Asian countries including Japan, Korea, and China.
- Japanese flounder (Paralichthys olivaceus) is an economically important marine fish in Japan, China and Korea. [1] [2]
Before & After Bacterial Infection
Reference Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| TUBA [1]
|
a -tubulin
|
|
AU050297
|
- F:TGACATCACAAACGCCTGCTTC
- R:GCACCACATCTCCACGGTACAG
|
107
|
60
|
SYBR
|
Moleculer types
Evaluation Methods
Contact
- Name: Li Sun
- Email: lsun@qdio.ac.cn
- Institute: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China
Viral Infection
Reference Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| ACTB[2]
|
β-actin
|
- 24h post-viral infection in brain, gill, live, Heart
- 72h post-viral infection in brain, gill, liver, Heart
|
EU090804
|
- F:CGCTGCCTCCTCCTCATC
- R:ATACCACAAGACTCCATTCCAAG
|
135
|
59
|
SYBR
|
| eIF5A[2]
|
β-actin
|
- 24h post-viral infection in brain, liver
- 72h post-viral infection in brain, Spleen
|
FJ390056
|
- F:GAACGAAGAAGCCGCCATC
- R:GTAGGAAGGTTGGAGAACAGAG
|
193
|
59
|
SYBR
|
| GAPDH[2]
|
Glyceraldehyde-3-phosphate dehydrogenase
|
- 24h post-viral infection in Gill, Muscle
- 72h post-viral infection in Gill, Muscle
|
AB029337
|
- F:CGGCGACACTCACTCCTC
- R:TCTGATTGGTTGGTTGGTTGG
|
170
|
59
|
SYBR
|
| UBCE[2]
|
Ubiquitin-conjugating enzyme
|
- 24h post-viral infection in Heart, Muscle, Intestine, Kidney
- 72h post-viral infection in in Heart, Intestine, Kidney
|
AJ298327
|
- F:CACCATCACAGGACCGAATG
- R:GATGTCAAGGCAGATGCTACC
|
170
|
59
|
SYBR
|
| TUBA[2]
|
α-Tubulin
|
- 24h post-viral infection in Heart, Muscle, Intestine, Kidney
- 72h post-viral infection in Heart, Intestine, Kidney
|
JN635277
|
- F:TGACATCACAAACGCCTGCTTC
- R:GCACCACATCTCCACGGTACAG
|
107
|
59
|
SYBR
|
| EF1A[2]
|
Elongation factor-1-α
|
- 24h post-viral infection in Kidney, Spleen
- 72h post-viral infection in Intestine, Spleen, Kidney
|
AB240552
|
- F:CTACAAGTGCGGAGGAATCG
- R:GTCCAGGAGCGTCAATGATG
|
200
|
59
|
SYBR
|
Moleculer Types
Evaluation Methods
Contact
- Name: Li Sun
- Email: lsun@qdio.ac.cn
- Institution: Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China
Citation Statistics
Cited by 15 (Based on Google Scholar [2017-06-16])
References
- ↑ 1.0 1.1
Zheng W, Sun L. Evaluation of housekeeping genes as references for quantitative real time RT-PCR analysis of gene expression in Japanese flounder (Paralichthys olivaceus)[J]. Fish & shellfish immunology, 2011, 30(2): 638-645.
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6
Zhang J, Hu Y, Sun B, et al. Selection of normalization factors for quantitative real time RT-PCR studies in Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus) under conditions of viral infection[J]. Veterinary immunology and immunopathology, 2013, 152(3): 303-316.
