Phaseolus vulgaris
Contents
Description
- Common bean is the most important legume for direct human consumption, providing an important source of minerals and proteins. Worldwide,common bean is the most consumed legume, providing up to 15% of total daily calories and 36% of total daily protein in parts of Africa and the Americas (see URLs). More than 200 million people in subSaharan Africa depend on the common bean as a primary staple. It has many health-beneficial, nutrients whose concentrations are heritable, and increasing the concentrations of these nutrients is abreeding objective worldwide.Multiple lines of evidence have shown that wild common bean is organized in two geographically isolated and genetically differentiated wild gene pools (Mesoamerican and Andean) that diverged from a common ancestral wild population more than 100,000 years ago. From these wild gene pools, nearly 8,000 years ago, common bean was independently domesticated in what is now Mexico and in South America, and these domestication events were followed by local adaptations resulting in landraces with distinct characteristics[1] [2].
- Common Name: String bean, Bean, Common Bean
- NCBI Taxonomy
Biotic & Abiotic Stresses
Reference Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
IDE[1] | Insulin degrading enzyme |
|
171614244 |
|
156 | 81.1 | SYBR |
Act11[1] | Actin-11 |
|
62703083 |
|
190 | 79.4 | SYBR |
Skip16[1] | SKP1/ASK-interacting protein 16 |
|
187434529 |
|
163 | 81.5 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
- Ref-Finder method && Related Reference
- ΔCt approach method && Related Reference
Contact
- Name: Danielle Gregorio Gomes Caldas
- Email: dcaldas@cena.usp.br
- Institution: Center of Nuclear Energy for Agriculture, Laboratory of Cellular and Molecular Biology, University of São Paulo, P. O. Box 96 CEP, Piracicaba, SP 13400-970, Brazil
Citation Statistics
Cited by 17 (Based on Google Scholar [2017-06-01])
References
- ↑ 1.0 1.1 1.2 1.3 Koči J, Šimo L, Park Y. Validation of internal reference genes for real-time quantitative polymerase chain reaction studies in the tick, Ixodes scapularis (Acari: Ixodidae)[J]. Journal of medical entomology, 2013, 50(1): 79-84.
- ↑ Schmutz J, McClean P E, Mamidi S, et al. A reference genome for common bean and genome-wide analysis of dual domestications[J]. Nature genetics, 2014, 46(7): 707-713.