Difference between revisions of "Pleurotus ostreatus"

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=='''Description'''==
 
=='''Description'''==
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[[File:Pleurotus_ostreatus-1|right|230px]]
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[[File:Pleurotus ostreatus-1.jpg|right|200px|link=Pleurotus ostreatus]]
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*'''''Pleurotus ostreatus''''' is an efficient producer of extracellular enzymes, such as laccases (Lacs; EC 1.10.3.2) and he basidiomycete Pleurotus ostreatus is an efficient producer of manganese peroxidases (MnPs; EC 1.11.1.13), which are notable for their application in multiple industrial and biotechnological processes. It has a special relevance in agroindustry due to its worldwide cultivation for mushroom production, and it has also been studied for its nutritional and medicinal value.<ref name="ref1"/>
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*'''''Pleurotus ostreatus''''' is an efficient producer of extracellular enzymes, such as laccases (Lacs; EC 1.10.3.2) and he basidiomycete Pleurotus ostreatus is an efficient producer of manganese peroxidases (MnPs; EC 1.11.1.13), which are notable for their application in multiple industrial and biotechnological processes. It has a special relevance in agroindustry due to its worldwide cultivation for mushroom production, and it has also been studied for its nutritional and medicinal value <ref name="ref1"/>.
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* <font color=blue>'''Common Name:'''</font> '''oyster mushroom'''
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* <font color=blue>'''Common Name:'''</font> '''Oyster mushroom'''
 
*[https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=5322&lvl=3&lin=f&keep=1&srchmode=1&unlock<font color=blue>'''NCBI Taxonomy'''</font>]
 
*[https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=5322&lvl=3&lin=f&keep=1&srchmode=1&unlock<font color=blue>'''NCBI Taxonomy'''</font>]
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=='''''Various Culture Conditions'''''==
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===Internal Control Genes===
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{|class="wikitable sortable" style="font-size:10pt; width:100%"
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|-
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! Gene Symbol 
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! Gene Name
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! style="width=25%　font-size:9pt　"|Application Scope
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! Accession Number
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! Primers (5'-3')<br>[Forward/Reverse]
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! Size [bp]
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! Tm [℃]
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! Detection
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|-
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|align="center"| sar1<ref name="ref1"/>
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|align="center"| Secretion-associated Ras-related 1
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|
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*SmF cultures
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|align="center"| NA
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|nowrap style="font-size:9pt"|
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* F:GGATAGTCTTCCTCGTCGATAG
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* R:GGGTGCGTCAATCTTGTTAC
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|align="center"| 133
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|align="center"| 63
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|align="center"| SYBR
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|-
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|align="center"| pep<ref name="ref1"/>
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|align="center"|  strain EPGS 
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|
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*SmF cultures
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|align="center"| NA
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|nowrap style="font-size:9pt"|
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* F:TGATTCCAGAGGACAAGGACGCAA
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* R:AAATCTTCCGCGATACGGGTCACT
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|align="center"| 148
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|align="center"| 63
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|align="center"| SYBR
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|-
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|align="center"| phos<ref name="ref1"/>
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|align="center"| phosphate-binding protein PhoS
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|
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*SmF cultures
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|align="center"| NA
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|nowrap style="font-size:9pt"|
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* F:CATCGCAAATCATCGATCGCACCA
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* R:GCTCTCCAGCCAATGCACCAATTT
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|align="center"| 125
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|align="center"| 63
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|align="center"| SYBR
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|}
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===Molecular Types===
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* mRNA
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===Evaluation Methods===
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* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
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* [https://moma.dk/normfinder-software '''NormFinder method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/15289330 '''Related Reference''']
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* [http://www.gene-quantification.com/bestkeeper.html '''BestKeeper method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/15127793 '''Related Reference''']
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===Contact===
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*'''Name''': Lucía Ramírez
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*'''Email''': lramirez@unavarra.es
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*'''Institution''': Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, Pamplona, Navarre, Spain
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===Citation Statistics===
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Cited by [https://scholar.google.com/scholar?cites=2826155834730814237&as_sdt=2005&sciodt=0,5&hl=en '''7'''] (Based on Google Scholar [2017-09-01])
  
 
=='''References'''==
 
=='''References'''==
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</ref>
 
</ref>
 
</references>
 
</references>
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=='''Categories'''==
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[[Category:Various Culture Conditions]]
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[[Category:sar1]] [[Category:pep]] [[Category:phos]]
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[[Category:Fungi]]
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[[Category:SYBR]]
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[[Category:geNorm]]
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[[Category:NormFinder]]
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[[Category:BestKeeper]]

Latest revision as of 05:22, 1 September 2017

Description

Pleurotus ostreatus-1.jpg
  • Pleurotus ostreatus is an efficient producer of extracellular enzymes, such as laccases (Lacs; EC 1.10.3.2) and he basidiomycete Pleurotus ostreatus is an efficient producer of manganese peroxidases (MnPs; EC 1.11.1.13), which are notable for their application in multiple industrial and biotechnological processes. It has a special relevance in agroindustry due to its worldwide cultivation for mushroom production, and it has also been studied for its nutritional and medicinal value [1].
  • Common Name: Oyster mushroom
  • NCBI Taxonomy

Various Culture Conditions

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse] 		Size [bp] Tm [℃] Detection
sar1[1] Secretion-associated Ras-related 1
  • SmF cultures
 NA
  • F:GGATAGTCTTCCTCGTCGATAG
  • R:GGGTGCGTCAATCTTGTTAC
 133 63 SYBR
pep[1]  strain EPGS 
  • SmF cultures
 NA
  • F:TGATTCCAGAGGACAAGGACGCAA
  • R:AAATCTTCCGCGATACGGGTCACT
 148 63 SYBR
phos[1] phosphate-binding protein PhoS
  • SmF cultures
 NA
  • F:CATCGCAAATCATCGATCGCACCA
  • R:GCTCTCCAGCCAATGCACCAATTT
 125 63 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Lucía Ramírez
  • Email: lramirez@unavarra.es
  • Institution: Genetics and Microbiology Research Group, Department of Agrarian Production, Public University of Navarre, Pamplona, Navarre, Spain

Citation Statistics

Cited by 7 (Based on Google Scholar [2017-09-01])

References

  1. ↑ 1.0 1.1 1.2 1.3 Castanera R, López-Varas L, Pisabarro AG, Ramírez L. Validation of Reference Genes for Transcriptional Analyses in Pleurotus ostreatus by Using Reverse Transcription-Quantitative PCR. Appl Environ Microbiol. 2015 Jun 15;81(12):4120-9. doi: 10.1128/AEM.00402-15. Epub 2015 Apr 10. PubMed PMID: 25862220; PubMed Central PMCID: PMC4524153.

Categories

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