Difference between revisions of "Raphanus sativus"

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=='''Description'''==
 
=='''Description'''==
=='''''Tissue types & Cultivars & Developmental Stages'''''==
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[[File:Raphanus sativus.png|right|200px|link=Raphanus sativus]]
===Reference Genes===
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* '''''Raphanus sativus L.''''', an annual or biennial herb of the Brassicaceae family, is an economically important root vegetable crop produced throughout the world. It is a typical long-day plant and requires vernalization to promote bolting and flowering. The edible part of radish is its taproot, which is an excellent source of carbohydrates, dietary fiber, and essential mineral and organic nutrients to human beings. Radish roots also contain valuable phytochemicals and have been used for many medicinal purposes<ref name="ref1"/><ref name="ref2"/>.
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* <font color=blue>'''Common Name:'''</font> '''Radish'''
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* [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=3726 <font color=blue>'''NCBI Taxonomy'''</font>]
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=='''''Different Tissues & Cultivars & Developmental Stages'''''==
 +
===Internal Control Genes===
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
|-
 
|-
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! style="width=25% font-size:9pt "|Application Scope  
 
! style="width=25% font-size:9pt "|Application Scope  
 
! Accession Number  
 
! Accession Number  
! Primer
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! Primers (5'-3')<br>[Forward/Reverse]
 
! Size [bp]  
 
! Size [bp]  
 
! Tm [℃]
 
! Tm [℃]
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|align="center"| Translation elongation factor 2  
 
|align="center"| Translation elongation factor 2  
 
|
 
|
*tissue types;*cultivars;*photoperiodic;*vernalization treatments;* developmental stages
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*Tissue types
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*Cultivars
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*Photoperiodic
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*Vernalization treatments
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*Developmental stages
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/FY430003 '''FY430003''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/FY430003 '''FY430003''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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|align="center"| RNA polymerase-II transcription factor
 
|align="center"| RNA polymerase-II transcription factor
 
|
 
|
*tissue types;*cultivars;*photoperiodic;*vernalization treatments;* developmental stages
+
*Tissue types
 +
*Cultivars
 +
*Photoperiodic
 +
*Vernalization treatments
 +
*Developmental stages
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/FY438464 '''FY438464''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/FY438464 '''FY438464''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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|align="center"| Actin 2/7
 
|align="center"| Actin 2/7
 
|
 
|
*tissue types;*cultivars;*photoperiodic;*vernalization treatments;* developmental stages
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*Tissue types
 +
*Cultivars
 +
*Photoperiodic
 +
*Vernalization treatments
 +
*Developmental stages
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/FY430005 '''FY430005''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/FY430005 '''FY430005''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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|align="center"| SYBR
 
|align="center"| SYBR
 
|}
 
|}
===Moleculer types===
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 +
===Molecular Types===
 
* mRNA
 
* mRNA
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===Evaluation Methods===
 
===Evaluation Methods===
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
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*'''Name''': Liwang Liu
 
*'''Name''': Liwang Liu
 
*'''Email''': nauliulw@njau.edu.cn
 
*'''Email''': nauliulw@njau.edu.cn
*'''Institute''': National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture of PR China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China
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*'''Institution''': National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture of PR China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China
  
===Contact===
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===Citation Statistics===
*'''Name''':  Liwang Liu
+
Cited by [https://scholar.google.com/scholar?cites=3128533758459439052&as_sdt=2005&sciodt=0,5&hl=en'''54'''] (Based on Google Scholar [2017-09-01])
*'''Email''': nauliulw@njau.edu.cn
 
*'''Institution''': National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture of PR China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China
 
  
 
=='''References'''==
 
=='''References'''==
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<ref name="ref1">
 
<ref name="ref1">
 
Xu Y, Zhu X, Gong Y, et al. Evaluation of reference genes for gene expression studies in radish (Raphanus sativus L.) using quantitative real-time PCR[J]. Biochemical and biophysical research communications, 2012, 424(3): 398-403.
 
Xu Y, Zhu X, Gong Y, et al. Evaluation of reference genes for gene expression studies in radish (Raphanus sativus L.) using quantitative real-time PCR[J]. Biochemical and biophysical research communications, 2012, 424(3): 398-403.
 +
</ref>
 +
<ref name="ref2">
 +
Wang Y, Pan Y, Liu Z, et al. De novo transcriptome sequencing of radish (Raphanus sativus L.) and analysis of major genes involved in glucosinolate metabolism. BMC Genomics. 2013;14(1):836. doi:10.1186/1471-2164-14-836. PMCID: PMC4046679.
 
</ref>
 
</ref>
 
</references>
 
</references>
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 +
=='''Categories'''==
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[[Category:Plants]][[Category:mRNA]][[Category:SYBR]][[Category:ACT]][[Category:EF1α]][[Category:polA]][[Category:Different Genotypes]][[Category:Different Tissues]][[Category:Different Developmental Stages]][[Category:geNorm]][[Category:NormFinder]]

Latest revision as of 07:25, 1 September 2017

Description

Raphanus sativus.png
  • Raphanus sativus L., an annual or biennial herb of the Brassicaceae family, is an economically important root vegetable crop produced throughout the world. It is a typical long-day plant and requires vernalization to promote bolting and flowering. The edible part of radish is its taproot, which is an excellent source of carbohydrates, dietary fiber, and essential mineral and organic nutrients to human beings. Radish roots also contain valuable phytochemicals and have been used for many medicinal purposes[1][2].
  • Common Name: Radish
  • NCBI Taxonomy

Different Tissues & Cultivars & Developmental Stages

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
TEF2[1] Translation elongation factor 2
  • Tissue types
  • Cultivars
  • Photoperiodic
  • Vernalization treatments
  • Developmental stages
FY430003
  • F:AAGAAGATTTGGGCGTTTGG
  • R:CCAGCAACAACAGAATCCTT
107 58 SYBR
RPII[1] RNA polymerase-II transcription factor
  • Tissue types
  • Cultivars
  • Photoperiodic
  • Vernalization treatments
  • Developmental stages
FY438464
  • F:ATCACGCTAAATGGTCTCCT
  • R:GCTGCTCTCAATCAAGTCAATC
122 58 SYBR
ACT[1] Actin 2/7
  • Tissue types
  • Cultivars
  • Photoperiodic
  • Vernalization treatments
  • Developmental stages
FY430005
  • F:GCATCACACTTTCTACAAC
  • R:CCTGGATAGCAACATACAT
155 58 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Liwang Liu
  • Email: nauliulw@njau.edu.cn
  • Institution: National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture of PR China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China

Citation Statistics

Cited by 54 (Based on Google Scholar [2017-09-01])

References

  1. 1.0 1.1 1.2 1.3 Xu Y, Zhu X, Gong Y, et al. Evaluation of reference genes for gene expression studies in radish (Raphanus sativus L.) using quantitative real-time PCR[J]. Biochemical and biophysical research communications, 2012, 424(3): 398-403.
  2. Wang Y, Pan Y, Liu Z, et al. De novo transcriptome sequencing of radish (Raphanus sativus L.) and analysis of major genes involved in glucosinolate metabolism. BMC Genomics. 2013;14(1):836. doi:10.1186/1471-2164-14-836. PMCID: PMC4046679.

Categories