Description
Tissue types & Cultivars & Developmental Stages
Reference Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| TEF2[1]
|
Translation elongation factor 2
|
- tissue types；*cultivars；*photoperiodic；*vernalization treatments；* developmental stages
|
FY430003
|
- F:AAGAAGATTTGGGCGTTTGG
- R:CCAGCAACAACAGAATCCTT
|
107
|
58
|
SYBR
|
| RPII[1]
|
RNA polymerase-II transcription factor
|
- tissue types；*cultivars；*photoperiodic；*vernalization treatments；* developmental stages
|
FY438464
|
- F:ATCACGCTAAATGGTCTCCT
- R:GCTGCTCTCAATCAAGTCAATC
|
122
|
58
|
SYBR
|
| ACT[1]
|
Actin 2/7
|
- tissue types；*cultivars；*photoperiodic；*vernalization treatments；* developmental stages
|
FY430005
|
- F:GCATCACACTTTCTACAAC
- R:CCTGGATAGCAACATACAT
|
155
|
58
|
SYBR
|
Moleculer types
Evaluation Methods
Contact
- Name: Liwang Liu
- Email: nauliulw@njau.edu.cn
- Institute: National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture of PR China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China
Reference Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| TEF2[1]
|
Translation elongation factor 2
|
|
FY430003
|
- F:AAGAAGATTTGGGCGTTTGG
- R:CCAGCAACAACAGAATCCTT
|
107
|
60
|
SYBR
|
| RPII[1]
|
RNA polymerase-II transcription factor
|
|
FY438464
|
- F:ATCACGCTAAATGGTCTCCT
- R:GCTGCTCTCAATCAAGTCAATC
|
122
|
60
|
SYBR
|
| ACT[1]
|
Actin2/7
|
|
FY430005
|
- F:GCATCACACTTTCTACAAC
- R:CCTGGATAGCAACATACAT
|
155
|
60
|
SYBR
|
Moleculer Types
Adopted Evaluation Algorithms
Contact
- Name: Liwang Liu
- Email: nauliulw@njau.edu.cn
- Institution: National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture of PR China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5
Xu Y, Zhu X, Gong Y, et al. Evaluation of reference genes for gene expression studies in radish (Raphanus sativus L.) using quantitative real-time PCR[J]. Biochemical and biophysical research communications, 2012, 424(3): 398-403.
References
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