Raphanus sativus

Tissue types & Cultivars　& Developmental Stages

Reference Genes

Gene Symbol	Gene Name	Application Scope	Accession Number	Primer	Size [bp]	Tm [℃]	Detection
TEF2	Translation elongation factor 2	tissue types；*cultivars；*photoperiodic；*vernalization treatments；* developmental stages	FY430003	F:AAGAAGATTTGGGCGTTTGG R:CCAGCAACAACAGAATCCTT	107	58	SYBR
RPII	RNA polymerase-II transcription factor	tissue types；*cultivars；*photoperiodic；*vernalization treatments；* developmental stages	FY438464	F:ATCACGCTAAATGGTCTCCT R:GCTGCTCTCAATCAAGTCAATC	122	58	SYBR
ACT	Actin 2/7	tissue types；*cultivars；*photoperiodic；*vernalization treatments；* developmental stages	FY430005	F:GCATCACACTTTCTACAAC R:CCTGGATAGCAACATACAT	155	58	SYBR

Moleculer types

• mRNA

Evaluation Methods

• geNorm method && Related Reference
• NormFinder method && Related Reference

Contact

• Name: Liwang Liu
• Email: nauliulw@njau.edu.cn
• Institute: National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture of PR China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China

Adopted Evaluation Algorithms

• geNorm method && Related Reference
• NormFinder method && Related Reference

Contact

• Name: Liwang Liu
• Email: nauliulw@njau.edu.cn
• Institution: National Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture of PR China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China