Difference between revisions of "Rattus norvegicus"
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− | ==''' | + | =='''Description'''== |
+ | [[File:Rattus norvegicus.png|right|200px|link=Rattus norvegicus]] | ||
+ | * '''''Rattus norvegicus''''' is one of the best known and most common rats. Thought to have originated in northern China, this rodent has now spread to all continents except Antarctica, and is the dominant rat in Europe and much of North America—making it by at least this particular definition the most successful mammal on the planet alongside humans [https://en.wikipedia.org/wiki/Brown_rat (Modified from Wikipedia)]. | ||
+ | * <font color=blue>'''Common Name:'''</font> '''Rat''', '''Brown rat''' | ||
+ | * [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10116 <font color=blue>'''NCBI Taxonomy'''</font>] | ||
− | === | + | =='''''Eerebral Ischaemia Model'''''== |
+ | |||
+ | ===Internal Control Genes=== | ||
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width:35% font-size:9pt "|Application Scope | ! style="width:35% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 16: | Line 22: | ||
|align="center"| Ribosomal Protein L13A | |align="center"| Ribosomal Protein L13A | ||
|style="width:55% font-size:9pt "| | |style="width:55% font-size:9pt "| | ||
− | * | + | * In-vitro model of cerebral ischaemia |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_173340 '''NM_173340'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_173340 '''NM_173340'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 28: | Line 34: | ||
|align="center"| Succinate dehydrogenase complex, subunit A | |align="center"| Succinate dehydrogenase complex, subunit A | ||
| | | | ||
− | * | + | * In-vivo and in-vitro models of cerebral ischaemia |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_130428.1 '''NM_130428.1'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_130428.1 '''NM_130428.1'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 40: | Line 46: | ||
|align="center"| Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide | |align="center"| Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide | ||
| | | | ||
− | * | + | * In-vivo model of cerebral ischaemia |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013011.3 '''NM_013011.3'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013011.3 '''NM_013011.3'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 50: | Line 56: | ||
|} | |} | ||
− | === | + | ===Molecular Types=== |
* mRNA | * mRNA | ||
Line 60: | Line 66: | ||
*'''Name''': Judith Mallolas | *'''Name''': Judith Mallolas | ||
*'''Email''': jmallolas.girona.ics@gencat.cat | *'''Email''': jmallolas.girona.ics@gencat.cat | ||
− | *''' | + | *'''Institution''': Servei de Neurologia, Fundació Privada Institut d'Investigació Biomèdica de Girona Dr. Josep Trueta (IdIBGi), Hospital Universitari de Girona Dr. Josep Trueta, Girona, Spain |
+ | |||
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=5013758709212631597&as_sdt=2005&sciodt=0,5&hl=en '''82'''] (Based on Google Scholar [2017-09-01]) | ||
− | ==''' | + | =='''''Asphyxial Cardiac Arrest Model'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 76: | Line 85: | ||
|- | |- | ||
|align="center"| CypA<ref name="ref2"/> | |align="center"| CypA<ref name="ref2"/> | ||
− | |align="center"| | + | |align="center"| Cyclophilin A |
| | | | ||
* 4 and 21 days post injury | * 4 and 21 days post injury | ||
* 7 days post treatment | * 7 days post treatment | ||
+ | * Treated with the anti-inflammatory and anti-apoptotic drug minocycline | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/M19533 '''M19533 '''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/M19533 '''M19533 '''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 89: | Line 99: | ||
|- | |- | ||
|align="center"| Pgk1<ref name="ref2"/> | |align="center"| Pgk1<ref name="ref2"/> | ||
− | |align="center"| | + | |align="center"| Phosphoglycerate kinase 1 |
| | | | ||
* 4 and 21 days post injury | * 4 and 21 days post injury | ||
+ | * Treated with the anti-inflammatory and anti-apoptotic drug minocycline | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_053291 '''NM_053291'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_053291 '''NM_053291'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 101: | Line 112: | ||
|- | |- | ||
|align="center"| Gapdh<ref name="ref2"/> | |align="center"| Gapdh<ref name="ref2"/> | ||
− | |align="center"| | + | |align="center"| Glyceraldehyde-3-phosphate dehydrogenase |
| | | | ||
* 7 days post injury | * 7 days post injury | ||
+ | * Treated with the anti-inflammatory and anti-apoptotic drug minocycline | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_017008 '''NM_017008'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_017008 '''NM_017008'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 112: | Line 124: | ||
|align="center"| SYBR | |align="center"| SYBR | ||
|} | |} | ||
− | === | + | |
+ | ===Molecular Types=== | ||
* mRNA | * mRNA | ||
Line 122: | Line 135: | ||
*'''Name''': Kristina Langnaese | *'''Name''': Kristina Langnaese | ||
*'''Email''': kristina.langnaese@med.ovgu.de | *'''Email''': kristina.langnaese@med.ovgu.de | ||
− | *''' | + | *'''Institution''': nstitute of Medical Neurobiology, University of Magdeburg, Leipziger Str. 44, D-39120 Magdeburg, Germany |
− | =='''Cardiosphere-derived Cells'''== | + | ===Citation Statistics=== |
− | === | + | Cited by [https://scholar.google.com/scholar?cites=9456573743372315036&as_sdt=2005&sciodt=0,5&hl=en '''84'''] (Based on Google Scholar [2017-09-01]) |
+ | |||
+ | =='''''Cardiosphere-derived Cells'''''== | ||
+ | ===Internal Control Genes=== | ||
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 138: | Line 154: | ||
|- | |- | ||
|align="center"| GAPDH<ref name="ref3"/> | |align="center"| GAPDH<ref name="ref3"/> | ||
− | |align="center"| | + | |align="center"| Glyceraldehyde-3-phosphate dehydrogenase |
| | | | ||
− | * | + | * Neonatal and adult rat CDCs preconditioned by hypoxia or PHDIs |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_017008 '''NM_017008'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_017008 '''NM_017008'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
* F:GGGTGTGAACCACGAGAAAT | * F:GGGTGTGAACCACGAGAAAT | ||
− | * R: | + | * R:ACTGTGGTCATGAGCCCTTC |
|align="center"| NA | |align="center"| NA | ||
|align="center"| 60 | |align="center"| 60 | ||
|align="center"| SYBR | |align="center"| SYBR | ||
|- | |- | ||
− | |align="center"| | + | |align="center"| ACTB<ref name="ref3"/> |
− | |align="center"| | + | |align="center"| β-actin |
| | | | ||
− | * | + | * Neonatal and adult rat CDCs preconditioned by hypoxia or PHDIs |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_031144 '''NM_031144'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_031144 '''NM_031144'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 161: | Line 177: | ||
|align="center"| SYBR | |align="center"| SYBR | ||
|} | |} | ||
− | === | + | |
+ | ===Molecular Types=== | ||
* mRNA | * mRNA | ||
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*'''Name''': Carolyn A. Carr | *'''Name''': Carolyn A. Carr | ||
*'''Email''': carolyn.carr@dpag.ox.ac.uk | *'''Email''': carolyn.carr@dpag.ox.ac.uk | ||
− | *''' | + | *'''Institution''': Cardiac Metabolism Research Group, Department of Physiology, Anatomy & Genetics, University of Oxford, Sherrington Building, Parks Road, Oxford OX1 3PT, UK |
− | ==''' | + | ===Citation Statistics=== |
− | === | + | Cited by [https://scholar.google.com/scholar?cites=15278623732676908853&as_sdt=2005&sciodt=0,5&hl=en '''54'''] (Based on Google Scholar [2017-08-10]) |
+ | |||
+ | =='''''Adipose Tissue'''''== | ||
+ | ===Internal Control Genes=== | ||
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
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|align="center"| Glyceraldehyde-3-phosphate dehydrogenase | |align="center"| Glyceraldehyde-3-phosphate dehydrogenase | ||
| | | | ||
− | * eWAT | + | * eWAT types of rat adipose tissue |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_017008.4 '''NM_017008.4'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_017008.4 '''NM_017008.4'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 198: | Line 218: | ||
|align="center"| SYBR | |align="center"| SYBR | ||
|- | |- | ||
− | |align="center"| | + | |align="center"| ACTB<ref name="ref4"/> |
|align="center"| β-actin | |align="center"| β-actin | ||
| | | | ||
− | * iBeAT & BAT | + | * iBeAT & BAT types of rat adipose tissue |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_031144.3 '''NM_031144.3'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_031144.3 '''NM_031144.3'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 210: | Line 230: | ||
|align="center"| SYBR | |align="center"| SYBR | ||
|} | |} | ||
− | === | + | |
+ | ===Molecular Types=== | ||
* mRNA | * mRNA | ||
Line 221: | Line 242: | ||
*'''Name''': You-E Yan | *'''Name''': You-E Yan | ||
*'''Email''': yanyoue@whu.edu.cn | *'''Email''': yanyoue@whu.edu.cn | ||
− | *''' | + | *'''Institution''': Department of Pharmacology, Basic Medical School of Wuhan University, 185, DongHu Road, Wuhan 430071, China |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=5705193182835038858&as_sdt=2005&sciodt=0,5&hl=en '''8'''] (Based on Google Scholar [2017-09-01]) | ||
− | =='''Intestinal Epithelial Cells'''== | + | =='''''Intestinal Epithelial Cells'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 239: | Line 262: | ||
|align="center"| Peptidylprolyl isomerase B | |align="center"| Peptidylprolyl isomerase B | ||
| | | | ||
− | Univeral reference gene in the differentiated fraction | + | *Univeral reference gene in the differentiated fraction |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_022536.1 '''NM_022536.1'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_022536.1 '''NM_022536.1'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
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|align="center"| Peptidyl-prolyl cis–trans isomerase A | |align="center"| Peptidyl-prolyl cis–trans isomerase A | ||
| | | | ||
− | + | *Proliferating intestinal compartments and IEC6 cells. | |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_017101.1 '''NM_017101.1'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_017101.1 '''NM_017101.1'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
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|align="center"| Glyceraldehyde-3-phosphate dehydrogenase | |align="center"| Glyceraldehyde-3-phosphate dehydrogenase | ||
| | | | ||
− | Univeral reference gene in the differentiated fraction | + | *Univeral reference gene in the differentiated fraction |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_017008.4 '''NM_017008.4'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_017008.4 '''NM_017008.4'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 273: | Line 296: | ||
|} | |} | ||
− | === | + | ===Molecular Types=== |
* mRNA | * mRNA | ||
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*'''Name''': Maria Sirakov | *'''Name''': Maria Sirakov | ||
*'''Email''': maria.sirakov@ulb.ac.be | *'''Email''': maria.sirakov@ulb.ac.be | ||
− | *''' | + | *'''Institution''': Laboratoire de Génétique du Développement, Université Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires (IBMM), rue des Profs. Jeener et Brachet 12, 6041 Gosselies,Belgium |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=6555526528695445385&as_sdt=2005&sciodt=0,5&hl=en '''5'''] (Based on Google Scholar [2017-09-01]) | ||
− | =='''Borna Disease | + | =='''''Borna Disease Viral Infection'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 302: | Line 327: | ||
|align="center"| Acidic ribosomal phosphoprotein P0 | |align="center"| Acidic ribosomal phosphoprotein P0 | ||
| | | | ||
− | * Day-9- | + | * Day-9-borna disease virus-infected rat cortical neurons |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_022402 '''NM_022402'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_022402 '''NM_022402'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 312: | Line 337: | ||
|- | |- | ||
|align="center"| ACTB<ref name="ref6"/> | |align="center"| ACTB<ref name="ref6"/> | ||
− | |align="center"| | + | |align="center"| β-actin |
| | | | ||
− | * Day-12- | + | * Day-12-borna disease virus-infected rat cortical neurons |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_031144 '''NM_031144'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_031144 '''NM_031144'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 323: | Line 348: | ||
|align="center"| SYBR | |align="center"| SYBR | ||
|} | |} | ||
− | === | + | |
+ | ===Molecular Types=== | ||
* mRNA | * mRNA | ||
Line 335: | Line 361: | ||
*'''Name''': Peng Xie | *'''Name''': Peng Xie | ||
*'''Email''': xiepeng@cqmu.edu.cn | *'''Email''': xiepeng@cqmu.edu.cn | ||
− | *''' | + | *'''Institution''': Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, China |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=5958914567126320806&as_sdt=2005&sciodt=0,5&hl=en '''10'''] (Based on Google Scholar [2017-09-01]) | ||
− | ==''' | + | =='''''Skeletal Muscle'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 352: | Line 380: | ||
|- | |- | ||
|align="center"| RPL13<ref name="ref7"/> | |align="center"| RPL13<ref name="ref7"/> | ||
− | |align="center"| | + | |align="center"| Ribosomal protein L13 |
| | | | ||
− | skeletal muscle | + | * Contused rat skeletal muscle |
+ | * Fall 150 cm through a clear Lucite guide tube onto the right posterior limb | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_173340 '''NM_173340 '''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_173340 '''NM_173340 '''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 364: | Line 393: | ||
|- | |- | ||
|align="center"| RPL32<ref name="ref7"/> | |align="center"| RPL32<ref name="ref7"/> | ||
− | |align="center"| | + | |align="center"| Ribosomal protein L32 |
| | | | ||
− | skeletal muscle | + | * Contused rat skeletal muscle |
+ | * Fall 150 cm through a clear Lucite guide tube onto the right posterior limb | ||
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013226 '''NM_013226'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013226 '''NM_013226'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 376: | Line 406: | ||
|} | |} | ||
− | === | + | ===Molecular Types=== |
* mRNA | * mRNA | ||
Line 387: | Line 417: | ||
*'''Name''': Ying-yuan Wang | *'''Name''': Ying-yuan Wang | ||
*'''Email''': wyy580218@163.com | *'''Email''': wyy580218@163.com | ||
− | *''' | + | *'''Institution''': Department of Forensic Pathology, Shanxi Medical University, 56 South Xinjian Road, Taiyuan 030001, Shanxi, People’s Republic of China |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=16823738584799176214&as_sdt=2005&sciodt=0,5&hl=en '''35'''] (Based on Google Scholar [2017-09-01]) | ||
− | ==''' | + | =='''''Ischemia & Reperfusion Model'''''== |
− | === | + | ===Internal Control Genes=== |
{|class="wikitable sortable" style="font-size:10pt; width:100%" | {|class="wikitable sortable" style="font-size:10pt; width:100%" | ||
|- | |- | ||
− | ! Gene | + | ! Gene Symbol |
− | ! Gene | + | ! Gene Name |
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
− | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Line 405: | Line 437: | ||
|align="center"| Hydroxymethylbilane synthase | |align="center"| Hydroxymethylbilane synthase | ||
| | | | ||
− | * | + | * Right ventricle and Remote region |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013168 '''NM_013168'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_013168 '''NM_013168'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 417: | Line 449: | ||
|align="center"| Phosphoribosyltransferase 1 | |align="center"| Phosphoribosyltransferase 1 | ||
| | | | ||
− | * | + | * Right ventricle and Remote region |
− | |align="center"| https://www.ncbi.nlm.nih.gov/nuccore/NM_012583 '''NM_012583'''] | + | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_012583 '''NM_012583'''] |
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
* F:CCCAGCGTCGTGATTAGTGATG | * F:CCCAGCGTCGTGATTAGTGATG | ||
Line 429: | Line 461: | ||
|align="center"| TATA binding box protein | |align="center"| TATA binding box protein | ||
| | | | ||
− | * | + | * Right ventricle and Remote region |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_001004198 '''NM_001004198'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_001004198 '''NM_001004198'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
Line 439: | Line 471: | ||
|} | |} | ||
− | === | + | ===Molecular Types=== |
* mRNA | * mRNA | ||
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*'''Name''': Nicoletta Vesentini | *'''Name''': Nicoletta Vesentini | ||
*'''Email''': nvesenti@ifc.cnr.it | *'''Email''': nvesenti@ifc.cnr.it | ||
− | *''' | + | *'''Institution''': Istituto di Fisiologia Clinica, Consiglio Nazionale delle Ricerche, Pisa, Italy Full list of author information is available at the end of the article |
+ | ===Citation Statistics=== | ||
+ | Cited by [https://scholar.google.com/scholar?cites=18111341012430965555&as_sdt=2005&sciodt=0,5&hl=en '''15'''] (Based on Google Scholar [2017-09-01]) | ||
=='''References'''== | =='''References'''== | ||
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</ref> | </ref> | ||
</references> | </references> | ||
+ | |||
+ | =='''Categories'''== | ||
+ | [[Category:Animals]] | ||
+ | [[Category:mRNA]] | ||
+ | [[Category:SYBR]] | ||
+ | [[Category:TaqMan]] | ||
+ | [[Category:ACT]] [[Category:ACT]] [[Category:ACT]] [[Category:ARBP]] [[Category:Cyclophilin]] [[Category:GAPDH]] [[Category:GAPDH]] [[Category:GAPDH]] [[Category:GAPDH]] [[Category:HMBS]] [[Category:HPRT]] [[Category:PGK]] [[Category:RPL]] [[Category:RPL]] [[Category:RPL]] [[Category:SDHA]] [[Category:TBP]] [[Category:YWHAZ]] | ||
+ | [[Category:Specific Tissue]] [[Category:Animal Model]] [[Category:Viral Infection]] [[Category:Cortical Neurons]] [[Category:Specific Cells]] [[Category:Skeletal Muscle]] [[Category:Biotic Stress]] [[Category:Pathological conditions]] | ||
+ | {{Template:BackToTop}} | ||
+ | [[Category:geNorm]] | ||
+ | [[Category:NormFinder]] | ||
+ | [[Category:BestKeeper]] | ||
+ | [[Category:Delta Ct]] | ||
+ | [[Category:RefFinder]] |
Latest revision as of 14:20, 7 September 2017
Contents
Description
- Rattus norvegicus is one of the best known and most common rats. Thought to have originated in northern China, this rodent has now spread to all continents except Antarctica, and is the dominant rat in Europe and much of North America—making it by at least this particular definition the most successful mammal on the planet alongside humans (Modified from Wikipedia).
- Common Name: Rat, Brown rat
- NCBI Taxonomy
Eerebral Ischaemia Model
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
Rpl13a[1] | Ribosomal Protein L13A |
|
NM_173340 |
|
NA | 60 | TaqMan |
Sdha[1] | Succinate dehydrogenase complex, subunit A |
|
NM_130428.1 |
|
NA | 60 | TaqMan |
Ywhaz[1] | Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide |
|
NM_013011.3 |
|
NA | 60 | TaqMan |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Judith Mallolas
- Email: jmallolas.girona.ics@gencat.cat
- Institution: Servei de Neurologia, Fundació Privada Institut d'Investigació Biomèdica de Girona Dr. Josep Trueta (IdIBGi), Hospital Universitari de Girona Dr. Josep Trueta, Girona, Spain
Citation Statistics
Cited by 82 (Based on Google Scholar [2017-09-01])
Asphyxial Cardiac Arrest Model
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
CypA[2] | Cyclophilin A |
|
M19533 |
|
126 | 86 | SYBR |
Pgk1[2] | Phosphoglycerate kinase 1 |
|
NM_053291 |
|
104 | 81.9 | SYBR |
Gapdh[2] | Glyceraldehyde-3-phosphate dehydrogenase |
|
NM_017008 |
|
118 | 82.9 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Kristina Langnaese
- Email: kristina.langnaese@med.ovgu.de
- Institution: nstitute of Medical Neurobiology, University of Magdeburg, Leipziger Str. 44, D-39120 Magdeburg, Germany
Citation Statistics
Cited by 84 (Based on Google Scholar [2017-09-01])
Cardiosphere-derived Cells
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
GAPDH[3] | Glyceraldehyde-3-phosphate dehydrogenase |
|
NM_017008 |
|
NA | 60 | SYBR |
ACTB[3] | β-actin |
|
NM_031144 |
|
NA | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Carolyn A. Carr
- Email: carolyn.carr@dpag.ox.ac.uk
- Institution: Cardiac Metabolism Research Group, Department of Physiology, Anatomy & Genetics, University of Oxford, Sherrington Building, Parks Road, Oxford OX1 3PT, UK
Citation Statistics
Cited by 54 (Based on Google Scholar [2017-08-10])
Adipose Tissue
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
GAPDH[4] | Glyceraldehyde-3-phosphate dehydrogenase |
|
NM_017008.4 |
|
129 | 60 | SYBR |
ACTB[4] | β-actin |
|
NM_031144.3 |
|
150 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
Contact
- Name: You-E Yan
- Email: yanyoue@whu.edu.cn
- Institution: Department of Pharmacology, Basic Medical School of Wuhan University, 185, DongHu Road, Wuhan 430071, China
Citation Statistics
Cited by 8 (Based on Google Scholar [2017-09-01])
Intestinal Epithelial Cells
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
Ppib[5] | Peptidylprolyl isomerase B |
|
NM_022536.1 |
|
115 | 60 | SYBR |
Ppia[5] | Peptidyl-prolyl cis–trans isomerase A |
|
NM_017101.1 |
|
127 | 60 | SYBR |
Gapdh[5] | Glyceraldehyde-3-phosphate dehydrogenase |
|
NM_017008.4 |
|
223 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Maria Sirakov
- Email: maria.sirakov@ulb.ac.be
- Institution: Laboratoire de Génétique du Développement, Université Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires (IBMM), rue des Profs. Jeener et Brachet 12, 6041 Gosselies,Belgium
Citation Statistics
Cited by 5 (Based on Google Scholar [2017-09-01])
Borna Disease Viral Infection
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
ARBP[6] | Acidic ribosomal phosphoprotein P0 |
|
NM_022402 |
|
137 | 60 | SYBR |
ACTB[6] | β-actin |
|
NM_031144 |
|
115 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
- Comparative ΔCt method && Related Reference
Contact
- Name: Peng Xie
- Email: xiepeng@cqmu.edu.cn
- Institution: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, China
Citation Statistics
Cited by 10 (Based on Google Scholar [2017-09-01])
Skeletal Muscle
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
RPL13[7] | Ribosomal protein L13 |
|
NM_173340 |
|
110 | 60 | SYBR |
RPL32[7] | Ribosomal protein L32 |
|
NM_013226 |
|
83 | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
Contact
- Name: Ying-yuan Wang
- Email: wyy580218@163.com
- Institution: Department of Forensic Pathology, Shanxi Medical University, 56 South Xinjian Road, Taiyuan 030001, Shanxi, People’s Republic of China
Citation Statistics
Cited by 35 (Based on Google Scholar [2017-09-01])
Ischemia & Reperfusion Model
Internal Control Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
hmbs[8] | Hydroxymethylbilane synthase |
|
NM_013168 |
|
76 | 60 | SYBR |
hprt[8] | Phosphoribosyltransferase 1 |
|
NM_012583 |
|
110 | 59 | SYBR |
tbp[8] | TATA binding box protein |
|
NM_001004198 |
|
124 | 58 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Nicoletta Vesentini
- Email: nvesenti@ifc.cnr.it
- Institution: Istituto di Fisiologia Clinica, Consiglio Nazionale delle Ricerche, Pisa, Italy Full list of author information is available at the end of the article
Citation Statistics
Cited by 15 (Based on Google Scholar [2017-09-01])
References
- ↑ 1.0 1.1 1.2 Gubern C, Hurtado O, Rodríguez R, et al. Validation of housekeeping genes for quantitative real-time PCR in in-vivo and in-vitro models of cerebral ischaemia[J]. BMC molecular biology, 2009, 10(1): 57.
- ↑ 2.0 2.1 2.2 Langnaese K, John R, Schweizer H, et al. Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model[J]. BMC molecular biology, 2008, 9(1): 53.
- ↑ 3.0 3.1 Tan S C, Carr C A, Yeoh K K, et al. Identification of valid housekeeping genes for quantitative RT-PCR analysis of cardiosphere-derived cells preconditioned under hypoxia or with prolyl-4-hydroxylase inhibitors[J]. Molecular biology reports, 2012, 39(4): 4857-4867.
- ↑ 4.0 4.1 Zhang W X, Fan J, Ma J, et al. Selection of suitable reference genes for quantitative real-time PCR normalization in three types of rat adipose tissue[J]. International Journal of Molecular Sciences, 2016, 17(6): 968.
- ↑ 5.0 5.1 5.2 Sirakov M, Borra M, Cambuli F M, et al. Defining suitable reference genes for RT-qPCR analysis on intestinal epithelial cells[J]. Molecular biotechnology, 2013, 54(3): 930-938.
- ↑ 6.0 6.1 Zhang L, Liu S, Zhang L, et al. Real-time qPCR identifies suitable reference genes for Borna disease virus-infected rat cortical neurons[J]. International journal of molecular sciences, 2014, 15(12): 21825-21839.
- ↑ 7.0 7.1 Sun J, Nan L, Gao C, et al. Validation of reference genes for estimating wound age in contused rat skeletal muscle by quantitative real-time PCR[J]. International journal of legal medicine, 2012, 126(1): 113-120.
- ↑ 8.0 8.1 8.2 Vesentini N, Barsanti C, Martino A, et al. Selection of reference genes in different myocardial regions of an in vivo ischemia/reperfusion rat model for normalization of antioxidant gene expression[J]. BMC research notes, 2012, 5(1): 124.
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