Rattus norvegicus

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Eerebral Ischaemia Model

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
Rpl13a[1] Ribosomal Protein L13A
  • In-vitro model of cerebral ischaemia
 NM_173340
  • F:Rn00821946_g1
  • R:Rn00821946_g1
 NA 60 TaqMan
Sdha[1] Succinate dehydrogenase complex, subunit A
  • In-vivo and in-vitro models of cerebral ischaemia
 NM_130428.1
  • F:Rn00590475_m1
  • R:Rn00590475_m1
 NA 60 TaqMan
Ywhaz[1] Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
  • In-vivo model of cerebral ischaemia
 NM_013011.3
  • F:Rn00755072_m1
  • R:Rn00755072_m1
 NA 60 TaqMan

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Judith Mallolas
  • Email: jmallolas.girona.ics@gencat.cat
  • Institute: Servei de Neurologia, Fundació Privada Institut d'Investigació Biomèdica de Girona Dr. Josep Trueta (IdIBGi), Hospital Universitari de Girona Dr. Josep Trueta, Girona, Spain

Citation Statistics

Cited by 79 (Based on Google Scholar [2017-06-16])

Asphyxial Cardiac Arrest Model

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
CypA[2] cyclophilin A
  • 4 and 21 days post injury
  • 7 days post treatment
 M19533
  • F:TATCTGCACTGCCAAGACTGAGTG
  • R:CTTCTTGCTGGTCTTGCCATTCC
 126 86 SYBR
Pgk1[2] phosphoglycerate kinase 1
  • 4 and 21 days post injury
 NM_053291
  • F:ATGCAAAGACTGGCCAAGCTAC
  • R:AGCCACAGCCTCAGCATATTTC
 104 81.9 SYBR
Gapdh[2] glyceraldehyde-3-phosphate dehydrogenase
  • 7 days post injury
 NM_017008
  • F:CAACTCCCTCAAGATTGTCAGCAA
  • R:GGCATGGACTGTGGTCATGA
 118 82.9 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Kristina Langnaese
  • Email: kristina.langnaese@med.ovgu.de
  • Institute: nstitute of Medical Neurobiology, University of Magdeburg, Leipziger Str. 44, D-39120 Magdeburg, Germany

Citation Statistics

Cited by 81 (Based on Google Scholar [2017-06-16])

Cardiosphere-derived Cells

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
GAPDH[3] glyceraldehyde-3-phosphate dehy-drogenase
  • Neonatal and adult rat CDCs preconditioned by hypoxia or PHDIs
 NM_017008
  • F:GGGTGTGAACCACGAGAAAT
  • R:
 NA 60 SYBR
Actb[3] Beta-actin
  • Neonatal and adult rat CDCs preconditioned by hypoxia or PHDIs
 NM_031144
  • F:CTAAGGCCAACCGTGAAAAG
  • R:AACACAGCCTGGATGGCTAC
 NA 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Carolyn A. Carr
  • Email: carolyn.carr@dpag.ox.ac.uk
  • Institute: Cardiac Metabolism Research Group, Department of Physiology, Anatomy & Genetics, University of Oxford, Sherrington Building, Parks Road, Oxford OX1 3PT, UK

Citation Statistics

Cited by 50 (Based on Google Scholar [2017-06-16])

Rat Adipose Tissue

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
GAPDH[4] Glyceraldehyde-3-phosphate dehydrogenase
  • eWAT Types of Rat Adipose Tissue
 NM_017008.4
  • F:TGCCACTCAGAAGACTGTGG
  • R:TTCAGCTCTGGGATGACCTT
 129 60 SYBR
β-actin[4] β-actin
  • iBeAT & BAT Types of Rat Adipose Tissue
 NM_031144.3
  • F:ATGGATGACGATATCGCTGC
  • R:CTTCTGACCCATACCCACCA
 150 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: You-E Yan
  • Email: yanyoue@whu.edu.cn
  • Institute: Department of Pharmacology, Basic Medical School of Wuhan University, 185, DongHu Road, Wuhan 430071, China

Citation Statistics

Cited by 6 (Based on Google Scholar [2017-06-16])

Intestinal Epithelial Cells

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
Ppib[5] Peptidylprolyl isomerase B
  • Univeral reference gene in the differentiated fraction
 NM_022536.1
  • F:CTGTCGATTCCCTCACAGGT
  • R:AAAATCAGGCCTGTGGAATG
 115 60 SYBR
Ppia[5] Peptidyl-prolyl cis–trans isomerase A
  • Proliferating intestinal compartments and IEC6 cells.
 NM_017101.1
  • F:AGCATACAGGTCCTGGCATC
  • R:TTCACCTTCCCAAAGACCAC
 127 60 SYBR
Gapdh[5] Glyceraldehyde-3-phosphate dehydrogenase
  • Univeral reference gene in the differentiated fraction
 NM_017008.4
  • F:GGCATTGCTCTCAATGACAA
  • R:TGTGAGGGAGATGCTCAGTG
 223 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Maria Sirakov
  • Email: maria.sirakov@ulb.ac.be
  • Institute: Laboratoire de Génétique du Développement, Université Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires (IBMM), rue des Profs. Jeener et Brachet 12, 6041 Gosselies,Belgium

Citation Statistics

Cited by 4 (Based on Google Scholar [2017-06-16])

Borna Disease Virus-Infected Rat Cortical Neurons

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
ARBP[6] Acidic ribosomal phosphoprotein P0
  • Day-9-Borna Disease Virus-Infected Rat Cortical Neurons
 NM_022402
  • F:TAGAGGGTGTCCGCAATGTG
  • R:CAGTGGGAAGGTGTAGTCAGTC
 137 60 SYBR
ACTB[6] Beta-actin
  • Day-12-Borna Disease Virus-Infected Rat Cortical Neurons
 NM_031144
  • F:CAGGGTGTGATGGTGGGTATGG
  • R:AGTTGGTGACAATGCCGTGTTC
 115 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Peng Xie
  • Email: xiepeng@cqmu.edu.cn
  • Institute: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402460, China

Citation Statistics

Cited by 10 (Based on Google Scholar [2017-06-16])

Skeletal Muscle

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
RPL13[7] ribosomal protein L13
  • Contused rat skeletal muscle
 NM_173340
  • F:AAGGTGGTGGTTGTACGCTGTG
  • R:CGAGACGGGTTGGTGTTCATCC
 110 60 SYBR
RPL32[7] ribosomal protein L32
  • Contused rat skeletal muscle
 NM_013226
  • F:CACAGCTGGCCATCAGAGTCA
  • R:AAACAGGCACACCAAGCCATCTATTC
 83 60 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Ying-yuan Wang
  • Email: wyy580218@163.com
  • Institute: Department of Forensic Pathology, Shanxi Medical University, 56 South Xinjian Road, Taiyuan 030001, Shanxi, People’s Republic of China

Citation Statistics

Cited by 34 (Based on Google Scholar [2017-06-16])

In Vivo Ischemia/Reperfusion Model

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primer Size [bp] Tm [℃] Detection
hmbs[8] Hydroxymethylbilane synthase
  • Right ventricle and Remote region
 NM_013168
  • F:TCTAGATGGCTCAGATAGCATGCA
  • R:TGGACCATCTTCTTGCTGAACA
 76 60 SYBR
hprt[8] Phosphoribosyltransferase 1
  • Right ventricle and Remote region
 NM_012583
  • F:CCCAGCGTCGTGATTAGTGATG
  • R:TTCAGTCCTGTCCATAATCAGTCC
 110 59 SYBR
tbp[8] TATA binding box protein
  • Right ventricle and Remote region
 NM_001004198
  • F:CACCGTGAATCTTGGCTGTAAAC
  • R:CGCAGTTGTTCGTGGCTCTC
 124 58 SYBR

Moleculer Types

  • mRNA

Evaluation Methods

Contact

  • Name: Nicoletta Vesentini
  • Email: nvesenti@ifc.cnr.it
  • Institute: Istituto di Fisiologia Clinica, Consiglio Nazionale delle Ricerche, Pisa, Italy Full list of author information is available at the end of the article

Citation Statistics

Cited by 14 (Based on Google Scholar [2017-06-16])

References

  1. ↑ 1.0 1.1 1.2 Gubern C, Hurtado O, Rodríguez R, et al. Validation of housekeeping genes for quantitative real-time PCR in in-vivo and in-vitro models of cerebral ischaemia[J]. BMC molecular biology, 2009, 10(1): 57.
  2. ↑ 2.0 2.1 2.2 Langnaese K, John R, Schweizer H, et al. Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model[J]. BMC molecular biology, 2008, 9(1): 53.
  3. ↑ 3.0 3.1 Tan S C, Carr C A, Yeoh K K, et al. Identification of valid housekeeping genes for quantitative RT-PCR analysis of cardiosphere-derived cells preconditioned under hypoxia or with prolyl-4-hydroxylase inhibitors[J]. Molecular biology reports, 2012, 39(4): 4857-4867.
  4. ↑ 4.0 4.1 Zhang W X, Fan J, Ma J, et al. Selection of suitable reference genes for quantitative real-time PCR normalization in three types of rat adipose tissue[J]. International Journal of Molecular Sciences, 2016, 17(6): 968.
  5. ↑ 5.0 5.1 5.2 Sirakov M, Borra M, Cambuli F M, et al. Defining suitable reference genes for RT-qPCR analysis on intestinal epithelial cells[J]. Molecular biotechnology, 2013, 54(3): 930-938.
  6. ↑ 6.0 6.1 Zhang L, Liu S, Zhang L, et al. Real-time qPCR identifies suitable reference genes for Borna disease virus-infected rat cortical neurons[J]. International journal of molecular sciences, 2014, 15(12): 21825-21839.
  7. ↑ 7.0 7.1 Sun J, Nan L, Gao C, et al. Validation of reference genes for estimating wound age in contused rat skeletal muscle by quantitative real-time PCR[J]. International journal of legal medicine, 2012, 126(1): 113-120.
  8. ↑ 8.0 8.1 8.2 Vesentini N, Barsanti C, Martino A, et al. Selection of reference genes in different myocardial regions of an in vivo ischemia/reperfusion rat model for normalization of antioxidant gene expression[J]. BMC research notes, 2012, 5(1): 124.
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