Difference between revisions of "Stipa grandis"

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(Created page with "=='''Description'''== *'''''Stipa grandis''''' P. Smirn. is a C3 perennial bunchgrass that is distributed widely in eastern Eurasian steppes and the middle Eurasian steppe zo...")
 
 
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=='''Description'''==
 
=='''Description'''==
 +
[[File:Stipa grandis-1.png|right|200px|link=Stipa grandis]]
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* '''''Stipa grandis''''' P. Smirn. is a C3 perennial bunchgrass that is distributed widely in eastern Eurasian steppes and the middle Eurasian steppe zone <ref name="ref1"/><ref name="ref2"/>. This species is dominant in the typical steppe of the Xilingole Plateau of Inner Mongolia <ref name="ref1"/>. The ''S. grandis'' steppe acts as a natural green barrier and plays an important role in sandstorm prevention <ref name="ref3"/><ref name="ref4"/>.
 +
* <font color=blue>'''Common Name:'''</font> '''Unknown'''
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* [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=408131<font color=blue>'''NCBI Taxonomy'''</font>]
 +
 +
=='''''Abiotic Stress'''''==
 +
===Internal Control Genes===
 +
{|class="wikitable sortable" style="font-size:10pt; width:100%"
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|-
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! Gene Symbol 
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! Gene Name
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! style="width=25%　font-size:9pt　"|Application Scope
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! Accession Number
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! Primers (5'-3')<br>[Forward/Reverse]
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! Size [bp]
 +
! Tm [℃]
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! Detection
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|-
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|align="center"| EF1beta<ref name="ref4"/>
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|align="center"| Elongation factor 1-beta
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|
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*Drought and salt stresses
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*Jasmonic acid treatment
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*All samples
 +
|align="center"| NA
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|nowrap style="font-size:9pt"|
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* F:CCATCAACGAATACGTCCAGAG
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* R:CCCCAGTTCCAAGAATCCACTA
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|align="center"| 97
 +
|align="center"| 60
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|align="center"| SYBR
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|-
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|align="center"| UBC15<ref name="ref4"/>
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|align="center"| Ubiquitin-conjugating enzyme 15
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|
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*Drought and salt stresses
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*High pH stress
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*Wounding stress
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*Abscisic acid treatment
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|align="center"| NA
 +
|nowrap style="font-size:9pt"|
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* F:GACCGCTATGTTAGGAACTGCC
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* R:AGAAGAGCCAAACCAATGCC
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|align="center"| 100
 +
|align="center"| 60
 +
|align="center"| SYBR
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|-
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|align="center"| ACT<ref name="ref4"/>
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|align="center"| Actin-7
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|
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*Heat stress
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|align="center"| NA
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|nowrap style="font-size:9pt"|
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* F:GCATGAAGGTGAAGGTGGTT
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* R:TCATCGTACTCTGCCTTGGA
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|align="center"| 121
 +
|align="center"| 60
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|align="center"| SYBR
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|-
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|align="center"| GAPDH<ref name="ref4"/>
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|align="center"| Glyceraldehyde-3-phosphate dehydrogenase
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|
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*Heat stress
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|align="center"| NA
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|nowrap style="font-size:9pt"|
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* F:GGTATGTCCTTCCGTGTTCC
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* R:CGCCTTGATAGCCTTCTTGA
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|align="center"| 105
 +
|align="center"| 60
 +
|align="center"| SYBR
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|-
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|align="center"| SKIP5<ref name="ref4"/>
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|align="center"| ASK-interacting protein
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|
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*Cold stress
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|align="center"| NA
 +
|nowrap style="font-size:9pt"|
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* F:TGTGAGCAAAACCCTCTGGAT
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* R:TTGAGCGATGAGAATGAGTCATACT
 +
|align="center"| 80
 +
|align="center"| 60
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| UBC17<ref name="ref4"/>
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|align="center"| Ubiquitin-conjugating enzyme E2-17
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|
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*Cold stress
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*Jasmonic acid treatment
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*Salicylic acid treatment
 +
|align="center"| NA
 +
|nowrap style="font-size:9pt"|
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* F:TATCCGTTCAAGCCTCCAAAG
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* R:GGTCGGTAAGTAGTGAGCAGATT
 +
|align="center"| 160
 +
|align="center"| 60
 +
|align="center"| SYBR
 +
|-
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|align="center"| HERC2<ref name="ref4"/>
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|align="center"| HECT domain E3 ubiquitin ligases
 +
|
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*High pH stress
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*Salicylic acid treatment
 +
*All samples
 +
|align="center"| NA
 +
|nowrap style="font-size:9pt"|
 +
* F:ATGGGCTTCGGGAGTTTAGG
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* R:GCTTATTTGGGAGACATAGTGACC
 +
|align="center"| 105
 +
|align="center"| 60
 +
|align="center"| SYBR
 +
|-
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|align="center"| UBC2<ref name="ref4"/>
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|align="center"| Ubiquitin-conjugating enzyme E2 2
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|
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*Wounding stress
 +
|align="center"| NA
 +
|nowrap style="font-size:9pt"|
 +
* F:AACATCACGGTCTGGAACGC
 +
* R:GTTGGTGGCTTGTTGGGATAA
 +
|align="center"| 113
 +
|align="center"| 60
 +
|align="center"| SYBR
 +
|-
 +
|align="center"| CUL4<ref name="ref4"/>
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|align="center"| Cullin-4
 +
|
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*Abscisic acid treatment
 +
|align="center"| NA
 +
|nowrap style="font-size:9pt"|
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* F:GTGGAAGGCGTCTGATGTGG
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* R:GCTTAGCTTTTGTGCGTCGTT
 +
|align="center"| 140
 +
|align="center"| 60
 +
|align="center"| SYBR
 +
|}
 +
 +
===Molecular Types===
 +
* mRNA
  
−
*'''''Stipa grandis''''' P. Smirn. is a C3 perennial bunchgrass that is distributed widely in eastern Eurasian steppes and the middle Eurasian steppe zone.<ref name="ref1"/> <ref name="ref2"/>
+
===Evaluation Methods===
−
*This species is dominant in the typical steppe of the Xilingole Plateau of Inner Mongolia. <ref name="ref1"/>
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* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
−
*The S. grandis steppe acts as a natural green barrier and plays an important role in sandstorm prevention.<ref name="ref3"/><ref name="ref4"/>
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* [https://moma.dk/normfinder-software '''NormFinder method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/15289330 '''Related Reference''']
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* <font color=blue>'''Common Name:'''</font> '''Stipa grandis'''
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* [http://www.gene-quantification.com/bestkeeper.html '''BestKeeper method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/15127793 '''Related Reference''']
−
* [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=408131<font color=blue>'''NCBI Taxonomy'''</font>]
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* '''Ref-Finder method''' && [https://www.ncbi.nlm.nih.gov/pubmed/22290409 '''Related Reference''']
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===Contact===
 +
*'''Name''': Xiangyang Hou
 +
*'''Email''': Houxy16@126.com
 +
*'''Institute''': Key Laboratory of Grassland Resources and Utilization of Ministry of Agriculture, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, China
 +
===Citation Statistics===
 +
Cited by '''0''' (Based on Google Scholar [2017-09-01])
  
 
=='''References'''==
 
=='''References'''==
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</ref>
 
</ref>
 
</references>
 
</references>
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=='''Categories'''==
 +
[[Category:EF1α]][[Category:Ubiquitin]] [[Category:ACT]] [[Category:GAPDH]] [[Category:SKIP]] [[Category:HERC2]] [[Category:CUL4]][[Category:Abiotic Stress]][[Category:Drought Treatment]][[Category:Salinity Treatment]][[Category:Hormone Treatment]][[Category:PH Treatment]][[Category:Heat Treatment]][[Category:Cold Treatment]][[Category:SYBR]]
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[[Category:Plants]][[Category:geNorm]][[Category:NormFinder]][[Category:BestKeeper]][[Category:RefFinder]]

Latest revision as of 05:27, 1 September 2017

Description

Stipa grandis-1.png
  • Stipa grandis P. Smirn. is a C3 perennial bunchgrass that is distributed widely in eastern Eurasian steppes and the middle Eurasian steppe zone [1][2]. This species is dominant in the typical steppe of the Xilingole Plateau of Inner Mongolia [1]. The S. grandis steppe acts as a natural green barrier and plays an important role in sandstorm prevention [3][4].
  • Common Name: Unknown
  • NCBI Taxonomy

Abiotic Stress

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse] 		Size [bp] Tm [℃] Detection
EF1beta[4] Elongation factor 1-beta
  • Drought and salt stresses
  • Jasmonic acid treatment
  • All samples
 NA
  • F:CCATCAACGAATACGTCCAGAG
  • R:CCCCAGTTCCAAGAATCCACTA
 97 60 SYBR
UBC15[4] Ubiquitin-conjugating enzyme 15
  • Drought and salt stresses
  • High pH stress
  • Wounding stress
  • Abscisic acid treatment
 NA
  • F:GACCGCTATGTTAGGAACTGCC
  • R:AGAAGAGCCAAACCAATGCC
 100 60 SYBR
ACT[4] Actin-7
  • Heat stress
 NA
  • F:GCATGAAGGTGAAGGTGGTT
  • R:TCATCGTACTCTGCCTTGGA
 121 60 SYBR
GAPDH[4] Glyceraldehyde-3-phosphate dehydrogenase
  • Heat stress
 NA
  • F:GGTATGTCCTTCCGTGTTCC
  • R:CGCCTTGATAGCCTTCTTGA
 105 60 SYBR
SKIP5[4] ASK-interacting protein
  • Cold stress
 NA
  • F:TGTGAGCAAAACCCTCTGGAT
  • R:TTGAGCGATGAGAATGAGTCATACT
 80 60 SYBR
UBC17[4] Ubiquitin-conjugating enzyme E2-17
  • Cold stress
  • Jasmonic acid treatment
  • Salicylic acid treatment
 NA
  • F:TATCCGTTCAAGCCTCCAAAG
  • R:GGTCGGTAAGTAGTGAGCAGATT
 160 60 SYBR
HERC2[4] HECT domain E3 ubiquitin ligases
  • High pH stress
  • Salicylic acid treatment
  • All samples
 NA
  • F:ATGGGCTTCGGGAGTTTAGG
  • R:GCTTATTTGGGAGACATAGTGACC
 105 60 SYBR
UBC2[4] Ubiquitin-conjugating enzyme E2 2
  • Wounding stress
 NA
  • F:AACATCACGGTCTGGAACGC
  • R:GTTGGTGGCTTGTTGGGATAA
 113 60 SYBR
CUL4[4] Cullin-4
  • Abscisic acid treatment
 NA
  • F:GTGGAAGGCGTCTGATGTGG
  • R:GCTTAGCTTTTGTGCGTCGTT
 140 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Xiangyang Hou
  • Email: Houxy16@126.com
  • Institute: Key Laboratory of Grassland Resources and Utilization of Ministry of Agriculture, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, China

Citation Statistics

Cited by 0 (Based on Google Scholar [2017-09-01])

References

  1. ↑ 1.0 1.1 Wu JB, Gao YB, Bao XY, Gao H, Jia MQ, Li J, Zhao NX. Genetic variation among Stipa grandis P. Smirn populations with different durations of fencing in the Inner Mongolian Steppe. The Rangeland Journal. 2010 Dec 17;32(4):427-34.
  2. ↑ Song X, Wang Y, Lv X. Responses of plant biomass, photosynthesis and lipid peroxidation to warming and precipitation change in two dominant species (Stipa grandis and Leymus chinensis) from North China Grasslands. Ecol Evol. 2016 Feb 20;6(6):1871-82.
  3. ↑ Zhao NX, Gao YB, Wang JL, Ren AZ. Population structure and genetic diversity of Stipa grandis P. Smirn, a dominant species in the typical steppe of northern China. Biochemical Systematics and Ecology. 2008 Jan 31;36(1):1-0.
  4. ↑ 4.0 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 Wan D, Wan Y, Yang Q, Zou B, Ren W, Ding Y, Wang Z, Wang R, Wang K, Hou X. Selection of Reference Genes for qRT-PCR Analysis of Gene Expression in Stipa grandis during Environmental Stresses. PLoS One. 2017 Jan 5;12(1):e0169465.

Categories

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