Description
- Sus scrofa L. is a species that is utilised for food and sport hunting throughout the world. It can be described as a mammal and generalist omnivore, which is able to vary its diet. It has broad geographic distributions that include semi deserts, wetlands, high mountain environments and forest ecosystems. Translocations by humans have spread this species across the world[1][2][3]
- Common Name: Wild boar
- NCBI Taxonomy
Spermatozoa Cryopreservation
Internal Control Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primers (5'-3')
[Forward/Reverse]
|
Size [bp]
|
Tm [℃]
|
Detection
|
| GAPDH[1]
|
Glyceraldehyde 3-phosphate dehydrogenase
|
- Different concentrations of cryoprotectants
|
DQ178124
|
- F:ACTCACTCTTCTACCTTTGATGCT
- R:TGTTGCTGTAGCCAAATTCA
|
100
|
59.0
|
SYBR
|
| RPL4[1]
|
Ribosomal protein L4
|
- Different concentrations of cryoprotectants
|
DQ845176
|
- F:AGGAGGCTGTTCTGCTTCTG
- R:TCCAGGGATGTTTCTGAAGG
|
185
|
60
|
SYBR
|
| PPIA[1]
|
Peptidylprolyl isomerase A
|
- Different concentrations of cryoprotectants
|
NM_214353
|
- F:CACAAACGGTTCCCAGTTTT
- R:TGTCCACAGTCAGCAATGGT
|
171
|
60
|
SYBR
|
Molecular Types
Evaluation Methods
Contact
- Name: Changjun Zeng
- Email: zengchj@sicau.edu.cn
- Institution: College of Animal Science and Technology, Sichuan Agricultural University, Ya’an, Sichuan 625014, PR China
Citation Statistics
Cited by 21 (Based on Google Scholar [2017-09-01])
Actinobacillus pleuropneumoniae Infection
Internal Control Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| GAPDH[2]
|
Glyceraldehyde-3-phosphate dehydrogenase
|
- Tissues and blood infected with Actinobacillus pleuropneumoniae
|
TC162668
|
- F:ACCCAGAAGACTGTGGATGG
- R:AAGCAGGGATGATGTTCTGG
|
NA
|
62
|
AmpliQ
|
Molecular Types
Evaluation Methods
Contact
- Name: Kerstin Skovgaard
- Email: kis@vet.dtu.dk
- Institution: Department of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark, Bu¨lowsvej 27, DK-1790 Copenhagen, Denmark
Citation Statistics
Cited by 31 (Based on Google Scholar [2017-09-01])
Oocytes & Preimplantation Embryos
Internal Control Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| GAPDH[3]
|
Glyceraldehyde 3-phosphate dehydrogenase
|
- Early porcine embryonic development
|
AF017079
|
- F:TCGGAGTGAACGGATTTG
- R:CCTGGAAGATGGTGATGG
|
219
|
51.1
|
SYBR
|
| PGK1[3]
|
Phosphoglycerate kinase 1
|
- Early porcine embryonic development
|
AY677198
|
- F:AGATAACGAACAACCAGAGG
- R:TGTCAGGCATAGGGATACC
|
126
|
56.4
|
SYBR
|
| S18[3]
|
18S ribosomal RNA
|
- Early porcine embryonic development
|
NR_002170
|
- F:GGCTACCACATCCAAGGAAG
- R:TCCAATGGATCCTCGCGGAA
|
149
|
58.7
|
SYBR
|
| UBC[3]
|
Poly-ubiquitin
|
- Early porcine embryonic development
|
M18159
|
- F:TTCGTGAAGACCTTGACTG
- R:GGACTCCTTCTGGATGTTG
|
186
|
51.1
|
SYBR
|
Molecular Types
Evaluation Methods
Contact
- Name: Bernard AJ Roelen
- Email: b.a.j.roelen@vet.uu.nl
- Institution: Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, 3584 CM Utrecht, The Netherlands
Citation Statistics
Cited by 119 (Based on Google Scholar [2017-09-01])
Gastrointestinal Tissues
Internal Control Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| B2M[4]
|
Beta-2-microglobulin
|
- Liver
- Ileum
- Colon
- All treatment groups
- Entire data set
|
NM_213978
|
- F:CGGAAAGCCAAATTACCTGAAC
- R:TCTCCCCGTTTTTCAGCAAAT
|
83
|
59~60
|
SYBR
|
| GAPDH[4]
|
Glyceraldehyde 3-phosphate dehydrogenase
|
- Liver
- All treatment groups
- Entire data set
|
AF017079
|
- F:CAGCAATGCCTCCTGTACCA
- R:ACGATGCCGAAGTTGTCATG
|
72
|
58
|
SYBR
|
| PPIA[4]
|
Peptidylprolyl isomerase A
|
|
NM_214353
|
- F:CGGGTCCTGGCATCTTGT
- R:TGGCAGTGCAAATGAAAAACTG
|
75
|
56.5~58
|
SYBR
|
Molecular Types
Evaluation Methods
Contact
- Name: T. Sweeney
- Email: Marion.Ryan@ucd.ie
- Institution: School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Ireland
Citation Statistics
Cited by 15 (Based on Google Scholar [2017-09-01])
Abiotic Stress
Internal Control Genes
| Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
| RPL4[5]
|
Ribosomal protein L4
|
- 21 consecutive days of heat stress except on day 21
|
DQ845176
|
- F:GCTCTATGGCACTTGGCGT
- R:GCGGAGGGCTCTTTGGAT
|
124
|
55
|
iCycler IQ Real-Time PCR Detection System (Bio-Rad)
|
| TBP[5]
|
TATA-box-binding protein
|
- 21 consecutive days of heat stress except on day 21
|
DQ845178
|
- F:GTAGTTATGAGCCAGAGT
- R:CCTTTAGGATAGGGTAG
|
159
|
55
|
iCycler IQ Real-Time PCR Detection System (Bio-Rad)
|
| B2M[5]
|
Beta-2-microglobulin
|
- 23 consecutive days of heat stress
|
NM_213978
|
- F:TATCTGGGTTCCATCCG
- R:AACTATCTTGGGCTTATCG
|
197
|
55
|
iCycler IQ Real-Time PCR Detection System (Bio-Rad)
|
Molecular types
Evaluation Methods
Contact
- Name: Liao Ming
- Email: mliao@scau.edu.cn
- Institute: The South China Agricultural University, Key Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture, College of Veterinary Medicine, Guangzhou 510642, China
Citation Statistics
Cited by 23 (Based on Google Scholar [2017-09-01])
References
- ↑ 1.0 1.1 1.2 1.3
Zeng C, He L, Peng W, et al. Selection of optimal reference genes for quantitative RT-PCR studies of boar spermatozoa cryopreservation[J]. Cryobiology, 2014, 68(1): 113-121.
- ↑ 2.0 2.1
Skovgaard K, Mortensen S, Poulsen K T, et al. Validation of putative reference genes for qRT-PCR normalization in tissues and blood from pigs infected with Actinobacillus pleuropneumoniae[J]. Veterinary immunology and immunopathology, 2007, 118(1): 140-146.
- ↑ 3.0 3.1 3.2 3.3 3.4
Kuijk E W, du Puy L, van Tol H T A, et al. Validation of reference genes for quantitative RT-PCR studies in porcine oocytes and preimplantation embryos[J]. BMC Developmental Biology, 2007, 7(1): 58.
- ↑ 4.0 4.1 4.2
Ryan M T, Collins C B, O'Doherty J V, et al. Selection of stable reference genes for quantitative real-time PCR in porcine gastrointestinal tissues[J]. Livestock Science, 2010, 133(1): 42-44.
- ↑ 5.0 5.1 5.2
Xiang-Hong J, Yan-Hong Y, Han-Jin X, et al. Selection of reference genes for gene expression studies in PBMC from Bama miniature pig under heat stress[J]. Veterinary immunology and immunopathology, 2011, 144(1): 160-166.
Categories
