Difference between revisions of "Test7"

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=='''Description'''==
 
[[File:Glycine max.png|right|200px|link=Glycine max]]
 
* '''''Glycine max''''' is a species of legume native to East Asia. It is the world's major economically important agricultural crop widely grown for its edible bean. It also provides important source of vegetable oil and proteins and has numerous uses such as biofuel, feedstock, industrial products, and cosmetics. The main countries growing soybeans are the United States, Brazil and Argentina <ref name="ref1"/><ref name="ref2"/>.
 
* <font color=blue>'''Common Name:'''</font> '''Soybean'''
 
* [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=3847 <font color=blue>'''NCBI Taxonomy'''</font>]
 
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=='''Heavy Metals & Heat Treatments'''==
 
 
===Internal Control Genes===
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
|-
 
! Gene Symbol 
 
! Gene Name
 
! style="width=25% font-size:9pt "|Application Scope
 
! Accession Number
 
! Primers (5'-3')<br>[Forward/Reverse]
 
! Size [bp]
 
! Tm [℃]
 
! Detection
 
|-
 
|align="center"| TUA4<ref name="ref1"/>
 
|align="center"| Tubulin alpha-4
 
|
 
* Roots under Al stress
 
* Roots and leaves under heat stress
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/XM_003555953.3 '''LOC100781185''']
 
|nowrap style="font-size:9pt"|
 
* F:CATACCCTAGAATCCATTTC-3
 
* R:TGTACTTTCCGTGACGAG-3
 
|align="center"| 159
 
|align="center"| 60
 
|align="center"| SYBR
 
|-
 
|align="center"| 60SRP<ref name="ref1"/>
 
|align="center"| 60S ribosome protein
 
|
 
* Roots and leaves under Cd stress
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/XM_003551021.3 '''LOC100778077''']
 
|nowrap style="font-size:9pt"|
 
* F:AAAGTGGACCAAGGCATATCGTCG-3
 
* R:TCAGGACATTCTCCGCAAGATTCC-3
 
|align="center"| 125
 
|align="center"| 60
 
|align="center"| SYBR
 
|}
 
 
===Molecular Types===
 
* mRNA
 
 
===Evaluation Methods===
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 
* [https://moma.dk/normfinder-software '''NormFinder method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/15289330 '''Related Reference''']
 
* [http://www.gene-quantification.com/bestkeeper.html '''BestKeeper method'''] && [https://www.ncbi.nlm.nih.gov/pubmed/15127793 '''Related Reference''']
 
* '''Comparative ΔCt method''' && [https://www.ncbi.nlm.nih.gov/pubmed/17026756 '''Related Reference''']
 
*  '''Ref-Finder method''' && [https://www.ncbi.nlm.nih.gov/pubmed/22290409 '''Related Reference''']
 
 
===Contact===
 
*'''Name''': Yan Li
 
*'''Email''': yanli1@njau.edu.cn
 
*'''Institution''': National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, Jiangsu, China
 
===Citation Statistics===
 
Cited by '''1''' (Based on Google Scholar [2017-06-29])
 
 
=='''References'''==
 
<references>
 
<ref name="ref1">
 
Gao M, Liu Y, Ma X, et al. Evaluation of Reference Genes for Normalization of Gene Expression Using Quantitative RT-PCR under Aluminum, Cadmium, and Heat Stresses in Soybean[J]. PloS one, 2017, 12(1): e0168965.
 
</ref>
 
 
<ref name="ref2">
 
Bansal R, Mittapelly P, Cassone B J, et al. Recommended reference genes for quantitative PCR analysis in soybean have variable stabilities during diverse biotic stresses[J]. PloS one, 2015, 10(8): e0134890.
 
</ref>
 
 
</references>
 
 
=='''Categories'''==
 
[[Category:Plants]]
 
[[Category:mRNA]]
 
[[Category:SYBR]]
 
[[Category:60SRP]] [[Category:RPL]] [[Category:ABCT]] [[Category:ABCT]] [[Category:ACT]] [[Category:Cyclophilin]] [[Category:F-box]] [[Category:F-box]] [[Category:F-box]] [[Category:IDE]] [[Category:Tubulin]] [[Category:Tubulin]] [[Category:Tubulin]]
 
[[Category:Salinity Treatment]]  [[Category:Different Tissues]]  [[Category:Abiotic Stress]]  [[Category:Heat Treatment]]  [[Category:Dehydration Treatment]]  [[Category:Biotic Stress]]  [[Category:Hormone Treatment]]  [[Category:Hypoxic Treatment]]  [[Category:Cold Treatment]]  [[Category:Heavy Metals Treatments]]
 
[[Category:geNorm]]
 
[[Category:NormFinder]]
 
[[Category:BestKeeper]]
 
[[Category:Delta Ct]]
 
[[Category:RefFinder]]
 

Revision as of 14:11, 20 August 2017