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Glycine max.png
  • Glycine max is a species of legume native to East Asia. It is the world's major economically important agricultural crop widely grown for its edible bean. It also provides important source of vegetable oil and proteins and has numerous uses such as biofuel, feedstock, industrial products, and cosmetics. The main countries growing soybeans are the United States, Brazil and Argentina [1][2].
  • Common Name: Soybean
  • NCBI Taxonomy

Heavy Metals & Heat Treatments

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
Size [bp] Tm [℃] Detection
TUA4[1] Tubulin alpha-4
  • Roots under Al stress
  • Roots and leaves under heat stress
159 60 SYBR
60SRP[1] 60S ribosome protein
  • Roots and leaves under Cd stress
125 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods


  • Name: Yan Li
  • Email: yanli1@njau.edu.cn
  • Institution: National Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Key Laboratory for Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, Jiangsu, China

Citation Statistics

Cited by 1 (Based on Google Scholar [2017-06-29])


  1. 1.0 1.1 1.2 Gao M, Liu Y, Ma X, et al. Evaluation of Reference Genes for Normalization of Gene Expression Using Quantitative RT-PCR under Aluminum, Cadmium, and Heat Stresses in Soybean[J]. PloS one, 2017, 12(1): e0168965.
  2. Bansal R, Mittapelly P, Cassone B J, et al. Recommended reference genes for quantitative PCR analysis in soybean have variable stabilities during diverse biotic stresses[J]. PloS one, 2015, 10(8): e0134890.