Difference between revisions of "Tribolium castaneum"

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(Created page with "=='''Description'''== =='''''Fungal Challenge'''''== ===Reference Genes=== {|class="wikitable sortable" style="font-size:10pt; width:100%" |- ! Gene Symbol ! Gene Name ! st...")
 
 
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=='''Description'''==
 
=='''Description'''==
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[[File:Tribolium castaneum.png|right|200px|link=Tribolium castaneum]]
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* '''''Tribolium castaneum''''' is a species of beetle in the family Tenebrionidae. It is a worldwide pest of stored products, particularly food grains. It has become an important insect model organism and pest beetle species for ethological and food safety research as well as for studies of developmental genetics, evolutionary developmental biology and insect immunity. Its genome has been fully sequenced<ref name="ref1"/><ref name="ref2"/>.
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* <font color=blue>'''Common Name:'''</font> '''Red flour beetle'''
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* [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=7070 <font color=blue>'''NCBI Taxonomy'''</font>]
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=='''''Fungal Challenge'''''==
 
=='''''Fungal Challenge'''''==
===Reference Genes===
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===Internal Control Genes===
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
{|class="wikitable sortable" style="font-size:10pt; width:100%"
 
|-
 
|-
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! style="width=25% font-size:9pt "|Application Scope  
 
! style="width=25% font-size:9pt "|Application Scope  
 
! Accession Number  
 
! Accession Number  
! Primer
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! Primers (5'-3')<br>[Forward/Reverse]
 
! Size [bp]  
 
! Size [bp]  
 
! Tm [℃]
 
! Tm [℃]
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|align="center"| Ribosomal protein S18
 
|align="center"| Ribosomal protein S18
 
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|
exposed to Beauveria bassiana
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*Exposed to Beauveria bassiana
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/XM_968539 '''XM_968539''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/XM_968539 '''XM_968539''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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|align="center"| Ribosomal protein S3  
 
|align="center"| Ribosomal protein S3  
 
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|
exposed to Beauveria bassiana
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*Exposed to Beauveria bassiana
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_001172392 '''XM_965494''']  
 
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/NM_001172392 '''XM_965494''']  
 
|nowrap style="font-size:9pt"|
 
|nowrap style="font-size:9pt"|
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|align="center"| SYBR
 
|align="center"| SYBR
 
|}
 
|}
===Moleculer Type===
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===Molecular Types===
 
* mRNA
 
* mRNA
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===Evaluation Methods===
 
===Evaluation Methods===
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
 
* [https://genorm.cmgg.be/ '''geNorm method''']  && [https://www.ncbi.nlm.nih.gov/pubmed/12184808 '''Related Reference''']
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*'''Email''': jeff.lord@ars.usda.gov
 
*'''Email''': jeff.lord@ars.usda.gov
 
*'''Institution''': USDA-ARS, Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA
 
*'''Institution''': USDA-ARS, Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA
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===Citation Statistics===
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Cited by [https://scholar.google.com/scholar?cites=9658128174012942111&as_sdt=2005&sciodt=0,5&hl=en '''74'''] (Based on Google Scholar [2017-09-01])
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=='''References'''==
 
=='''References'''==
 
<references>
 
<references>
 
<ref name="ref1">
 
<ref name="ref1">
 
Lord J C, Hartzer K, Toutges M, et al. Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge[J]. Journal of microbiological methods, 2010, 80(2): 219-221.
 
Lord J C, Hartzer K, Toutges M, et al. Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge[J]. Journal of microbiological methods, 2010, 80(2): 219-221.
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</ref>
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<ref name="ref2">
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Strobl F, Klees S, Stelzer EHK.J Vis Exp. 2017 Apr 28;(122). doi: 10.3791/55629.PMID: 28518097.
 
</ref>
 
</ref>
 
</references>
 
</references>
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=='''Categories'''==
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[[Category:Animals]] [[Category:mRNA]] [[Category:SYBR]]
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[[Category:RPS]] [[Category:RPS]]
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[[Category:Fungal infection]]  [[Category:Biotic Stress]]  [[Category:Pathological conditions]]
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[[Category:geNorm]]
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[[Category:NormFinder]]

Latest revision as of 04:52, 1 September 2017

Description

Tribolium castaneum.png
  • Tribolium castaneum is a species of beetle in the family Tenebrionidae. It is a worldwide pest of stored products, particularly food grains. It has become an important insect model organism and pest beetle species for ethological and food safety research as well as for studies of developmental genetics, evolutionary developmental biology and insect immunity. Its genome has been fully sequenced[1][2].
  • Common Name: Red flour beetle
  • NCBI Taxonomy

Fungal Challenge

Internal Control Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
[Forward/Reverse]
Size [bp] Tm [℃] Detection
RPS18[1] Ribosomal protein S18
  • Exposed to Beauveria bassiana
XM_968539
  • F:CGAAGAGGTCGAGAAAATCG
  • R:CGTGGTCTTGGTGTGTTGAC
NA 55 SYBR
RPS3[1] Ribosomal protein S3
  • Exposed to Beauveria bassiana
XM_965494
  • F:ACCTCGATACACCATAGCAAGC
  • R:ACCGTCGTATTCGTGAATTGAC
NA 55 SYBR

Molecular Types

  • mRNA

Evaluation Methods

Contact

  • Name: Brenda Oppert
  • Email: jeff.lord@ars.usda.gov
  • Institution: USDA-ARS, Grain Marketing and Production Research Center, 1515 College Avenue, Manhattan, KS 66502, USA

Citation Statistics

Cited by 74 (Based on Google Scholar [2017-09-01])

References

  1. 1.0 1.1 1.2 Lord J C, Hartzer K, Toutges M, et al. Evaluation of quantitative PCR reference genes for gene expression studies in Tribolium castaneum after fungal challenge[J]. Journal of microbiological methods, 2010, 80(2): 219-221.
  2. Strobl F, Klees S, Stelzer EHK.J Vis Exp. 2017 Apr 28;(122). doi: 10.3791/55629.PMID: 28518097.

Categories