Trifolium pratense

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Trifolium pratense-1.jpg
  • Red clover (Trifolium pratense L.) is an important forage legume widely cultivated in most temperate regions because of its characteristics of high seedling vigor, rapid growth, and tolerance to acidic and humid conditions.
  • It is also used as a green manure crop because of its high nutrient content resulting partly from symbiosis with nitrogen fixing bacteria of the genus Rhizobium. Red clover has a diploid genome (2n = 2x = 14), with a DNA content of 0.97 pg/2C,7 which is slightly larger than that of rice[1] [2].
  • Common Name: Red clover
  • NCBI Taxonomy

Different Tissues

Reference Genes

Gene Symbol Gene Name Application Scope Accession Number Primers (5'-3')
Size [bp] Tm [℃] Detection
YLS8[1] Yellow-leaf-specific protein 8
  • Universal reference gene
93 60 SYBR
UBC2[1] Ubiquitin-conjugating enzyme E2
  • Universal reference gene
97 60 SYBR

Molecular Types

  • mRNA

Evaluation Methods


  • Name: Khosro Mehdi Khanlou
  • Email:
  • Institution: Department of Plant Production, Ghent University, Coupure Links 653, 9000 Ghent, Belgium

Citation Statistics

Cited by 28 (Based on Google Scholar [2017-06-01])


  1. 1.0 1.1 1.2 Khanlou K M, Van Bockstaele E. A critique of widely used normalization software tools and an alternative method to identify reliable reference genes in red clover (Trifolium pratense L.)[J]. Planta, 2012, 236(5): 1381-1393.
  2. Sato S, Isobe S, Asamizu E, et al. Comprehensive structural analysis of the genome of red clover (Trifolium pratense L.)[J]. DNA research, 2005, 12(5): 301-364.