Ulva linza
Contents
Description
- U. linza is a green macroalga (seaweed) that is widely used as a model organism in marine biofouling studies. Reproduction is facilitated by the release of motile, pear-shaped zoospores, 5每8 米m in length that are surrounded by a lipoprotein plasma membrane, ie there is no polysaccharide cell wall. Spores have a negative zeta potential, are negatively phototactic and also respond to a number of surface cues eg wettability, charge and topography. Settlement involves rapid secretion of a glycoprotein adhesive and permanent attachment to the substratum, retraction of the four flagella resulting in loss of motility, and the formation of a polysaccharide-rich cell wall. Permanently attached zoospores are rounded and typically 4-5 μm in diameter. Settlement (ie permanent attachment) is followed by germination of the attached spore, cell division and eventually the formation of a new adult plant[1] [2] .
Different Experimental Conditions
Reference Genes
Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
---|---|---|---|---|---|---|---|
TUB2[1] | Tubulin2 |
|
JN543389 |
|
NA | 60 | SYBR |
H2[1] | Histone 2 |
|
JN543390 |
|
NA | 60 | SYBR |
18S[1] | 18S rRNA |
|
HM584731 |
|
NA | 60 | SYBR |
Molecular Types
- mRNA
Evaluation Methods
Contact
- Name: Meitao Dong
- Email: yenh@ysfri.ac.cn
- Institution: Qingdao Agricultural University, Qingdao 266109, China
Citation Statistics
Cited by 19 (Based on Google Scholar [2017-06-16])
References
- ↑ 1.0 1.1 1.2 1.3 Dong M, Zhang X, Chi X, et al. The validity of a reference gene is highly dependent on the experimental conditions in green alga Ulva linza[J]. Current genetics, 2012, 58(1): 13-20.
- ↑ Heydt M, Div車s P, Grunze M, Rosenhahn A. 2009. Analysis of holographic microscopy data to quantitatively investigate three-dimensional settlement dynamics of algal zoospores in the vicinity of surfaces. Eur Phys J E. 30:141-148.