Vigna mungo

Description

Cultivated blackgram (Vigna mungo) is an annual, selfpollinated grain legume consumed for its high-quality edible protein throughout the world. Besides its use in global cuisine, it is also an eminent restorer of soil fertility through biological nitrogen fixation. Substantial progress has already been made in V. mungo breeding; the present effort is now directed toward integration of genomics in crop improvement.
Moreover, rapid advancement in legume genome sequencing coupled with high-throughput expression analysis has resulted in mining of agronomically vital genes, implicating their deployment in the development of superior cultivars. All these studies often need an efficient tool such as qPCR to analyze the expression levels of some responsive genes to develop logistic improvement of the crop^[1] ^[2].

Gene Symbol	Gene Name	Application Scope	Accession Number	Primer	Size [bp]	Tm [℃]	Detection
ACT^[1]	actin	Mungbean yellow mosaic India virus infection Salinity stress	JZ078743	F:GTTCTGTTCCAGCCATCCAT R:GTGGTGCGACAACCTTGATT	220	83.5	SYBR
EF1A^[1]	Elongation factor 1-alpha	Mungbean yellow mosaic India virus infection	HO223992	F:CCTCTTGGACGTTTTGCTGT R:TGTAACCATCCCCTGATGA	157	85.5	SYBR
H2A^[1]	histone H2A	Drought treatments	JG016051	F:GATGACACCACCACCAACAA R:GCTGGGAATGCAAGTAAGGA	126	83.0	SYBR
18S^[1]	18S rRNA	Drought treatments	JZ078742	F:CTCTGCCTGTTGCTTTGATG R:CTCTGCCTGTTGCTTTGATG	152	85.5	SYBR

Name: Amita Pal
Email: amita_pal@yahoo.com
Institution: Division of Plant Biology, Bose Institute, Kolkata 700054,West Bengal, India

Cited by 23 (Based on Google Scholar [2017-06-16])

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 Kundu A, Patel A, Pal A. Defining reference genes for qPCR normalization to study biotic and abiotic stress responses in Vigna mungo[J]. Plant cell reports, 2013, 32(10): 1647-1658.
↑ Basak J, Kundagramy S, Ghose TK, Pal A (2005) Development of yellow mosaic virus (YMV) resistance linked DNA-marker in Vigna mungo from population segregating for YMV reaction. Mol Breed 14:375–383