Difference between revisions of "Vitis vinifera"
Jump to navigation
Jump to search
ICG Expert4 (talk | contribs) |
|||
| Line 9: | Line 9: | ||
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
| − | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
| Line 20: | Line 20: | ||
* Biotic stress effect | * Biotic stress effect | ||
* Resistant cultivar regent | * Resistant cultivar regent | ||
| − | * Downy | + | * Downy mildew infection |
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/EC922622 '''EC922622'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nucest/EC922622 '''EC922622'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
| Line 35: | Line 35: | ||
* Susceptible cultivar trincadeira | * Susceptible cultivar trincadeira | ||
* Resistant cultivar regent | * Resistant cultivar regent | ||
| − | * Downy | + | * Downy mildew infection |
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/EC959059 '''EC959059'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nucest/EC959059 '''EC959059'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
| Line 49: | Line 49: | ||
* Genotype effect | * Genotype effect | ||
* Resistant cultivar regent | * Resistant cultivar regent | ||
| − | * Downy | + | * Downy mildew infection |
|align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/EF192466 '''EF192466'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nuccore/EF192466 '''EF192466'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
| Line 62: | Line 62: | ||
| | | | ||
* Susceptible cultivar trincadeira | * Susceptible cultivar trincadeira | ||
| − | * Downy | + | * Downy mildew infection |
|align="center"| [https://www.ncbi.nlm.nih.gov/nucest/CF405409 '''CF405409'''] | |align="center"| [https://www.ncbi.nlm.nih.gov/nucest/CF405409 '''CF405409'''] | ||
|nowrap style="font-size:9pt"| | |nowrap style="font-size:9pt"| | ||
| Line 96: | Line 96: | ||
! style="width=25% font-size:9pt "|Application Scope | ! style="width=25% font-size:9pt "|Application Scope | ||
! Accession Number | ! Accession Number | ||
| − | ! | + | ! Primers (5'-3')<br>[Forward/Reverse] |
! Size [bp] | ! Size [bp] | ||
! Tm [℃] | ! Tm [℃] | ||
Revision as of 09:04, 20 June 2017
Contents
Description
- Grapevine is one of the most cultivated fruit crop worldwide with Vitis vinifera being the species with the highest economical importance due to the high quality standards of its berries.
Mycotic Infection
Reference Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| UBQ[1] | Ubiquitin-conjugating enzyme |
|
EC922622 |
|
75 | 60 | SYBR |
| EF1a[1] | Elongation factor 1a |
|
EC959059 |
|
164 | 60 | SYBR |
| GAPDH[1] | glyceraldehyde-3-phosphate dehy-drogenase |
|
EF192466 |
|
226 | 60 | SYBR |
| SAND[1] | SAND family protein |
|
CF405409 |
|
76 | 60 | SYBR |
Moleculer Type
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
Contact
- Name: Filipa Monteiro
- Email: fimonteiro@fc.ul.pt
- Institute: Plant Systems Biology Lab, Center of Biodiversity, Functional & Integrative Genomics (BioFIG), Science Faculty of Lisbon University, Lisboa, Portugal
Citation Statistics
Cited by 29 (Based on Google Scholar [2017-06-16])
Pterostilbene Synthesis
Reference Genes
| Gene Symbol | Gene Name | Application Scope | Accession Number | Primers (5'-3') [Forward/Reverse] |
Size [bp] | Tm [℃] | Detection |
|---|---|---|---|---|---|---|---|
| VATP16[2] | V-type proton ATPase 16 kDa proteolipid subunit |
|
XM_002269086 |
|
112 | 85.23 | SYBR |
| 60SRP[2] | 60S ribosomal protein L18 |
|
XM_002270599 |
|
165 | 82.39 | SYBR |
Moleculer types
- mRNA
Evaluation Methods
- geNorm method && Related Reference
- NormFinder method && Related Reference
- BestKeeper method && Related Reference
Contact
- Name: Marielle Adrian
- Email: marielle.adrian@u-bourgogne.fr
- Institute: Unité Mixte de Recherche INRA 1088/CNRS 5184, Université de Bourgogne Plante-Microbe-Environnement, 17 rue Sully, BP 86510, 21065 Dijon cedex, France
References
- ↑ 1.0 1.1 1.2 1.3 Monteiro F, Sebastiana M, Pais M S, et al. Reference gene selection and validation for the early responses to downy mildew infection in susceptible and resistant Vitis vinifera cultivars[J]. PloS one, 2013, 8(9): e72998.
- ↑ 2.0 2.1 Gamm M, Héloir M C, Kelloniemi J, et al. Identification of reference genes suitable for qRT-PCR in grapevine and application for the study of the expression of genes involved in pterostilbene synthesis[J]. Molecular Genetics and Genomics, 2011, 285(4): 273-285.
