Description
- Grapevine is one of the most cultivated fruit crop worldwide with Vitis vinifera being the species with the highest economical importance due to the high quality standards of its berries.
Mycotic Infection
Reference Genes
Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
UBQ[1]
|
Ubiquitin-conjugating enzyme
|
- Genotype effect
- Biotic stress effect
- Resistant cultivar regent
- Downy Mildew Infection
|
EC922622
|
- F:GAGGGTCGTCAGGATTTGGA
- R:GCCCTGCACTTACCATCTTTAAG
|
75
|
60
|
SYBR
|
EF1a[1]
|
Elongation factor 1a
|
- Genotype effect
- Susceptible cultivar trincadeira
- Resistant cultivar regent
- Downy Mildew Infection
|
EC959059
|
- F:GAACTGGGTGCTTGATAGGC
- R:ACCAAAATATCCGGAGTAAAAGA
|
164
|
60
|
SYBR
|
GAPDH[1]
|
glyceraldehyde-3-phosphate dehy-drogenase
|
- Genotype effect
- Resistant cultivar regent
- Downy Mildew Infection
|
EF192466
|
- F:TCAAGGTCAAGGACTCTAACACC
- R:CCAACAACGAACATAGGAGCA
|
226
|
60
|
SYBR
|
SAND[1]
|
SAND family protein
|
- Susceptible cultivar trincadeira
- Downy Mildew Infection
|
CF405409
|
- F:CAACATCCTTTACCCATTGACAGA
- R:GCATTTGATCCACTTGCAGATAAG
|
76
|
60
|
SYBR
|
Moleculer Type
Evaluation Methods
Contact
- Name: Filipa Monteiro
- Email: fimonteiro@fc.ul.pt
- Institute: Plant Systems Biology Lab, Center of Biodiversity, Functional & Integrative Genomics (BioFIG), Science Faculty of Lisbon University, Lisboa, Portugal
Citation Statistics
Cited by 29 (Based on Google Scholar [2017-06-16])
Involved In Pterostilbene Synthesis
Reference Genes
Gene Symbol
|
Gene Name
|
Application Scope
|
Accession Number
|
Primer
|
Size [bp]
|
Tm [℃]
|
Detection
|
VATP16[2]
|
V-type proton ATPase 16 kDa proteolipid subunit
|
- In grapevine leaves and berries infected by P. viticola and B. cinerea
|
XM_002269086
|
- F:CTTCTCCTGTATGGGAGCTG
- R:CCATAACAACTGGTACAATCGAC
|
112
|
85.23
|
SYBR
|
60SRP[2]
|
60S ribosomal protein L18
|
- In grapevine leaves and berries infected by P. viticola and B. cinerea
|
XM_002270599
|
- F:ATCTACCTCAAGCTCCTAGTC
- R:CAATCTTGTCCTCCTTTCCT
|
165
|
82.39
|
SYBR
|
Moleculer types
Evaluation Methods
Contact
- Name: Marielle Adrian
- Email: marielle.adrian@u-bourgogne.fr
- Institute: Unité Mixte de Recherche INRA 1088/CNRS 5184, Université de Bourgogne Plante-Microbe-Environnement, 17 rue Sully, BP 86510, 21065 Dijon cedex, France
References
- ↑ 1.0 1.1 1.2 1.3
Monteiro F, Sebastiana M, Pais M S, et al. Reference gene selection and validation for the early responses to downy mildew infection in susceptible and resistant Vitis vinifera cultivars[J]. PloS one, 2013, 8(9): e72998.
- ↑ 2.0 2.1
Gamm M, Héloir M C, Kelloniemi J, et al. Identification of reference genes suitable for qRT-PCR in grapevine and application for the study of the expression of genes involved in pterostilbene synthesis[J]. Molecular Genetics and Genomics, 2011, 285(4): 273-285.